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    Probe composition and method
    11.
    发明申请
    Probe composition and method 失效
    探头组成和方法

    公开(公告)号：US20050112609A1

    公开(公告)日：2005-05-26

    申请号：US10825624

    申请日：2004-04-14

    Applicant: Paul Grossman ,  Steven Fung ,  Steven Menchen ,  Sam Woo ,  Emily Winn-Deen

    Inventor： Paul Grossman ,  Steven Fung ,  Steven Menchen ,  Sam Woo ,  Emily Winn-Deen

    IPC: C12Q1/68

    CPC classification number: C12Q1/6816 ,  C12Q1/6825 ,  C12Q1/6827 ,  C12Q1/686 ,  C12Q1/6862 ,  C12Q2600/156 ,  G01N27/447 ,  Y10T436/143333 ,  C12Q2525/197 ,  C12Q2525/203 ,  C12Q2537/143 ,  C12Q2565/131 ,  C12Q2565/125 ,  C12Q2565/107 ,  C12Q2521/501 ,  C12Q2563/155 ,  C12Q2563/119 ,  C12Q2549/119

    Abstract: Method and composition for detecting one or more selected polynucleotide regions in a target polynucleotide. In the method, a mixture of sequence-specific probes are reacted with the target polynucleotide under hybridization conditions, and the hybridized probes are treated to selectively modify those probes which are bound to the target polynucleotide in a base-specific manner. The resulting labeled probes include a polymer chain which imparts to each different-sequence probe, a distinctive ratio of charge/translational frictional drag, and a detectable label. The labeled probes are fractionated by electrophoresis in a non-sieving matrix, and the presence of one or more selected sequences in the target polynucleotide are detected according to the observed electrophoretic migration rates of the labeled probes in a non-sieving medium.

    Abstract translation: 用于检测靶多核苷酸中的一个或多个选择的多核苷酸区域的方法和组合物。 在该方法中，将序列特异性探针的混合物与靶多核苷酸在杂交条件下反应，并对杂交的探针进行处理以选择性地修饰以碱基特异性方式结合靶多核苷酸的那些探针。 所得到的标记探针包括赋予每个不同序列探针的聚合物链，电荷/平移摩擦阻力的独特比例和可检测标记。 标记的探针通过在非筛选基质中的电泳分级分离，并且根据在非筛选培养基中标记的探针的观察到的电泳迁移速率来检测靶多核苷酸中一个或多个选定序列的存在。

    Automatic tape searching method
    12.
    发明授权
    Automatic tape searching method 失效
    自动磁带搜索方法

    公开(公告)号：US5287232A

    公开(公告)日：1994-02-15

    申请号：US412005

    申请日：1989-09-25

    Applicant: Jong-Sam Woo

    Inventor： Jong-Sam Woo

    IPC: G11B15/02 ,  G11B15/18 ,  G11B27/10

    CPC classification number: G11B15/1875 ,  G11B15/026 ,  G11B27/107 ,  G11B2220/90

    Abstract: An automatic (auto) tape searching method which searches the required tape portion fast and automatically by repeating a sequence of operational procedures. The auto tape searching method comprises: a first stage which initializes the port of a microcomputer and the content of a RAM (random access memory), sets the initial values, and controls interrupts at a power-on reset of a video tape recording system; a second stage which checks key input and, for auto random search (ARS) key, performs the auto random search function; a third stage which performs mode checking and, if a present mode needs to accompany the mechanism operation, controls the present mode; and a fourth stage which switches the mode and checks the sensor and an emergency state to make the system in stable condition.

    Abstract translation: 一种自动（自动）磁带搜索方法，通过重复一系列操作程序快速和自动地搜索所需的磁带部分。 自动磁带搜索方法包括：初始化微型计算机端口的第一级和RAM（随机存取存储器）的内容，设置初始值，并控制录像机系统的上电复位时的中断; 检查密钥输入的第二阶段，并且对于自动随机搜索（ARS）密钥执行自动随机搜索功能; 执行模式检查的第三级，并且如果当前模式需要伴随机构操作，则控制当前模式; 以及切换模式并检查传感器和紧急状态以使系统处于稳定状态的第四阶段。

    Nanowire-based system for analysis of nucleic acids
    13.
    发明授权
    Nanowire-based system for analysis of nucleic acids 有权
    基于纳米线的核酸分析系统

