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    Microchip large-volume PCR with integrated real-time CE detection
    2.
    发明授权
    Microchip large-volume PCR with integrated real-time CE detection 有权
    Microchip大容量PCR，集成实时CE检测

    公开(公告)号：US08394324B2

    公开(公告)日：2013-03-12

    申请号：US12664018

    申请日：2008-06-11

    申请人： Luc Bousse ,  Jian-ping Zhang

    发明人： Luc Bousse ,  Jian-ping Zhang

    IPC分类号： G01N15/06 ,  G01N33/00 ,  G01N33/48 ,  C12Q1/68 ,  C12P19/34 ,  C12M1/36 ,  C12M1/38 ,  C12M3/00 ,  C12M1/34

    CPC分类号： G01N27/44791 ,  B01L3/502746 ,  B01L3/502753 ,  B01L7/52 ,  B01L2300/0816 ,  B01L2400/0421 ,  G01N27/44721

    摘要： A microfluidic device is provided with appropriate integrated structures to conduct large volume PCR and end-point or real-time capillary electrophoresis detection. The microfluidic device includes a substrate having an amplification chamber of a volume of nucleic acid, wells disposed on the substrate, flow channels connecting the wells and the chamber in the substrate to allow for solution flow through the chamber, and one or more separation channels provided in the substrate and connected to the chamber for separating and detecting a fraction of the amplified nucleic acid. The chamber, the flow channels, and the one or more separation channels are configured such that the hydrodynamic flow resistance of the chambers and the flow channels combined is at least 10Λ3 times smaller than the hydrodynamic flow resistance in the one or more separation channels. The microfluidic device can achieve a very high sensitivity in detection while being highly cost effective.

    摘要翻译： 微流体装置具有适当的整合结构，可进行大容量PCR和终点或实时毛细管电泳检测。 微流体装置包括具有一定体积的核酸的扩增室的衬底，设置在衬底上的阱，连通阱的流动通道和衬底中的室，以允许溶液流过腔室，以及提供一个或多个分离通道 在底物中并连接到室以分离和检测一部分扩增的核酸。 腔室，流动通道和一个或多个分离通道被构造成使得腔室和流动通道组合的流体动力学流动阻力比一个或多个分离通道中的流体动力流动阻力小至少10 ^ 3倍。 微流体装置在检测中可以获得非常高的灵敏度，同时具有高成本效益。