公开(公告)号：US09486574B2

公开(公告)日：2016-11-08

申请号：US14063751

申请日：2013-10-25

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Ofer Yodfat ,  Yair Dan ,  Ofer Arnold ,  Avraham Neta ,  Yossi Shalev

IPC: A61M5/315 ,  A61M5/145 ,  A61M5/14 ,  A61M5/142 ,  A61M5/168 ,  A61M5/31

CPC classification number: A61M5/1456 ,  A61M5/1413 ,  A61M5/14248 ,  A61M5/1684 ,  A61M5/16854 ,  A61M5/3129 ,  A61M2005/1402 ,  A61M2005/14252 ,  A61M2005/14268 ,  A61M2005/14573 ,  A61M2005/314 ,  A61M2205/186 ,  A61M2205/3306 ,  A61M2205/3317 ,  A61M2205/3365 ,  A61M2205/3389 ,  A61M2205/3569 ,  A61M2205/3592 ,  A61M2209/045

Abstract: Disclosed is a portable therapeutic fluid dispensing device. The fluid dispensing device includes a reusable part (100) comprising a reusable part housing including at least a controller and a first portion (186) of a driving mechanism, and a disposable part (200) comprising a disposable part housing including at least a reservoir (220) to retain therapeutic fluid, an outlet port to which the therapeutic fluid is dispensed, a fluid conduit providing fluid communication between the reservoir and the outlet port, and a second portion (112) of the driving mechanism. At least a portion of the disposable part housing defines at least a portion of the reservoir. Also, the second portion of the driving mechanism is mechanically couplable to the first portion of the driving mechanism upon connection of the disposable part to the reusable part.

公开(公告)号：US20160319268A1

公开(公告)日：2016-11-03

申请号：US14699299

申请日：2015-04-29

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Werner Hoelke ,  Michaela Fischer ,  Johann-Peter Thalhofer ,  Markus Weber

IPC: C12N9/96 ,  C12N9/54 ,  C12N9/52

CPC classification number: C12N9/96 ,  C12N9/52 ,  C12N9/54 ,  C12N9/6491 ,  C12Y304/24003 ,  C12Y304/24027

Abstract: Described are compositions, in particular lyophilizates, containing proteolytic enzymes, and methods for producing the compositions. Typically these compositions contain one or more proteases with collagenase activity and a neutral protease, for example, thermolysin. The compositions are free of acetate salts. Surprisingly, such compositions can be dissolved in water more rapidly than lyophilized protease mixtures of the state of the art.

Abstract translation: 描述了含有蛋白水解酶的组合物，特别是冻干物，以及用于制备组合物的方法。 通常，这些组合物含有一种或多种具有胶原酶活性的蛋白酶和中性蛋白酶，例如嗜热菌蛋白酶。 组合物不含乙酸盐。 令人惊奇的是，这种组合物可以比现有技术的冻干蛋白酶混合物更快地溶解在水中。

公开(公告)号：US09481902B2

公开(公告)日：2016-11-01

申请号：US14970734

申请日：2015-12-16

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Tibor Czabany ,  Alfred Engel ,  Michael Greif ,  Christine Jung ,  Christiane Luley ,  Sebastian Malik ,  Rainer Mueller ,  Bernd Nidetzky ,  Doris Ribitsch ,  Katharina Schmoelzer ,  Helmut Schwab ,  Harald Sobek ,  Bernhard Suppmann ,  Marco Thomann ,  Sabine Zitzenbacher

IPC: C07K16/00 ,  C12Q1/00 ,  C12P21/00 ,  C12N9/10

CPC classification number: C12P21/005 ,  C07K16/00 ,  C07K2317/14 ,  C07K2317/24 ,  C07K2317/41 ,  C12N9/1081 ,  C12P19/18 ,  C12Y204/99001

Abstract: The present disclosure is directed to the use of certain glycosyltransferase variants having N-terminal truncation deletions. It was found that the combination of two different truncation variants of human β-galactoside-α-2,6-sialyltransferase I (hST6Gal-I) exhibited different specific sialyltransferase enzymatic activities. In one example, under conditions wherein the first variant Δ89 hST6Gal-I catalyzed formation of bi-sialylated target molecules the second variant Δ108 hST6Gal-I catalyzed formation of mono-sialylated target molecules. Thus, disclosed are variants of mammalian glycosyltransferase, nucleic acids encoding the same, methods and means for recombinantly producing the variants of mammalian glycosyltransferase and use thereof, particularly for sialylating in a quantitatively controlled manner terminal acceptor groups of glycan moieties being part of glycoproteins such as immunoglobulins.

