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公开(公告)号：US12037638B2
公开(公告)日：2024-07-16
申请号：US17783231
申请日：2020-12-09
IPC分类号： G06F30/20 , C12Q1/6865 , G16B20/00 , G16B40/00 , C12Q1/70
CPC分类号： C12Q1/6865 , G16B20/00 , G16B40/00 , C12Q1/703 , C12Q1/6865 , C12Q2537/165 , C12Q2545/114 , C12Q2561/113 , C12Q2565/625
摘要： Presented are methods, systems, and software products useful for determining the concentration of an analyte in a fluid specimen used to produce a dried sample, where the dried sample serves as a source of the analyte in a detection and quantification procedure. Particularly illustrated is the use of dried blood spots for quantifying a polynucleotide analyte.
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公开(公告)号：US20190250156A1
公开(公告)日：2019-08-15
申请号：US16307125
申请日：2017-06-07
发明人： Daniel Takashi Kamei , Benjamin Ming Wu , Daniel William Bradbury , Shin Ting Sherine Frieda Cheung
IPC分类号： G01N33/543
CPC分类号： G01N33/54386 , C12Q1/6813 , C12Q1/6844 , G01N33/53 , G01N33/543 , G01N33/54306 , G01N33/558 , C12Q2563/159 , C12Q2565/625 , C12Q2521/513 , C12Q2531/119 , C12Q2531/143
摘要： In various embodiments methods and devices are provided for the detection and/or quantification of an analyte. In certain embodiments a device is provided comprising an aqueous two-phase system (ATPS) comprising a mixed phase solution that separates into a first phase solution and a second phase where, in use, said first phase solution becomes a leading phase and said second phase solution becomes a lagging phase; a lateral-flow assay (LFA); and a probe and/or a development reagent, where in use, said probe associates with said first phase solution in said leading phase of said ATPS and/or said development reagent associates with said second phase solution in said lagging phase of said ATPS. In certain embodiments a “one-pot” system of purifying and amplifying a nucleic acid is provided utilizing, e.g., an ATPS and isothermal amplification reagents.
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公开(公告)号：US20180148774A1
公开(公告)日：2018-05-31
申请号：US15574750
申请日：2016-05-16
申请人： GODX, INC , THE UNITED STATES OF AMERICA AS REPRESENTED BY THE SECRETARY, DEPARTMENT OF HUMAN SERVICES
IPC分类号： C12Q1/6834 , B01L3/00 , C12Q1/70 , C12Q1/6888 , C12Q1/6825
CPC分类号： C12Q1/6834 , B01L3/5023 , B01L2200/141 , B01L2300/069 , B01L2400/0406 , C12Q1/00 , C12Q1/6816 , C12Q1/6825 , C12Q1/6888 , C12Q1/689 , C12Q1/701 , G01N15/06 , G01N15/1056 , G01N15/1463 , G01N15/1484 , G01N33/487 , G01N2015/0693 , G01N2015/1006 , Y02A50/51 , Y02A50/52 , Y02A50/53 , Y02A50/58 , C12Q2565/625
摘要： The present invention relates to the rapid and electricity-free, point-of-care, multiplexed detection and quantification of at least one or more nucleic acid sequences from nucleic acids corresponding to a plurality of pathogens or biomarkers using a micropatterned lateral flow device. Rapid and molecular-level sensitive differential diagnosis of a disease condition may be enabled without the need for a delayed laboratory test so that timely treatment can be administered.
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公开(公告)号：US20180135110A1
公开(公告)日：2018-05-17
申请号：US15832635
申请日：2017-12-05
IPC分类号： C12Q1/6811 , C12Q1/6806 , C12Q1/686
CPC分类号： C12Q1/6811 , B01L3/5027 , C12N2310/16 , C12N2310/3519 , C12Q1/6806 , C12Q1/6834 , C12Q1/686 , C12Q2600/158 , G01N33/54366 , C12Q2523/32 , C12Q2525/205 , C12Q2565/625 , C12Q2565/629
摘要： Devices for detecting targets in a sample and methods for detecting such targets are discussed. The targets may be biomarkers associated with a disease or other health condition. The devices may contain a disc including a plurality of microfluidic channels each extending in a radial direction of the disc, the microfluidic channels containing a plurality of capture molecules specific to at least one target. The capture molecules may include an aptamer and/or an oligonucleotide capable of hybridizing to the target. The methods may include introducing a fluid sample into one or more microfluidic channels of a disc, rotating the disc, such that the fluid sample flows radially outward through the microfluidic channel(s) to combine with capture molecules in the disc, and detecting a signal from the disc indicative of a presence of the target.
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公开(公告)号：US09920356B2
公开(公告)日：2018-03-20
申请号：US13989207
申请日：2011-11-24
CPC分类号： C12Q1/6834 , C12Q1/6832 , C12Q1/686 , G01N33/54313 , C12Q2525/155 , C12Q2525/161 , C12Q2565/519 , C12Q2525/186 , C12Q2531/113 , C12Q2565/625
摘要： An object of the invention is to provide a nucleic acid detection method which takes advantage of the high specificity of hybridization techniques, reduces the time length and the number of steps required for detection of PCR products, and allows for easy and highly accurate detection by visual observation without the need of special equipment; and a nucleic acid detection device or kit. The invention provides a method for detecting a target nucleic acid in a sample, which includes performing amplification of the target nucleic acid sequence to synthesize an amplification product having a partially double-stranded structure where a single-stranded region is added to each end of the target sequence, and hybridizing a nucleic acid sequence bound to a development medium and a nucleic acid sequence labeled with a labeling compound with the single-stranded regions of the amplification product to form a sandwich hybridization complex; and a detection device thereof.
