公开(公告)号：US20110076770A1

公开(公告)日：2011-03-31

申请号：US12892223

申请日：2010-09-28

申请人： Yuhiro SAKAI ,  Katsuyuki Yamanaka ,  Mika Takeda ,  Tomohisa Okura ,  Koichiro Tsuji

发明人： Yuhiro SAKAI ,  Katsuyuki Yamanaka ,  Mika Takeda ,  Tomohisa Okura ,  Koichiro Tsuji

IPC分类号： C12N5/077

CPC分类号： C12N5/0663 ,  C12N2500/50

摘要： To efficiently select and proliferate the mesenchymal stem cells without necessity of an exclusive separating device and a complicated separating operation, mesenchymal stem cells are cultured by seeding at least one of a bone marrow solution, an umbilical cord blood, a peripheral blood, a synovial membrane and an amniotic membrane in a liquid culture medium which is filled in a vessel, includes water as its main components and having a specific gravity between 1.06 and 1.10 g/ml at 37° C., and making a culture at a temperature 37±2° C. on a ceiling side surface of the vessel, preferably the specific gravity being regulated by use of at least one selected from silica fine powder coated by polyvinyl pyrrolidone, a water soluble copolymer of sucrose and epichlorohydrin, and a water soluble compound including a triiodo aromatic ring.

摘要翻译： 为了有效地选择和增殖间充质干细胞而不需要专门的分离装置和复杂的分离操作，通过种植骨髓溶液，脐带血，外周血，滑膜中的至少一种来培养间充质干细胞 在装在容器中的液体培养基中的羊膜包含水作为主要成分，在37℃下比重为1.06〜1.10g / ml，培养温度为37±2℃ ℃，优选通过使用选自由聚乙烯吡咯烷酮涂覆的二氧化硅细粉末，蔗糖和表氯醇的水溶性共聚物和水溶性化合物中选择的至少一种来调节比重，所述水溶性化合物包括 三碘芳环。

公开(公告)号：US20100279412A1

公开(公告)日：2010-11-04

申请号：US12160481

申请日：2007-01-11

申请人： Yukio Kato ,  Jin Chang Shao ,  Yuki Katsura ,  Koichiro Tsuji

发明人： Yukio Kato ,  Jin Chang Shao ,  Yuki Katsura ,  Koichiro Tsuji

IPC分类号： C12N5/02

CPC分类号： C12N5/0663 ,  C12N5/0031 ,  C12N5/0037 ,  C12N5/0686 ,  C12N2500/14 ,  C12N2500/16 ,  C12N2500/30 ,  C12N2500/32 ,  C12N2500/36 ,  C12N2500/38 ,  C12N2500/90 ,  C12N2501/10 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/119 ,  C12N2501/12 ,  C12N2501/135 ,  C12N2501/15 ,  C12N2501/165 ,  C12N2501/33 ,  C12N2501/39 ,  C12N2501/40

摘要： Disclosed are: a culture medium containing a specific growth factor and at least one phospholipid; a composition for preparation of the culture medium; a kit; and a method. A technique can be provided which uses a serum-free or low-serum culture medium and has a promoting effect on the proliferation of an animal cell comparable to the promoting effect obtained by the culture in a serum-containing culture medium.

摘要翻译： 公开了：含有特定生长因子和至少一种磷脂的培养基; 用于制备培养基的组合物; 一套工具 和一种方法。 可以提供一种技术，其使用无血清或低血清培养基，并且与在含血清培养基中培养获得的促进作用相当，对动物细胞的增殖具有促进作用。

公开(公告)号：US20070011753A1

公开(公告)日：2007-01-11

申请号：US11517698

申请日：2006-09-08

申请人： Mamoru Ito ,  Kimio Kobayashi ,  Tatsutoshi Nakahata ,  Koichiro Tsuji ,  Sonoko Habu ,  Yoshio Koyanagi ,  Naoki Yamamoto ,  Kazuo Sugamura ,  Kiyoshi Ando ,  Tatsuji Nomura