Abstract translation: 本公开涉及使用具有N-末端截短缺失的某些糖基转移酶变体。 发现人β-半乳糖苷-α-2,6-唾液酸转移酶I（hST6Gal-1）的两种不同截短变体的组合表现出不同的特异性唾液酸转移酶酶活性。 在一个实例中，在第一变体Δ89hST6Gal-1催化双唾液酸化靶分子的形成的条件下，第二变体Δ108hST6Gal-1催化单唾液酸化靶分子的形成。 因此，公开了哺乳动物糖基转移酶的变体，编码它们的核酸，用于重组产生哺乳动物糖基转移酶变体的方法和手段及其用途，特别是以定量控制的方式唾液酸化作为糖蛋白部分的聚糖部分的末端受体基团，例如 免疫球蛋白。

公开(公告)号：US20160276080A1

公开(公告)日：2016-09-22

申请号：US15170251

申请日：2016-06-01

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Christoph Seidel ,  Jens Christian Bolle ,  Sandra Recklies

IPC: H01F1/44 ,  C08F257/00 ,  C08K9/10

CPC classification number: H01F1/44 ,  C08F257/00 ,  C08K9/10 ,  C08K2201/01 ,  H01F1/0054 ,  C08F212/36 ,  C08F212/08 ,  C08F2/44

Abstract: Disclosed is a simplified process for producing magnetic polymer particles. The process comprises: (a) providing a composition having a liquid monomer which is radical polymerizable, a radical initiator soluble in the monomer, a steric stabilizer, and a ferrofluid comprising surfactant-coated colloidal magnetic particles in a carrier fluid which is miscible with the monomer; (b) preparing an emulsion from a polar solvent which is immiscible with the monomer, and the composition of step (a); (c) adding seed polymer particles to the emulsion, mixing to form a seeded emulsion, and incubating the seeded emulsion, thereby swelling the seed polymer particles; and (d) activating the radical initiator and polymerizing the monomer in the swollen seed polymer particles; thereby producing the magnetic polymer particles. The process forms monodisperse magnetic particles. The particles are characterized by a uniform distribution of magnetic material, and an absence of magnetite bleeding.

Abstract translation: 公开了用于制备磁性聚合物颗粒的简化方法。 该方法包括：（a）提供具有可自由基聚合的液体单体的组合物，可溶于单体的自由基引发剂，空间稳定剂和包含表面活性剂涂覆的胶体磁性颗粒在载体流体中的铁磁流体，该载体流体可与 单体; （b）从与单体不混溶的极性溶剂和步骤（a）的组成制备乳液; （c）将种子聚合物颗粒加入到乳液中，混合形成接种的乳液，并孵育接种的乳液，从而使种子聚合物颗粒溶胀; 和（d）活化自由基引发剂并使单体在溶胀的种子聚合物颗粒中聚合; 从而产生磁性聚合物颗粒。 该过程形成单分散磁性颗粒。 颗粒的特征在于磁性材料的分布均匀，并且不存在磁铁矿渗出。

公开(公告)号：US20160274137A1

公开(公告)日：2016-09-22

申请号：US15056265

申请日：2016-02-29

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Jonathan Baer

IPC: G01N35/04 ,  B65G43/00 ,  G01N35/00 ,  B65G54/02

CPC classification number: G01N35/04 ,  B65G43/00 ,  B65G54/02 ,  G01N35/00871 ,  G01N2035/046 ,  G01N2035/0477

Abstract: A transport carrier for a laboratory cargo distribution system with a transport plane is disclosed. The transport carrier can be rotationally moved in an easily controllable manner on the transport plane. A laboratory cargo distribution system with a transport plane comprising such a transport carrier and a laboratory automation system comprising such a laboratory cargo distribution system are also disclosed.

