公开(公告)号：US20160209320A1

公开(公告)日：2016-07-21

申请号：US14974278

申请日：2015-12-18

Applicant: Roche Diagnostics Hematology, Inc.

Inventor： James Winkelman ,  Milenko Tanasijevic ,  David Zahniser

IPC: G01N15/14 ,  G01N35/10 ,  G01N35/00 ,  G01N1/31 ,  G01N33/49

CPC classification number: G01N15/1475 ,  G01N1/2813 ,  G01N1/312 ,  G01N21/255 ,  G01N33/49 ,  G01N35/00029 ,  G01N35/00871 ,  G01N35/1016 ,  G01N2015/0073 ,  G01N2015/008 ,  G01N2015/0084 ,  G01N2015/1006 ,  G01N2015/1472 ,  G01N2015/1486 ,  G01N2015/1497 ,  G01N2035/00138 ,  G01N2035/00465 ,  G01N2035/0091 ,  G01N2201/062 ,  G01N2201/126 ,  G01N2333/805 ,  G06K9/4652 ,  G06K9/6267 ,  G06T7/0012 ,  H04N5/2256 ,  H04N5/23293 ,  H04N5/265

Abstract: Systems and methods analyzing body fluids such as blood and bone marrow are disclosed. The systems and methods may utilize an improved technique for applying a monolayer of cells to a slide to generate a substantially uniform distribution of cells on the slide. Additionally aspects of the invention also relate to systems and methods for utilizing multi color microscopy for improving the quality of images captured by a light receiving device.

Abstract translation: 公开了分析体液如血液和骨髓的系统和方法。 系统和方法可以利用改进的技术将单层细胞施加到载玻片上以在载玻片上产生基本均匀的细胞分布。 本发明的另外方面还涉及用于利用多色显微镜来提高由光接收装置捕获的图像的质量的系统和方法。

公开(公告)号：US20160169923A1

公开(公告)日：2016-06-16

申请号：US14965725

申请日：2015-12-10

Applicant: Theranos, Inc.

Inventor： Elizabeth A. Holmes ,  Sunny Balwani ,  Michael Chen ,  John K. Frankovich ,  Gary Frenzel ,  Surekha Gangakhedkar ,  Samartha Anekal ,  Adrit Lath ,  Alexander Loo ,  Chinmay Pangarkar ,  Paul Patel ,  Joy Roy ,  Timothy Smith ,  Daniel Young ,  Ian Gibbons

IPC: G01N35/00 ,  B01L3/02 ,  B01L3/00 ,  G01N35/10 ,  B01L7/00

CPC classification number: G01N35/0092 ,  B01L3/021 ,  B01L3/5082 ,  B01L3/50855 ,  B01L7/52 ,  B01L9/06 ,  B01L9/52 ,  B01L9/543 ,  B01L2300/1827 ,  B01L2300/1855 ,  C12Q1/42 ,  C12Q1/52 ,  C12Q1/6883 ,  C12Q1/70 ,  G01N15/1475 ,  G01N21/25 ,  G01N21/27 ,  G01N33/5005 ,  G01N33/54306 ,  G01N33/54313 ,  G01N33/54366 ,  G01N33/56983 ,  G01N33/62 ,  G01N33/6827 ,  G01N33/80 ,  G01N33/92 ,  G01N35/00 ,  G01N35/00029 ,  G01N35/00069 ,  G01N35/00623 ,  G01N35/00871 ,  G01N35/026 ,  G01N35/04 ,  G01N35/10 ,  G01N35/1009 ,  G01N35/1011 ,  G01N35/1065 ,  G01N35/1072 ,  G01N2015/0073 ,  G01N2015/008 ,  G01N2015/1006 ,  G01N2015/1486 ,  G01N2035/00138 ,  G01N2035/00148 ,  G01N2035/00237 ,  G01N2035/00306 ,  G01N2035/00326 ,  G01N2035/00356 ,  G01N2035/00366 ,  G01N2035/00425 ,  G01N2035/00435 ,  G01N2035/00495 ,  G01N2035/00633 ,  G01N2035/0449 ,  G01N2035/0474 ,  G01N2035/0486 ,  G01N2035/0491 ,  G01N2035/0493 ,  G01N2035/0494 ,  G01N2201/024 ,  G01N2201/04 ,  G01N2201/12 ,  Y10T436/111666 ,  Y10T436/113332

