公开(公告)号：US20120261007A1

公开(公告)日：2012-10-18

申请号：US13295028

申请日：2011-11-11

Applicant: Martin Pieprzyk ,  Geoff Facer ,  Timothy Woudenberg ,  Brian Fowler

Inventor： Martin Pieprzyk ,  Geoff Facer ,  Timothy Woudenberg ,  Brian Fowler

IPC: F16K31/12

CPC classification number: G01N1/28 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/16 ,  B01L2300/0867 ,  B01L2300/0874 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0655 ,  B33Y80/00 ,  C12Q1/686 ,  G01N21/05 ,  G01N21/6452 ,  G01N2021/0346 ,  Y10T137/7793 ,  Y10T436/2575

Abstract: Embodiments of the present invention provide improved microfluidic devices and related apparatus, systems, and methods. Methods are provided for reducing mixing times during use of microfluidic devices. Microfluidic devices and related methods of manufacturing are provided with increased manufacturing yield rates. Improved apparatus and related systems are provided for supplying controlled pressure to microfluidic devices. Methods and related microfluidic devices are provided for reducing dehydration of microfluidic devices during use. Microfluidic devices and related methods are provided with improved sample to reagent mixture ratio control. Microfluidic devices and systems are provided with improved resistance to compression fixture pressure induced failures. Methods and systems for conducting temperature controlled reactions using microfluidic devices are provided that reduce condensation levels within the microfluidic device. Methods and systems are provided for improved fluorescent imaging of microfluidic devices.

Abstract translation: 本发明的实施例提供了改进的微流体装置和相关装置，系统和方法。 提供了减少使用微流体装置期间的混合时间的方法。 微流控装置和相关的制造方法具有提高的制造成品率。 提供改进的装置和相关系统用于向微流体装置提供受控的压力。 提供了方法和相关的微流体装置，用于在使用过程中减少微流体装置的脱水。 微流控装置和相关方法提供了改进的样品与试剂混合比控制。 微流体装置和系统具有改进的抗压缩夹具压力引起的故障的阻力。 提供了使用微流体装置进行温度控制反应的方法和系统，其减少微流体装置内的冷凝水平。 提供了用于改进微流体装置的荧光成像的方法和系统。

公开(公告)号：US20120045087A1

公开(公告)日：2012-02-23

申请号：US13022856

申请日：2011-02-08

Applicant: Gang Sun ,  Greg Harris ,  Andy May ,  Kyle Self ,  Kevin Farrell ,  Paul Wyatt

Inventor： Gang Sun ,  Greg Harris ,  Andy May ,  Kyle Self ,  Kevin Farrell ,  Paul Wyatt

IPC: G06K9/00

CPC classification number: G01N35/00029 ,  B01L3/5027 ,  B01L3/502715 ,  B01L2200/10 ,  B01L2300/06 ,  B01L2300/0654 ,  B01L2300/0829 ,  C12Q1/686 ,  G01N15/1475 ,  G01N21/6428 ,  G01N35/00732 ,  G01N35/00871 ,  G01N35/0092 ,  G01N35/0099 ,  G01N35/1065 ,  G01N2035/00148 ,  G01N2035/00237 ,  G01N2035/00752 ,  G01N2035/0091 ,  G01N2035/1034 ,  G01N2201/025 ,  G01N2201/0446 ,  Y10S901/01 ,  Y10T436/11

Abstract: Systems for managing workflows to perform chemical or biological reactions using microfluidic devices.

Abstract translation: 用于管理使用微流体装置进行化学或生物反应的工作流的系统。

公开(公告)号：US08105550B2

公开(公告)日：2012-01-31

申请号：US12645396

申请日：2009-12-22

Applicant: Emerson Chueng Quan ,  Colin Jon Taylor ,  Michael Lee ,  Christopher G. Cesar ,  Greg Harris ,  Gang Sun

Inventor： Emerson Chueng Quan ,  Colin Jon Taylor ,  Michael Lee ,  Christopher G. Cesar ,  Greg Harris ,  Gang Sun

IPC: G01N33/00

CPC classification number: G01N33/00 ,  B01L3/5025 ,  B01L3/502707 ,  B01L2200/025 ,  B01L2200/12 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/0887 ,  B01L2400/0481 ,  B01L2400/0655 ,  Y10T436/11