Abstract translation: 公开了一种具有运输平面的实验室货物分配系统的运输承运人。 运输托架可以在运输平面上以易于控制的方式旋转移动。 还公开了一种具有包括这种运输工具的运输平面的实验室货物分配系统和包括这种实验室货物分配系统的实验室自动化系统。

公开(公告)号：US20160263576A1

公开(公告)日：2016-09-15

申请号：US15163039

申请日：2016-05-24

Applicant: Roche Diagnostics Operations, Inc. ,  Hitachi High-Technologies Corporation

Inventor： Stephan Sattler ,  Reinhold Kraemer ,  Hidenobu Komatsu ,  Toshio Suzuki

IPC: B01L3/00 ,  G01N35/00

CPC classification number: B01L3/523 ,  B01L3/527 ,  B01L9/00 ,  B01L2200/023 ,  B01L2200/025 ,  B01L2200/16 ,  B01L2300/044 ,  B01L2300/045 ,  B01L2300/0672 ,  G01N35/00663 ,  G01N35/1002 ,  G01N35/1079 ,  G01N2035/00673

Abstract: A reagent vessel holder for an analytical instrument is disclosed. The reagent vessel holder comprises at least one reagent vessel compartment for receiving a reagent vessel, wherein the reagent vessel compartment comprises a base and at least two lateral guiding elements, and at least one hollow needle for piercing the reagent vessel. The hollow needle is disposed on a front end of the reagent vessel compartment near the base and extends in a direction substantially parallel to the base. The lateral guiding elements are disposed so as to be adapted to slidably guide the reagent vessel in a direction substantially parallel to the direction, in which the hollow needle extends. The lateral guiding elements are adapted to receive the reagent vessel therebetween and are biased towards one another. Further, a reagent supply system for an analytical instrument and an analytical instrument comprising such a reagent vessel holder are disclosed.

Abstract translation: 公开了一种用于分析仪器的试剂容器支架。 试剂容器支架包括用于接收试剂容器的至少一个试剂容器隔室，其中试剂容器隔室包括基座和至少两个横向引导元件，以及用于刺穿试剂容器的至少一个空心针。 空心针位于靠近基部的试剂容器隔室的前端，并且在与基座大致平行的方向上延伸。 横向引导元件设置成适于沿着与中空针延伸的方向基本平行的方向可滑动地引导试剂容器。 横向引导元件适于在其间接收试剂容器并且朝向彼此偏置。 此外，公开了一种用于分析仪器的试剂供应系统和包括这种试剂容器保持器的分析仪器。

公开(公告)号：US09423410B2

公开(公告)日：2016-08-23

申请号：US14619332

申请日：2015-02-11

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Fritz Buehr

IPC: G01N21/00 ,  G01N35/04

CPC classification number: G01N35/04 ,  B01L9/06 ,  B01L2200/023 ,  G01N2035/0401 ,  G01N2035/0477

Abstract: A transport device for transporting a sample container along a transport surface is presented. The transport device comprises a permanent magnet, a holding unit and a receiving unit. This enables reliable transporting of sample containers by means of magnetic forces. A sample distribution system and a laboratory automation system are also presented.

Abstract translation: 提出了用于沿着输送面输送试样容器的输送装置。 输送装置包括永磁体，保持单元和接收单元。 这样可以通过磁力可靠地运送样品容器。 还提供了样本分配系统和实验室自动化系统。

公开(公告)号：US20160229565A1

公开(公告)日：2016-08-11

申请号：US15016935

申请日：2016-02-05

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Lech Margner

IPC: B65B7/28 ,  G01N35/02 ,  B65B7/16

CPC classification number: B65B7/28 ,  B65B7/161 ,  G01N35/0099 ,  G01N35/026 ,  G01N35/04 ,  G01N2035/0405

Abstract: A method and a device for handling test tubes in a laboratory automation system are presented. During the handling, an open end of the test tube is covered by a residue-free removable, flat cover element forced against the open end of the test tube.