    公开(公告)号：US08703497B2

    公开(公告)日：2014-04-22

    申请号：US13487638

    申请日：2012-06-04

    Applicant: Hongye Sun ,  Steven Fung ,  Sam Woo

    Inventor： Hongye Sun ,  Steven Fung ,  Sam Woo

    IPC: G01N27/00 ,  G01N27/403 ,  C12M1/34 ,  B82Y15/00 ,  G01N27/04 ,  G01N27/22

    CPC classification number: C12Q1/6874 ,  C12Q1/6811 ,  C12Q1/6869 ,  G01N27/3276 ,  G01N27/3278 ,  Y10T436/14 ,  Y10T436/142222 ,  Y10T436/143333 ,  C12Q2565/607 ,  C12Q2533/101 ,  C12Q2565/133 ,  C12Q2563/155

    Abstract: A system and method for analyzing nucleic acids is presented herein. The system can include a nanowire assembly including a plurality of nanowires. In some embodiments, at least two nanowires can be coupled to a different probe corresponding to a different sequence version of a polymorphism. The system can include a reagent delivery system capable of delivering a template polymorphic nucleotide, an extension nucleic acid, and an enzyme to the nanowire assembly. The system can include an electrical detector configured to measure an electrical characteristic of the nanowire assembly and/or a controller in communication with the reagent delivery system. In some embodiments, the electrical detector can be configured to collect data corresponding to changes in the electrical characteristic in response to contact with the template polymorphic nucleotide, the extension nucleic acid, and the enzyme.

    Abstract translation: 本文提供了用于分析核酸的系统和方法。 该系统可以包括包括多个纳米线的纳米线组件。 在一些实施例中，至少两个纳米线可以耦合到对应于多态性的不同序列形式的不同探针。 该系统可以包括能够将模板多核苷酸，延伸核酸和酶递送至纳米线组件的试剂递送系统。 该系统可以包括被配置为测量纳米线组件和/或与试剂递送系统连通的控制器的电特性的电检测器。 在一些实施方案中，电检测器可被配置为响应于与模板多核苷酸，延伸核酸和酶的接触而收集对应于电特性变化的数据。

    CHEMICALLY-ENHANCED PRIMER COMPOSITIONS, METHODS AND KITS
    14.
    发明申请
    CHEMICALLY-ENHANCED PRIMER COMPOSITIONS, METHODS AND KITS 有权
    化学增强的组合物，方法和工具

    公开(公告)号：US20120270211A1

    公开(公告)日：2012-10-25

    申请号：US13397626

    申请日：2012-02-15

    Applicant: Linda LEE ,  Sam WOO ,  Peter MA

    Inventor： Linda LEE ,  Sam WOO ,  Peter MA

    IPC: C12Q1/68 ,  C07H21/04

    CPC classification number: C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2525/113 ,  C12Q2525/125

    Abstract: A chemically-enhanced primer is provided comprising a negatively charged moiety (NCM), an oligonucleotide sequence having a) non-nuclease resistant inter-nucleotide linkages or b) at least one nuclease resistance inter-nucleotide linkage. The chemically-enhanced primer can be used for sequencing and fragment analysis. Methods for synthesizing the chemically-enhanced primer as well as a method of preparing DNA for sequencing, a method of sequencing DNA, and kits containing the chemically-enhanced primer are also provided. The method of sequencing DNA can comprise contacting amplification reaction products with the composition wherein excess amplification primer is degraded by the nuclease and the chemically-enhanced primer is essentially non-degraded.

    Abstract translation: 提供化学增强的引物，其包含带负电荷的部分（NCM），具有a）非核酸酶抗性核苷酸间连接的寡核苷酸序列或b）至少一个核酸酶抗性核苷酸间连接。 化学增强引物可用于测序和片段分析。 还提供了用于合成化学增强引物的方法以及制备用于测序的DNA的方法，测序DNA的方法和含有化学增强的引物的试剂盒。 测序DNA的方法可以包括使扩增反应产物与其中过量扩增引物被核酸酶降解并且化学增强引物基本上未降解的组合物接触。

    Mobility-modified nucleobase polymers and methods of using same
    17.
    发明申请
    Mobility-modified nucleobase polymers and methods of using same 有权
    流动性修饰的核碱基聚合物及其使用方法

    公开(公告)号：US20050042644A1

    公开(公告)日：2005-02-24

    申请号：US10856752

    申请日：2004-05-28

    Applicant: Sam Woo ,  Ron Graham ,  Jing Tian

    Inventor： Sam Woo ,  Ron Graham ,  Jing Tian

    IPC: G01N33/53 ,  C07H21/00 ,  C08G65/335 ,  C12N15/09 ,  C12Q1/68 ,  G01N27/447 ,  G01N37/00 ,  C08G63/91

    CPC classification number: C12Q1/6813 ,  C07H21/04 ,  C12Q2565/137 ,  C12Q2525/197