Abstract: A biological substrate, e.g., microfluidic chip. The substrate includes a rigid substrate material, which has a surface region capable of acting as a handle substrate. The substrate also has a deformable fluid layer coupled to the surface region. One or more well regions are formed in a first portion of the deformable fluid layer and are capable of holding a fluid therein. The one or more channel regions are formed in a second portion of the deformable fluid layer and are coupled to one or more of the well regions. An active region is formed in the deformable fluid layer. At least three fiducial markings are formed within the non-active region and disposed in a spatial manner associated with at least one of the well regions. A control layer is coupled to the fluid layer.

Abstract translation: 生物底物，例如微流体芯片。 衬底包括刚性衬底材料，其具有能够用作手柄衬底的表面区域。 衬底还具有耦合到表面区域的可变形流体层。 一个或多个阱区域形成在可变形流体层的第一部分中并且能够在其中保持流体。 一个或多个通道区域形成在可变形流体层的第二部分中并且耦合到一个或多个阱区域。 在可变形流体层中形成有源区。 在非有源区域内形成至少三个基准标记，并以与至少一个阱区域相关联的空间方式设置。 控制层耦合到流体层。

公开(公告)号：US08055034B2

公开(公告)日：2011-11-08

申请号：US11900927

申请日：2007-09-13

Applicant: Simant Dube ,  Gang Sun ,  Lian-She Zhao

Inventor： Simant Dube ,  Gang Sun ,  Lian-She Zhao

IPC: G06K9/36

CPC classification number: G01N21/64 ,  G06T7/0012 ,  G06T7/74 ,  G06T2207/10064 ,  G06T2207/30004

Abstract: A method of processing data associated with fluorescent emissions from a microfluidic device. The method includes performing an auto-focus process associated with a first image of the microfluidic device and performing an auto-exposure process associated with the first image of the microfluidic device. The method also includes capturing a plurality of images of the microfluidic device. The plurality of images are associated with a plurality of thermal cycles. The method further includes performing image analysis of the plurality of captured images to determine a series of optical intensities and performing data analysis of the series of optical intensities to provide a series of change in threshold values.

Abstract translation: 处理与微流体装置的荧光发射有关的数据的方法。 该方法包括执行与微流体装置的第一图像相关联的自动聚焦处理，并且执行与微流体装置的第一图像相关联的自动曝光处理。 该方法还包括捕获微流体装置的多个图像。 多个图像与多个热循环相关联。 该方法还包括执行多个拍摄图像的图像分析以确定一系列光强度并执行该系列光强度的数据分析以提供一系列阈值变化。

公开(公告)号：US08050516B2

公开(公告)日：2011-11-01

申请号：US11900926

申请日：2007-09-13

Applicant: Simant Dube ,  Gang Sun ,  Lian-She Zhao

Inventor： Simant Dube ,  Gang Sun ,  Lian-She Zhao

IPC: G06K9/36

CPC classification number: G06K9/00134

Abstract: A method of determining a plurality of positions associated with a plurality of reaction chambers of a microfluidic device includes a) providing a baseline image; b) providing a template image of a reaction chamber; and c) selecting a region of the baseline image. The method also includes d) performing a matching process including matching the template image to one or more portions of the region of the baseline image; e) determining a position of a first chamber; and f) predicting a position of a second chamber. The method further includes g) repeating steps c) through f) for subsequent chambers.

Abstract translation: 确定与微流体装置的多个反应室相关联的多个位置的方法包括：a）提供基线图像; b）提供反应室的模板图像; 和c）选择基线图像的区域。 该方法还包括：d）执行匹配过程，包括将模板图像与基线图像区域的一个或多个部分相匹配; e）确定第一室的位置; 以及f）预测第二室的位置。 该方法还包括g）重复步骤c）至f）以用于随后的室。

公开(公告)号：US20110189678A1

公开(公告)日：2011-08-04

申请号：US12859176

申请日：2010-08-18

Applicant: Lincoln McBride ,  Michael Lucero ,  Marc Unger ,  Hany Ramez Nassef ,  Geoffrey Facer

Inventor： Lincoln McBride ,  Michael Lucero ,  Marc Unger ,  Hany Ramez Nassef ,  Geoffrey Facer