发明人： Mamoru Ito ,  Kimio Kobayashi ,  Tatsutoshi Nakahata ,  Koichiro Tsuji ,  Sonoko Habu ,  Yoshio Koyanagi ,  Naoki Yamamoto ,  Kazuo Sugamura ,  Kiyoshi Ando ,  Tatsuji Nomura

IPC分类号： A01K67/027

CPC分类号： C07K16/18 ,  A01K67/027 ,  A01K67/0271 ,  A01K2267/03 ,  A01K2267/0331 ,  A01K2267/0337 ,  A61K39/00

摘要： The present invention provides an immunodeficient mouse (NOG mouse) suitable for engraftment, differentiation and proliferation of heterologous cells, and a method of producing such a mouse. This mouse is obtained by backcrossing a C.B-17-scid mouse with an NOD/Shi mouse, and further backcrossing an interleukin 2-receptor γ-chain gene-knockout mouse with the thus backcrossed mouse. It is usable for producing a human antibody and establishing a stem cell assay system, a tumor model and a virus-infection model.

摘要翻译： 本发明提供了适合于异源细胞的移植，分化和增殖的免疫缺陷小鼠（NOG小鼠），以及这种小鼠的制备方法。 该小鼠通过用NOD / Shi小鼠回交C.B-17- scid小鼠并通过回交的小鼠进一步回交白细胞介素2受体γ链基因敲除小鼠而获得。 它可用于产生人抗体并建立干细胞测定系统，肿瘤模型和病毒感染模型。

公开(公告)号：US09394521B2

公开(公告)日：2016-07-19

申请号：US13583150

申请日：2011-03-10

申请人： Toshie Tsuchiya ,  Koichiro Tsuji ,  Yukio Kato ,  Jin Chang Shao ,  Maiko Hara

发明人： Toshie Tsuchiya ,  Koichiro Tsuji ,  Yukio Kato ,  Jin Chang Shao ,  Maiko Hara

IPC分类号： C12N5/0775 ,  C12Q1/06 ,  A61K35/12

CPC分类号： C12N5/0663 ,  A61K35/12 ,  C12N5/0668 ,  C12N2500/90 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/12 ,  C12N2501/135 ,  C12N2501/15

摘要： A cell preparation containing mesenchymal stem cells whose immunosuppression ability is maintained is produced by means of a serum-free or low-serum culture. A method for producing a cell preparation containing mesenchymal stem cells, comprising the steps of: (A) proliferating mesenchymal stem cells in a serum-free medium “A” containing an FGF, a PDGF, a TGF-β, an HGF, an EGF, at least one phospholipid, and at least one fatty acid; and (B) screening mesenchymal stem cells whose immunosuppression ability is maintained or improved, from the mesenchymal stem cells thus proliferated in the step (A).

摘要翻译： 通过无血清或低血清培养物产生含有维持免疫抑制能力的间充质干细胞的细胞制剂。 一种含有间充质干细胞的细胞制剂的制备方法，包括以下步骤：（A）在含有FGF，PDGF，TGF-βb，HGF，HGF的无血清培养基“A”中增殖间充质干细胞 EGF，至少一种磷脂和至少一种脂肪酸; 和（B）从步骤（A）中因此增殖的间充质干细胞筛选其免疫抑制能力维持或改善的间充质干细胞。

公开(公告)号：US09074176B2

公开(公告)日：2015-07-07

申请号：US12160481

申请日：2007-01-11

申请人： Yukio Kato ,  Jin Chang Shao ,  Yuki Katsura ,  Koichiro Tsuji

发明人： Yukio Kato ,  Jin Chang Shao ,  Yuki Katsura ,  Koichiro Tsuji

IPC分类号： C12N5/02 ,  C12N5/00

CPC分类号： C12N5/0663 ,  C12N5/0031 ,  C12N5/0037 ,  C12N5/0686 ,  C12N2500/14 ,  C12N2500/16 ,  C12N2500/30 ,  C12N2500/32 ,  C12N2500/36 ,  C12N2500/38 ,  C12N2500/90 ,  C12N2501/10 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/119 ,  C12N2501/12 ,  C12N2501/135 ,  C12N2501/15 ,  C12N2501/165 ,  C12N2501/33 ,  C12N2501/39 ,  C12N2501/40