Abstract translation: 提出了一种在实验室自动化系统中处理试管的方法和装置。 在处理过程中，试管的开口端被一个强制靠在试管开口端的无残留物的可移除的平面盖元件覆盖。

公开(公告)号：US20160216289A1

公开(公告)日：2016-07-28

申请号：US15092806

申请日：2016-04-07

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Manfred Augstein ,  Nadine Losleben ,  Thorsten Brueckner ,  Christoph Boehm ,  Juergen Spinke

IPC: G01N35/10 ,  B01L3/00 ,  G01N35/00

CPC classification number: G01N35/1002 ,  B01L3/021 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L2200/027 ,  B01L2200/04 ,  B01L2300/02 ,  B01L2300/042 ,  B01L2300/0609 ,  B01L2300/0663 ,  B01L2300/123 ,  B01L2400/0481 ,  G01F11/082 ,  G01F11/086 ,  G01F11/088 ,  G01N35/00871 ,  G01N2035/1034

Abstract: A method of performing a measurement of an analyte in a sample using an automatic analyzer is provided. The automatic analyzer comprises: a cartridge for dispensing a fluid, a measurement unit for performing the measurement, a sample holder for receiving the sample, and a pump for pumping the fluid out of the cartridge and into the sample holder. The cartridge comprises: a rigid portion, a flexible bladder, and an outlet. The rigid portion comprises an opening, which is connected to an inner cavity. The flexible bladder seals the opening to form a fluid chamber from the inner cavity. The fluid chamber is at least partially filled with the fluid. The pump is connected to the outlet. The method comprises: placing the sample into the sample holder, controlling the pumping of the fluid from the cartridge into the sample holder, and performing the measurement of the analyte using the measurement unit.

Abstract translation: 提供了使用自动分析仪对样品中的分析物进行测量的方法。 自动分析仪包括：用于分配流体的盒，用于执行测量的测量单元，用于接收样品的样品保持器和用于将流体从盒中抽出并进入样品架的泵。 盒包括：刚性部分，柔性囊和出口。 刚性部分包括连接到内腔的开口。 柔性气囊密封开口以从内腔形成流体室。 流体室至少部分地被流体填充。 泵连接到出口。 该方法包括：将样品放入样品架中，控制将流体从料筒泵送到样品架中，并使用测量单元进行分析物的测量。

公开(公告)号：US20160194410A1

公开(公告)日：2016-07-07

申请号：US15079897

申请日：2016-03-24

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Andreas Gallusser ,  Dieter Heindl ,  Michael Schraeml ,  Christoph Seidel ,  Herbert von der Eltz

IPC: C07K19/00

CPC classification number: C07K19/00 ,  C07K16/18 ,  C07K16/2863 ,  C07K16/32 ,  C07K2317/31 ,  C07K2317/34 ,  C07K2317/55 ,  C07K2317/626 ,  C07K2317/92 ,  C07K2317/94

Abstract: A binding agent of the Formula A-a′:a-S-b:b′-B:X(n), wherein A as well as B is a monovalent binder, a′:a as well as b:b′ is a binding pair wherein a′ and a do not interfere with the binding of b to b′ and vice versa, S is a spacer of at least 1 nm in length, :X denotes a functional moiety bound either covalently or via a binding pair to at least one of a′, a, b, b′ or S, (n) is an integer and at least 1, — represents a covalent bond, and the linker a-S-b has a length of 6 to 100 nm. Also disclosed are methods of producing such binding agent and certain uses thereof.

Abstract translation: 式Aa'的结合剂：aSb：b'-B：X（n），其中A和B是一价结合剂，a：a以及b：b'是一个结合对，其中a' 并且a不干扰b与b'的结合，反之亦然，S是长度至少为1nm的间隔物：X表示共价或通过结合对结合至至少一个“ ，a，b，b'或S，（n）为整数且至少为1， - 表示共价键，连接体aSb的长度为6〜100nm。 还公开了生产这种结合剂及其某些用途的方法。