IPC: C12Q1/68 ,  B01L3/00 ,  G01N1/28

CPC classification number: B01L3/00 ,  B01F13/0059 ,  B01J2219/00337 ,  B01J2219/00353 ,  B01J2219/00403 ,  B01J2219/00495 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/0072 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/502738 ,  B01L3/50851 ,  B01L2300/0627 ,  B01L2300/0883 ,  B01L2300/123 ,  B01L2400/06 ,  C12Q1/6844 ,  C40B60/08 ,  C40B60/14 ,  G01N35/00029 ,  G01N35/0098 ,  G01N2035/00366 ,  Y10T436/25 ,  C12Q2565/501

Abstract: A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyses.

公开(公告)号：US20110166044A1

公开(公告)日：2011-07-07

申请号：US12872498

申请日：2010-08-31

Applicant: Robert C. Jones ,  Paul Wyatt ,  Antoine Daridon ,  Jing Wang ,  Andrew May ,  David Cohen

Inventor： Robert C. Jones ,  Paul Wyatt ,  Antoine Daridon ,  Jing Wang ,  Andrew May ,  David Cohen

IPC: C40B60/08

CPC classification number: B01J19/00 ,  B01F5/0085 ,  B01F5/0602 ,  B01F13/0069 ,  B01J2219/00283 ,  B01J2219/00286 ,  B01J2219/00315 ,  B01J2219/00317 ,  B01J2219/00351 ,  B01J2219/00355 ,  B01J2219/00389 ,  B01J2219/00418 ,  B01L3/502715 ,  B01L3/502738 ,  B01L3/5085 ,  B01L7/52 ,  B01L2200/16 ,  B01L2300/0654 ,  B01L2300/0829 ,  B01L2300/123 ,  B01L2300/1805 ,  B01L2400/0481 ,  B01L2400/0605 ,  B01L2400/0655

Abstract: An SBS-formatted microfluidic device where the geometry of the plate defines an array of interrogation areas, and where each interrogation area encompasses at least one reaction site.

Abstract translation: SBS格式的微流体装置，其中板的几何形状限定了询问区域阵列，并且其中每个询问区域包含至少一个反应位点。

公开(公告)号：US20100320364A1

公开(公告)日：2010-12-23

申请号：US12862621

申请日：2010-08-24

Applicant: Marc A. Unger ,  Geoffrey Richard Facer ,  Barry Clerksen ,  Christopher G. Cesar ,  Neil Switz

Inventor： Marc A. Unger ,  Geoffrey Richard Facer ,  Barry Clerksen ,  Christopher G. Cesar ,  Neil Switz

IPC: H01L27/148 ,  G01N21/64

CPC classification number: C12Q1/686 ,  B01J2219/00576 ,  B01J2219/00704 ,  B01L3/5027 ,  B01L7/52 ,  G01N21/64 ,  G01N21/6452 ,  G01N21/6456 ,  G01N21/6486 ,  G01N21/75 ,  G01N21/8483 ,  G01N27/44721 ,  G01N2021/6421 ,  G01N2201/061 ,  G02B21/16 ,  G02B21/36

Abstract: An apparatus for imaging one or more selected fluorescence indications from a microfluidic device. The apparatus includes an imaging path coupled to least one chamber in at least one microfluidic device. The imaging path provides for transmission of one or more fluorescent emission signals derived from one or more samples in the at least one chamber of the at least one microfluidic device. The chamber has a chamber size, the chamber size being characterized by an actual spatial dimension normal to the imaging path. The apparatus also includes an optical lens system coupled to the imaging path. The optical lens system is adapted to transmit the one or more fluorescent signals associated with the chamber.

