公开(公告)号：US20170058334A1

公开(公告)日：2017-03-02

申请号：US15096114

申请日：2016-04-11

Applicant: Clondiag Chip Technologies GmbH

Inventor： Torsten Schulz ,  Eugen Ermantraut ,  Ralf Ehricht ,  Klaus- Peter Mobius ,  Gerd Wagner ,  Joachim Fischer ,  Thomas Ellinger

IPC: C12Q1/68

CPC classification number: C12Q1/6837 ,  B01J2219/00283 ,  B01J2219/00529 ,  B01J2219/0054 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00707 ,  B01J2219/00722 ,  B01L3/50 ,  B01L3/5082 ,  B01L7/52 ,  B01L2300/0636 ,  B01L2300/0851 ,  C40B40/06 ,  C40B60/14 ,  C40B70/00 ,  G01N21/253 ,  G01N33/54366 ,  C12Q2547/101

Abstract: The invention relates to a reaction vessel, a device and a method for detecting specific interactions between molecular target and probe molecules. The present invention especially relates to a reaction vessel which has a shape and size typical of a laboratory reaction vessel and in which a supporting element with probe molecules immobilised thereon on predetermined regions is arranged on its base surfaces.

Abstract translation: 本发明涉及一种用于检测分子靶和探针分子之间的特异性相互作用的反应容器，装置和方法。 本发明特别涉及一种反应容器，其具有典型的实验室反应容器的形状和尺寸，其中在预定区域上固定有探针分子的支撑元件布置在其基面上。

公开(公告)号：US20170021325A1

公开(公告)日：2017-01-26

申请号：US15287077

申请日：2016-10-06

Applicant: Illumina Cambridge Limited

Inventor： Xiaohai Liu ,  John Milton ,  Geoffrey Paul Smith ,  Colin Lloyd Barnes ,  Isabelle Rasolonjatovo ,  Roberto Rigatti ,  Xiaolin Wu ,  Tobias William Barr Ost ,  Graham John Worsley ,  David James Earnshaw ,  Gerardo Turcatti ,  Anthony Romieu

IPC: B01J19/00 ,  C12Q1/68

CPC classification number: B01J19/0046 ,  B01J2219/00596 ,  B01J2219/00608 ,  B01J2219/00675 ,  B01J2219/00722 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6874 ,  C12Q2565/543 ,  C12Q2535/101 ,  C12Q2523/113 ,  C12Q2523/107 ,  C12Q2521/331 ,  C12Q2521/307

Abstract: The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to a solid support at the 5′ end, cleaving one or both strands of the double-stranded nucleic acid molecule, and subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction.

Abstract translation: 本发明涉及产生用于核酸测序反应的模板的方法，其包括：提供至少一个双链核酸分子，其中双链核酸分子的两条链在5'末端连接到固体支持物上 切割双链核酸分子的一条或两条链，并使切割的链进行变性条件以除去未连接到固体支持物上的部分切割的链，从而产生部分或基本上 用于核酸测序反应的单链模板。

公开(公告)号：US20170017436A1

公开(公告)日：2017-01-19

申请号：US15209127

申请日：2016-07-13

Applicant: President and Fellows of Harvard College

Inventor： George M. Church

IPC: G06F3/06 ,  B01J19/00 ,  G11B7/245 ,  C12Q1/68

CPC classification number: G06F19/18 ,  B01J19/0046 ,  B01J2219/00529 ,  B01J2219/00547 ,  B01J2219/00572 ,  B01J2219/00596 ,  B01J2219/00608 ,  B01J2219/00675 ,  B01J2219/00711 ,  B01J2219/00713 ,  B01J2219/00722 ,  C12Q1/68 ,  C12Q1/6874 ,  G06F5/08 ,  G06F19/00 ,  G11B7/241 ,  G11B7/245 ,  G11C7/00 ,  C12Q2563/179 ,  C12Q2563/185

Abstract: A method of storing information using monomers such as nucleotides is provided including converting a format of information into a plurality of bit sequences of a bit stream with each having a corresponding bit barcode, converting the plurality of bit sequences to a plurality of corresponding oligonucleotide sequences using one bit per base encoding, synthesizing the plurality of corresponding oligonucleotide sequences on a substrate having a plurality of reaction locations, and storing the synthesized plurality of corresponding oligonucleotide sequences.