摘要： Disclosed are: a culture medium containing a specific growth factor and at least one phospholipid; a composition for preparation of the culture medium; a kit; and a method. A technique can be provided which uses a serum-free or low-serum culture medium and has a promoting effect on the proliferation of an animal cell comparable to the promoting effect obtained by the culture in a serum-containing culture medium.

摘要翻译： 公开了：含有特定生长因子和至少一种磷脂的培养基; 用于制备培养基的组合物; 一套工具 和一种方法。 可以提供一种技术，其使用无血清或低血清培养基，并且与在含血清培养基中培养获得的促进作用相当，对动物细胞的增殖具有促进作用。

公开(公告)号：US08119397B2

公开(公告)日：2012-02-21

申请号：US10594595

申请日：2005-03-31

申请人： Yukio Kato ,  Masahiro Nishimura ,  Yoshie Ozaki ,  Koichiro Tsuji

发明人： Yukio Kato ,  Masahiro Nishimura ,  Yoshie Ozaki ,  Koichiro Tsuji

IPC分类号： C12N5/00 ,  A61K38/16 ,  C07K1/00

CPC分类号： A61K31/728 ,  A61K35/28 ,  C12N5/0663 ,  C12N2501/105 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/12 ,  C12N2501/135 ,  C12N2501/15 ,  C12N2501/905 ,  C12N2501/998 ,  A61K2300/00

摘要： This invention provides therapeutic agents, transplants and therapeutic methods that can enhance the regeneration of injured tissue. This invention relates to agents, transplants and therapeutic methods for enhancing the migration and accumulation of mesenchymal stem cells in injured tissues and/or suppressing the diffusion of mesenchymal stem cells from injured tissues.

摘要翻译： 本发明提供可以增强损伤组织再生的治疗剂，移植物和治疗方法。 本发明涉及用于增强受损组织中间充质干细胞的迁移和积累和/或抑制间充质干细胞从损伤组织扩散的药剂，移植物和治疗方法。

公开(公告)号：US20100158982A1

公开(公告)日：2010-06-24

申请号：US12709748

申请日：2010-02-22

申请人： Toshitsugu KAWATA ,  Kaoru Tenjo ,  Koichiro Tsuji ,  Katsuyuki Yamanaka

发明人： Toshitsugu KAWATA ,  Kaoru Tenjo ,  Koichiro Tsuji ,  Katsuyuki Yamanaka

IPC分类号： A61K35/28 ,  A61K9/70 ,  A61P19/00 ,  A61P21/00

CPC分类号： A61L27/3804 ,  A61F2/2846 ,  A61F2/3094 ,  A61F2002/30062 ,  A61F2210/0004 ,  A61K35/35 ,  A61K2035/124 ,  A61L27/3633 ,  A61L27/3641 ,  A61L27/3839 ,  A61L31/005 ,  C12N5/0663 ,  C12N2533/40

摘要： To provide a sheet for guiding regeneration of mesenchymal tissue having improved formability, strength, absorbability and efficiency, the sheet is produced by steps of containing a culture medium in a surface of a porous sheet produced by freezing a bioabsorbable polymer material dissolved with an organic solvent and drying it, seeding a mesenchymal cell grown after taking from biotissue, and differentiating the mesenchymal cell to a mesenchymal tissue precursor cell, where the mesenchymal tissue precursor cell and an extracellular substrate are adhered on the surface of the porous sheet containing a culture medium, and the extracellular substrate is secreted in a process of the mesenchymal cell being differentiated to the mesenchymal tissue precursor cell.