    Abstract: The present invention relates generally to nucleobase polymer functionalizing reagents, to mobility-modified sequence-specific nucleobase polymers, to compositions comprising a plurality of mobility-modified sequence-specific nucleobase polymers, and to the use of such polymers and compositions in a variety of assays, such as, for example, for the detection of a plurality of selected nucleotide sequences within one or more target nucleic acids. The mobility-modifying polymers of the present invention include phosphoramidite reagents which can be joined to other mobility-modifying monomers and to sequence-specific oligonucleobase polymers via uncharged phosphate triester linkages. Addition of the mobility-modifying phosphoramidite reagents of the present invention to oligonucleobase polymers results in unexpectedly large effects the mobility of those modified oligonucleobase polymers, especially upon capillary electrophoresis in non-sieving media.

    Abstract translation: 本发明一般涉及核碱基聚合物官能化试剂，迁移率修饰的序列特异性核碱基聚合物，包含多种迁移率修饰的序列特异性核碱基聚合物的组合物，以及这些聚合物和组合物在多种测定中的用途 ，例如用于检测一个或多个靶核酸内的多个选择的核苷酸序列。 本发明的迁移率改性聚合物包括亚磷酰胺试剂，其可以通过不带电荷的磷酸三酯键连接到其它迁移率改性单体和序列特异性寡核苷酸聚合物。 将本发明的迁移率修饰亚磷酰胺试剂加入到寡核苷酸聚合物中导致这些修饰的寡核苷酸聚合物的迁移率出人意料地大的影响，特别是在非筛选培养基中的毛细管电泳。

    Screen encore method by reel-sensing in a video tape recorder system
    18.
    发明授权
    Screen encore method by reel-sensing in a video tape recorder system 失效
    在磁带录像机系统中通过卷轴感测的屏幕加密方法

    公开(公告)号：US5400150A

    公开(公告)日：1995-03-21

    申请号：US418219

    申请日：1989-10-06

    Applicant: Jong-Sam Woo

    Inventor： Jong-Sam Woo

    IPC: G11B15/02 ,  G11B15/03 ,  G11B15/093 ,  G11B15/18 ,  G11B15/20 ,  G11B27/00 ,  G11B27/028 ,  G11B27/34 ,  G11B27/36

    CPC classification number: G11B15/1875 ,  G11B15/023 ,  G11B15/026 ,  G11B15/03 ,  G11B15/20 ,  G11B27/007 ,  G11B27/028 ,  G11B27/34 ,  G11B27/36 ,  G11B2220/90

    Abstract: A screen encore method capable of displaying again (encore) a block of pictures designated by a user or a last picture with a single key by software control, without adding hardware, so that the inventive system is highly improved both in its function and quality. The inventive system for embodying the method includes: a micom for controlling basic functions of the Video Tape Recorder (VTR) system and functions of the inventive subject; a delay device for indicating to the user the status of the VTR system under the control of the micom; a mechanism for implementing the insertion/ejection of video tapes and tape loading/unloading by the control signals coming from the micom; a video and audio signal controller for controlling video and audio signals under the control of the micom; a remote control receiver for receiving remote control signals from a remote control set to filter and amplify the signals, thereby to transmit them the micom; a sensor for sensing the present operational location of the mechanism and the status of the beginning/end of tapes to transfer the sensed data to the micom; and a keyboard, as a key input running the VTR's functions, for inputting function key and command key to the micom including an encore key.

    Abstract translation: 一种屏幕加密方法，其能够通过软件控制再次（加密）由用户指定的图片块或最后图片，而不添加硬件，使得本发明的系统在其功能和质量方面都得到高度改进。 用于实现该方法的本发明的系统包括：用于控制录像机（VTR）系统的基本功能的微型计算机和本发明的主题的功能; 用于向用户指示在微机控制下的VTR系统的状态的延迟装置; 用于通过来自微型计算机的控制信号实现录像带的插入/弹出和磁带加载/卸载的机构; 用于在微机控制下控制视频和音频信号的视频和音频信号控制器; 遥控接收器，用于接收来自遥控器的遥控信号以对信号进行滤波和放大，从而将其传送到微型计算机; 用于感测机构的当前操作位置的传感器和磁带的开始/结束的状态以将感测到的数据传送到微型计算机; 以及键盘，作为运行VTR功能的键输入，用于将功能键和命令键输入到包括密钥的微机。

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