Abstract translation: 提供了使用诸如核苷酸的单体存储信息的方法，包括将信息格式转换成具有相应位条形码的位流的多个比特序列，将多个比特序列转换成多个相应寡核苷酸序列，使用 每个碱基编码一位，在具有多个反应位置的底物上合成多个相应的寡核苷酸序列，并存储合成的多个相应的寡核苷酸序列。

公开(公告)号：US20170007977A1

公开(公告)日：2017-01-12

申请号：US15203582

申请日：2016-07-06

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Alexander MASTROIANNI ,  Scott C. BENSON ,  Steven M. MENCHEN ,  John SCOTT

IPC: B01J19/00

CPC classification number: B01J19/0046 ,  B01J2219/00317 ,  B01J2219/005 ,  B01J2219/00545 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00722 ,  C12Q1/6806 ,  C12Q1/6837 ,  C12Q1/6869 ,  C12Q2535/122 ,  C12Q2563/155 ,  C12Q2565/607 ,  C12Q2565/629

Abstract: A method of preparing a discrete polymer network array include mixing a plurality of nucleic acid polymer networks with a plurality of color-activated polymer networks to form a dispersion, applying the dispersion to an array of wells, the nucleic acid polymer networks selectively depositing into wells of the array of wells, and rinsing the array of wells to selectively remove the plurality of color-activated polymer networks.

Abstract translation: 制备离散聚合物网络阵列的方法包括将多个核酸聚合物网络与多个着色活化聚合物网络混合以形成分散体，将该分散体施加到孔阵列上，将核酸聚合物网络选择性地沉积到孔中 的孔阵列，并且冲洗孔阵列以选择性地去除多个着色活化聚合物网络。

公开(公告)号：US09534250B2

公开(公告)日：2017-01-03

申请号：US13095790

申请日：2011-04-27

Applicant: David Van Steenwinckel ,  Filip Frederix

Inventor： David Van Steenwinckel ,  Filip Frederix

IPC: C12Q1/68 ,  C40B50/18 ,  C40B60/12 ,  C40B50/14

CPC classification number: C12Q1/6837 ,  B01J2219/00529 ,  B01J2219/00576 ,  B01J2219/00608 ,  B01J2219/00653 ,  B01J2219/00722 ,  C40B50/14 ,  C40B50/18 ,  C40B60/12 ,  C12Q2565/518 ,  C12Q2565/607 ,  C12Q2525/197

Abstract: The present invention relates to a sensing device with a surface having at least one individual sensing region, wherein each sensing region includes a plurality of binding elements anchored on the surface for binding different specific analytes of interest, at least one of the analyte of interest and its matching binding element having a label for detecting said binding. The present invention further relates to a method of manufacturing such a sensing device.

Abstract translation: 本发明涉及具有表面的感测装置，其具有至少一个单独的感测区域，其中每个感测区域包括锚定在表面上的多个结合元件，用于结合感兴趣的不同特定分析物，感兴趣的分析物中的至少一种和 其匹配绑定元件具有用于检测所述绑定的标签。 本发明还涉及一种制造这种感测装置的方法。

公开(公告)号：US20160362733A1

公开(公告)日：2016-12-15

申请号：US15188173

申请日：2016-06-21

Applicant: ILLUMINA, INC.

Inventor： Mark S. Chee ,  John R. Stuelpnagel ,  Anthony W. Czarnik

IPC: C12Q1/68 ,  G06K9/62

CPC classification number: C12Q1/6837 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00459 ,  B01J2219/00466 ,  B01J2219/005 ,  B01J2219/00572 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00619 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00644 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00702 ,  B01J2219/00722 ,  B01L3/5085 ,  B82Y30/00 ,  C12Q1/6816 ,  C12Q1/6825 ,  C12Q1/6834 ,  C40B40/06 ,  C40B60/14 ,  C40B70/00 ,  G01N33/54313 ,  G06K9/6202 ,  Y10S977/924 ,  C12Q2563/155 ,  C12Q2563/179 ,  C12Q2563/107 ,  C12Q2527/137 ,  C12Q2521/313 ,  C12Q2537/143

Abstract: The invention relates to compositions and methods for multiplex decoding of microsphere array sensors.

公开(公告)号：US09512422B2

公开(公告)日：2016-12-06

申请号：US13787396

申请日：2013-03-06

Applicant: Illumina, Inc.

Inventor： Steven M. Barnard ,  M. Shane Bowen ,  Maria Candelaria Rogert Bacigalupo ,  Wayne N. George ,  Andrew A. Brown ,  James Tsay

IPC: C12N15/10 ,  C12Q1/68 ,  B01J19/00

CPC classification number: B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00621 ,  B01J2219/00644 ,  B01J2219/00722 ,  C12N15/1068 ,  C12Q1/6837 ,  C12Q2563/159

Abstract: Provided is an array including a solid support having a surface, the surface having a plurality of wells, the wells containing a gel material, the wells being separated from each other by interstitial regions on the surface, the interstitial regions segregating the gel material in each of the wells from the gel material in other wells of the plurality; and a library of target nucleic acids in the gel material, wherein the gel material in each of the wells comprises a single species of the target nucleic acids of the library. Methods for making and using the array are also provided.