Abstract translation: 一种用于从微流体装置成像一个或多个选定的荧光指示的装置。 该装置包括耦合到至少一个微流体装置中的至少一个室的成像路径。 成像路径提供从至少一个微流体装置的至少一个室中的一个或多个样品衍生的一个或多个荧光发射信号的传输。 腔室具有腔室尺寸，腔室尺寸的特征在于与成像路径垂直的实际空间尺寸。 该装置还包括耦合到成像路径的光学透镜系统。 光学透镜系统适于透射与腔室相关联的一个或多个荧光信号。

公开(公告)号：US20100263757A1

公开(公告)日：2010-10-21

申请号：US12621108

申请日：2009-11-18

Applicant: David Fernandes ,  Hou-Pu Chou ,  Marc A. Unger

Inventor： David Fernandes ,  Hou-Pu Chou ,  Marc A. Unger

IPC: F15C3/00 ,  F16K15/14

CPC classification number: F15C3/00 ,  A61M2206/22 ,  B01L3/502707 ,  B01L3/50273 ,  B01L3/502738 ,  B01L2200/0621 ,  B01L2200/14 ,  B01L2300/123 ,  B01L2400/0487 ,  B01L2400/0638 ,  B01L2400/0655 ,  F15C3/04 ,  F16K99/0001 ,  F16K99/0009 ,  F16K99/0015 ,  F16K99/0034 ,  F16K99/0059 ,  F16K2099/0074 ,  F16K2099/008 ,  F16K2099/0082 ,  F16K2099/0084 ,  G06F17/509 ,  G06F2217/16 ,  H01H2029/008 ,  Y10T137/206 ,  Y10T137/2202 ,  Y10T137/2218 ,  Y10T137/2224 ,  Y10T137/7879 ,  Y10T137/7891 ,  Y10T137/7892

Abstract: A microfabricated fluidic unidirectional valve includes a microfabricated elastomer material having a flow through channel. The microfabricated fluidic unidirectional valve also includes an elastomer flap attached to the elastomer material in the flow through channel. The elastomer flap forms a seal in the flow through channel to prevent fluid from flowing in a first direction through the flow through channel and to allow fluid flow in a second direction through the flow through channel.

Abstract translation: 微制造的流体单向阀包括具有流通通道的微加工弹性体材料。 微制造的流体单向阀还包括在流动通道中连接到弹性体材料的弹性体翼片。 弹性翼片在流通通道中形成密封，以防止流体沿第一方向流过通过通道并允许流体沿第二方向流过通过通道的流体。

公开(公告)号：US20100230613A1

公开(公告)日：2010-09-16

申请号：US12688462

申请日：2010-01-15

Applicant: Martin Pieprzyk ,  Geoff Facer ,  Timothy Woudenberg ,  Brian Fowler

Inventor： Martin Pieprzyk ,  Geoff Facer ,  Timothy Woudenberg ,  Brian Fowler

IPC: G01J1/58

CPC classification number: G01N1/28 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/16 ,  B01L2300/0867 ,  B01L2300/0874 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0655 ,  B33Y80/00 ,  C12Q1/686 ,  G01N21/05 ,  G01N21/6452 ,  G01N2021/0346 ,  Y10T137/7793 ,  Y10T436/2575

Abstract: Embodiments of the present invention provide improved microfluidic devices and related apparatus, systems, and methods. Methods are provided for reducing mixing times during use of microfluidic devices. Microfluidic devices and related methods of manufacturing are provided with increased manufacturing yield rates. Improved apparatus and related systems are provided for supplying controlled pressure to microfluidic devices. Methods and related microfluidic devices are provided for reducing dehydration of microfluidic devices during use. Microfluidic devices and related methods are provided with improved sample to reagent mixture ratio control. Microfluidic devices and systems are provided with improved resistance to compression fixture pressure induced failures. Methods and systems for conducting temperature controlled reactions using microfluidic devices are provided that reduce condensation levels within the microfluidic device. Methods and systems are provided for improved fluorescent imaging of microfluidic devices.

Abstract translation: 本发明的实施例提供了改进的微流体装置和相关装置，系统和方法。 提供了减少使用微流体装置期间的混合时间的方法。 微流控装置和相关的制造方法具有提高的制造产率。 提供改进的装置和相关系统用于向微流体装置提供受控的压力。 提供了方法和相关的微流体装置，用于在使用过程中减少微流体装置的脱水。 微流控装置和相关方法提供了改进的样品与试剂混合比控制。 微流体装置和系统具有改进的抗压缩夹具压力引起的故障的阻力。 提供了使用微流体装置进行温度控制反应的方法和系统，其减少微流体装置内的冷凝水平。 提供了用于改进微流体装置的荧光成像的方法和系统。