摘要翻译： 为了提供一种用于引导具有改善的成形性，强度，吸收性和效率的间充质组织再生的片材，该片材通过以下步骤制备：在通过冷冻溶解有有机溶剂的生物可吸收聚合物材料制成的多孔片材的表面中含有培养基 并将其干燥，接种从生物组织取出后生长的间充质细胞，并将间充质细胞分化为间充质组织前体细胞，其中间充质组织前体细胞和细胞外基质粘附在含有培养基的多孔片材的表面上， 并且细胞外基质在分化成间充质组织前体细胞的间充质细胞的过程中分泌。

公开(公告)号：US20080220521A1

公开(公告)日：2008-09-11

申请号：US12123572

申请日：2008-05-20

申请人： Toshitsugu Kawata ,  Kaoru Tenjo ,  Koichiro Tsuji ,  Katsuyuki Yamanaka

发明人： Toshitsugu Kawata ,  Kaoru Tenjo ,  Koichiro Tsuji ,  Katsuyuki Yamanaka

IPC分类号： C12N5/02

CPC分类号： A61L27/3804 ,  A61F2/2846 ,  A61F2/3094 ,  A61F2002/30062 ,  A61F2210/0004 ,  A61K35/35 ,  A61K2035/124 ,  A61L27/3633 ,  A61L27/3641 ,  A61L27/3839 ,  A61L31/005 ,  C12N5/0663 ,  C12N2533/40

摘要： To provide a sheet for guiding regeneration of mesenchymal tissue having improved formability, strength, absorbability and efficiency, the sheet is produced by steps of containing a culture medium in a surface of a porous sheet produced by freezing a bioabsorbable polymer material dissolved with an organic solvent and drying it, seeding a mesenchymal cell grown after taking from biotissue, and differentiating the mesenchymal cell to a mesenchymal tissue precursor cell, where the mesenchymal tissue precursor cell and an extracellular substrate are adhered on the surface of the porous sheet containing a culture medium, and the extracellular substrate is secreted in a process of the mesenchymal cell being differentiated to the mesenchymal tissue precursor cell.

摘要翻译： 为了提供一种用于引导具有改善的成形性，强度，吸收性和效率的间充质组织再生的片材，该片材通过以下步骤制备：在通过冷冻溶解有有机溶剂的生物可吸收聚合物材料制成的多孔片材的表面中含有培养基 并将其干燥，接种从生物组织取出后生长的间充质细胞，并将间充质细胞分化为间充质组织前体细胞，其中间充质组织前体细胞和细胞外基质粘附在含有培养基的多孔片材的表面上， 并且细胞外基质在分化成间充质组织前体细胞的间充质细胞的过程中分泌。

公开(公告)号：US20060166214A1

公开(公告)日：2006-07-27

申请号：US10548681

申请日：2004-02-27

申请人： Yukio Kato ,  Koichiro Tsuji ,  Chika Koike

发明人： Yukio Kato ,  Koichiro Tsuji ,  Chika Koike

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C12N15/1138 ,  C07K14/47 ,  C12Q1/6881 ,  C12Q2600/158

摘要： The present invention provides a marker for detecting, separating/distinguishing a mesenchymal stem cell and a method for detecting, separating/distinguishing a mesenchymal stem cell by using the marker. A gene shown in the sequence listing is expressed specifically in a mesenchymal stem cell. The present invention comprises use of the gene as a marker for detecting mesenchymal stem cells. In addition, the present invention comprises a probe for detecting the mesenchymal stem cell marker gene and a PCR primer for amplifying the gene in a test cell in detecting the mesenchymal stem cell gene marker, and further comprises a polypeptide marker for detecting mesenchymal stem cells comprising a polypeptide wherein the mesenchymal stem cell marker gene of the present invention is expressed, an antibody for detecting the polypeptide marker which specifically binds to the polypeptide marker, and still further, a method for distinguishing and separating a mesenchymal stem cell by using a probe for detecting the mesenchymal stem cell marker gene, and an antibody which specifically binds to a polypeptide marker.