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公开(公告)号：US09816136B1
公开(公告)日：2017-11-14
申请号：US15614626
申请日：2017-06-06
CPC分类号： C12Q1/686 , B01L3/502 , B01L7/52 , C12P19/34 , C12Q1/6844 , C12Q1/6848 , C12Q1/6851 , C12Q2537/149 , C12Q2547/101 , C12Q2565/625
摘要： The present invention discloses a two-stage reaction and detection tube comprises a first tube, a second tube and a connector. The first tube comprises a detection space for placing a dipstick and a detection space for the test result. The second tube comprises a storing space for the PCR or RT-PCR reagents and the target gene segments. The connector comprises a first portion and a second portion which connect to the first tube and the second tube respectively. The connector further comprises a diversion unit, a liquid collection space, and a dipstick fixing space, where the liquid collection space is connected to the dipstick fixing space. The target gene amplification and detection could be directly processed in the same tube without any liquid transfer.
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公开(公告)号：US09783844B2
公开(公告)日：2017-10-10
申请号：US14396466
申请日：2013-04-26
申请人： Kaneka Corporation
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6853 , C12Q1/6834 , C12Q2525/186 , C12Q2525/197 , C12Q2563/131 , C12Q2565/625
摘要： An object of the present invention is to provide methods for amplifying and detecting a nucleic acid that allow efficient hybridization, and devices and kits for use in the methods. The present invention includes amplifying a target nucleic acid into a double-stranded nucleic acid having a single-stranded region at each end, and detecting this nucleic acid. The present invention also provides detection devices and kits that make use of these methods.
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8.发明申请公开(公告)号：US20170218434A1
公开(公告)日：2017-08-03
申请号：US15488204
申请日：2017-04-14
申请人： HELIXBIND
发明人： Alon Singer
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689 , C12Q1/6816 , C12Q1/6883 , C12Q1/6895 , C12Q2600/158 , C12Q2600/16 , C12Q2525/107 , C12Q2537/143 , C12Q2565/519 , C12Q2565/625
摘要： The present disclosure provides for compositions of γPNA probes. Additionally, the present disclosure provide for methods and kits using γPNA probes for the diagnosis of sepsis.
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公开(公告)号：US20160002621A1
公开(公告)日：2016-01-07
申请号：US14321160
申请日：2014-07-01
发明人： John Richard Nelson , David Roger Moore , Robert Scott Duthie , Matthew Jeremiah Misner , Gregory Andrew Grossmann , Elizabeth Marie Dees , Patrick McCoy Spooner , Erik Leeming Kvam , Andrew Arthur Paul Burns , Vicki Herzl Watkins
IPC分类号： C12N15/10
CPC分类号： C12N15/1006 , C12Q1/6834 , C12Q2565/625
摘要： A method is provided herein, the method includes: applying a sample comprising target nucleic acids to a sample application zone of a substrate; and flowing a nucleic acid amplification reaction mixture across a length of the substrate through the sample application zone to amplify the target nucleic acid forming a nucleic acid amplification product; wherein the target nucleic acid having a first molecular weight is substantially immobilized at the sample application zone and wherein the amplification product having a second molecular weight migrates away from the sample application zone. An associated device is also provided.
摘要翻译： 本文提供了一种方法，所述方法包括：将包含靶核酸的样品施加到底物的样品施用区; 并使核酸扩增反应混合物通过样品施用区跨越一定长度的底物扩增形成核酸扩增产物的靶核酸; 其中具有第一分子量的靶核酸基本上固定在样品施用区，并且其中具有第二分子量的扩增产物从样品施用区迁移。 还提供了相关联的设备。
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10.发明申请公开(公告)号：US20150355132A1
公开(公告)日：2015-12-10
申请号：US13865352
申请日：2013-04-18
IPC分类号： G01N27/327 , G01N33/543 , B01L3/00
CPC分类号： G01N27/3276 , B01L3/5023 , B01L3/502707 , B01L3/502715 , B01L3/50273 , B01L3/502738 , B01L3/5055 , B01L2200/12 , B01L2300/045 , B01L2300/0627 , B01L2300/0645 , B01L2300/0816 , B01L2300/0877 , B01L2300/126 , B01L2400/0406 , B01L2400/065 , C12Q1/001 , C12Q1/6813 , G01N33/48707 , G01N33/54373 , G01N33/5438 , G01N2035/00158 , Y10T29/49984 , C12Q2565/625
摘要： Described herein are three-dimensional (3-D) paper fluidic devices. The entire 3-D device is fabricated on a support layer formed from a single sheet of material and assembled by folding the support layer. The folded structure may be enclosed in an impermeable cover or package. Chemically sensitive particles may be disposed in the support layer for use in detecting analytes.
摘要翻译： 本文描述了三维（3-D）纸流体装置。 整个3-D器件制造在由单片材料形成并由折叠支撑层组装的支撑层上。 折叠的结构可以封闭在不可渗透的盖或包装中。 化学敏感性颗粒可以设置在支撑层中，用于检测分析物。
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