Abstract translation: 提供了一种阵列，其包括具有表面的固体支持物，所述表面具有多个孔，所述孔包含凝胶材料，所述孔通过表面上的间隙区彼此分离，所述间隙区域在每个孔中分离凝胶材料 的来自多个其他孔中的凝胶材料的孔; 和凝胶材料中的靶核酸文库，其中每个孔中的凝胶材料包含文库的单个靶核酸的种类。 还提供了制作和使用阵列的方法。

公开(公告)号：US20160339439A1

公开(公告)日：2016-11-24

申请号：US15159026

申请日：2016-05-19

Applicant: Mitsubishi Rayon Co., Ltd.

Inventor： Kouji SHIMIZU

IPC: B01L9/00 ,  B01J19/00

CPC classification number: B01L9/523 ,  B01J19/0046 ,  B01J2219/00313 ,  B01J2219/00317 ,  B01J2219/00373 ,  B01J2219/00414 ,  B01J2219/00533 ,  B01J2219/00585 ,  B01J2219/00716 ,  B01J2219/00722 ,  B01L2200/025 ,  B01L2300/0829 ,  G01N35/028 ,  G01N35/1011 ,  G01N2035/0437

Abstract: Provided is a microarray processing apparatus which is capable of sufficiently washing a microarray. The microarray processing apparatus (30) includes a well plate (38) in which one or two or more wells (40) are formed, each well (40) accommodating a microarray (1), and a suction nozzle (46) that suctions a liquid from the well. The well has a concave portion whose upper end is opened, which has a depth equal to or greater than a height of the microarray, and into which a front end of the suction nozzle can be inserted up to a height position of a lower end of the microarray accommodated in the well. The suction nozzle can relatively descend in the well until the front end of the suction nozzle is located at the height position of the lower end of the microarray accommodated in the well.

Abstract translation: 提供一种能够充分洗涤微阵列的微阵列处理装置。 微阵列处理装置（30）包括其中形成有一个或两个以上的孔（40）的孔板（38），容纳微阵列（1）的每个孔（40）和吸入喷嘴 液体从井。 井具有上端开口的凹部，其深度等于或大于微阵列的高度，并且吸嘴的前端可以插入其中，直到下端的高度位置 微阵列容纳在井中。 吸嘴可以在井中相对下降，直到吸嘴的前端位于容纳在井中的微阵列的下端的高度位置。

公开(公告)号：US09499863B2

公开(公告)日：2016-11-22

申请号：US14028319

申请日：2013-09-16

Applicant: Complete Genomics, Inc.

Inventor： Radoje Drmanac ,  Brock A. Peters ,  Andrei Alexeev ,  Peter Hong

IPC: C12Q1/68 ,  C12P19/34

CPC classification number: B01J19/0046 ,  B01J2219/00722 ,  C12P19/34 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2525/101 ,  C12Q2521/101

Abstract: The present invention is directed to methods and compositions for long fragment read sequencing. The present invention encompasses methods and compositions for preparing long fragments of genomic DNA, for processing genomic DNA for long fragment read sequencing methods, as well as software and algorithms for processing and analyzing sequence data.

Abstract translation: 本发明涉及用于长片段读取测序的方法和组合物。 本发明包括用于制备基因组DNA长片段的方法和组合物，用于处理长片段读取测序方法的基因组DNA，以及用于处理和分析序列数据的软件和算法。

公开(公告)号：US09476854B2

公开(公告)日：2016-10-25

申请号：US15040815

申请日：2016-02-10

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kristopher Barbee ,  John F. Davidson ,  Wolfgang Hinz ,  Shifeng Li ,  James Bustillo

IPC: G01N27/414 ,  B01J19/00

CPC classification number: G01N27/4473 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00317 ,  B01J2219/00459 ,  B01J2219/00466 ,  B01J2219/00468 ,  B01J2219/005 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00653 ,  B01J2219/00704 ,  B01J2219/00722 ,  C12Q1/6874 ,  G01N27/4145 ,  G01N27/4148 ,  G01N27/44743

Abstract: An apparatus includes a device substrate including an array of sensors. Each sensor of the array of sensors can include a electrode structure disposed at a surface of the device substrate. The apparatus further includes a wall structure overlying the surface of the device substrate and defining an array of wells at least partially corresponding with the array of sensors. The well structure including an electrode layer and an insulative layer.

Abstract translation: 一种装置包括包括传感器阵列的装置基板。 传感器阵列的每个传感器可以包括设置在器件基板的表面处的电极结构。 该装置还包括覆盖在装置基板的表面上的壁结构，并且限定至少部分对应于传感器阵列的一组阱阵列。 阱结构包括电极层和绝缘层。