摘要翻译： 本发明提供了用于检测，分离/区分间充质干细胞的标记物和通过使用标记物检测，分离/鉴别间充质干细胞的方法。 序列表中所示的基因在间充质干细胞中特异性表达。 本发明包括使用该基因作为检测间充质干细胞的标志物。 此外，本发明包括用于检测间充质干细胞标记基因的探针和用于在检测间充质干细胞基因标记物中扩增测试细胞中的基因的PCR引物，并且还包含用于检测间充质干细胞的多肽标记，包括 其中表达本发明的间充质干细胞标记基因的多肽，用于检测与多肽标记特异性结合的多肽标记物的抗体，还有，通过使用探针来鉴别和分离间充质干细胞的方法 检测间充质干细胞标记基因，以及特异性结合多肽标记的抗体。

公开(公告)号：US06733954B2

公开(公告)日：2004-05-11

申请号：US10396419

申请日：2003-03-26

申请人： Kunitoshi Yamamoto ,  Koichiro Tsuji

发明人： Kunitoshi Yamamoto ,  Koichiro Tsuji

IPC分类号： G03C556

CPC分类号： H01L23/13 ,  H01L21/561 ,  H01L23/3121 ,  H01L23/49805 ,  H01L23/49838 ,  H01L23/50 ,  H01L24/48 ,  H01L24/49 ,  H01L24/97 ,  H01L2224/16225 ,  H01L2224/32225 ,  H01L2224/45144 ,  H01L2224/48091 ,  H01L2224/48227 ,  H01L2224/48465 ,  H01L2224/49175 ,  H01L2224/73265 ,  H01L2224/97 ,  H01L2924/00014 ,  H01L2924/01005 ,  H01L2924/01006 ,  H01L2924/01011 ,  H01L2924/01013 ,  H01L2924/01018 ,  H01L2924/01019 ,  H01L2924/01023 ,  H01L2924/01027 ,  H01L2924/01029 ,  H01L2924/01033 ,  H01L2924/01039 ,  H01L2924/01046 ,  H01L2924/01047 ,  H01L2924/01054 ,  H01L2924/01078 ,  H01L2924/01079 ,  H01L2924/01082 ,  H01L2924/07811 ,  H01L2924/12041 ,  H01L2924/12042 ,  H01L2924/1301 ,  H01L2924/15151 ,  H01L2924/181 ,  H01L2924/3025 ,  H05K1/0206 ,  H05K1/141 ,  H05K3/0052 ,  H05K3/0082 ,  H05K3/064 ,  H05K3/108 ,  H05K3/184 ,  H05K3/3442 ,  H05K3/403 ,  H05K2201/049 ,  H05K2201/0959 ,  H05K2201/09645 ,  H05K2203/1407 ,  H01L2224/85 ,  H01L2224/81 ,  H01L2924/00 ,  H01L2924/00012 ,  H01L2224/45099 ,  H01L2224/45015 ,  H01L2924/207

摘要： Through holes are formed at four peripheral edges of a plurality of semiconductor chip placement regions of an insulating substrate, except for coupling portions partially arranged thereat. A substrate sheet for semiconductor module is used in which connecting portions between inner lead portions and outer lead portions arranged on both surfaces of the substrate are formed in pattern on the side wall surface of the through hole. The semiconductor chip is mounted on each region, electrode terminals thereof and the inner lead portions are electrically connected to each other, the chip is sealed, and then the coupling portions are cut.

摘要翻译： 绝缘基板的多个半导体芯片配置区域的四个周缘形成通孔，除了部分地布置在其上的耦合部分之外。 使用半导体模块用基板，其中，设置在基板的两面的内引线部和外引线部之间的连接部形成为贯通孔的侧壁面。 半导体芯片安装在每个区域上，其电极端子和内引线部分彼此电连接，芯片被密封，然后切断连接部分。