Abstract: Systems and methods are provided for sample processing. A device may be provided, capable of receiving the sample, and performing one or more of a sample preparation, sample assay, and detection step. The device may be capable of performing multiple assays. The device may comprise one or more modules that may be capable of performing one or more of a sample preparation, sample assay, and detection step. The device may be capable of performing the steps using a small volume of sample.

公开(公告)号：US20160161394A1

公开(公告)日：2016-06-09

申请号：US15045691

申请日：2016-02-17

Applicant: FUJIFILM Corporation

Inventor： Kenta MATSUBARA

IPC: G01N15/14 ,  G06T7/20 ,  H04N5/232 ,  H04N5/235 ,  G06T7/00 ,  G06K9/62

CPC classification number: G01N15/1463 ,  C12M41/36 ,  C12Q1/04 ,  G01N15/1475 ,  G01N2015/0088 ,  G01N2015/1006 ,  G01N2015/1488 ,  G01N2015/1493 ,  G01N2015/1497 ,  G06K9/00134 ,  G06K9/00147 ,  G06K9/6201 ,  G06K2009/6213 ,  G06T7/0012 ,  G06T7/20 ,  G06T2207/30024 ,  H04N5/23296 ,  H04N5/2353

Abstract: An observation image determination device includes an observation image acquisition unit that captures an image of an observation region including a stem cell to be cultured to acquire an observation image and a determination unit that determines whether a living body of a different type from the stem cell is included in the observation region. The determination unit determines whether the different type of living body is included in the observation region, on the basis of at least one of form information of an observation target and information about a change in the observation target over time which are acquired from the observation image.

Abstract translation: 观察图像确定装置包括观察图像获取单元，其捕获包括待培养的干细胞的观察区域的图像以获取观察图像;以及确定单元，其确定与干细胞不同类型的生物体是否为 包括在观察区域。 判断单元根据从观察图像获取的观察对象的形态信息和关于观察对象随时间的变化的信息中的至少一个，判定观察区域中是否包含不同类型的生物体 。

公开(公告)号：US09322752B2

公开(公告)日：2016-04-26

申请号：US14216533

申请日：2014-03-17

Applicant: IRIS International, Inc.

Inventor： Bart J. Wanders ,  Thomas H. Adams ,  Gregory A. Farrell ,  Warren Groner ,  Xiaodong Zhao

IPC: G01N33/00 ,  G01N31/00 ,  G01N1/30 ,  G01N33/50 ,  G01N33/80 ,  G01N21/53 ,  G01N15/14 ,  G01N15/00 ,  G01N15/10 ,  G01N21/05

CPC classification number: G01N15/1404 ,  G01N1/30 ,  G01N15/1434 ,  G01N15/1463 ,  G01N15/1468 ,  G01N15/147 ,  G01N15/1475 ,  G01N21/53 ,  G01N33/49 ,  G01N33/4915 ,  G01N33/5091 ,  G01N33/5094 ,  G01N33/80 ,  G01N2015/0065 ,  G01N2015/1037 ,  G01N2015/1411 ,  G01N2015/1413 ,  G01N2015/1452 ,  G01N2015/1486 ,  G01N2021/058

Abstract: The present disclosure relates to apparatus, systems, compositions, and methods for analyzing a sample containing particles. In some aspects the system comprises an analyzer which may be a visual analyzer. In one aspect, this disclosure relates to a particle imaging system comprising a flowcell through which a sample containing particles is caused to flow, and a high optical resolution imaging device which captures images for image analysis of samples. Other compositions, methods and features of this disclosure are disclosed herein.

Abstract translation: 本公开涉及用于分析含有颗粒的样品的装置，系统，组合物和方法。 在一些方面，该系统包括可以是视觉分析仪的分析器。 一方面，本发明涉及一种粒子成像系统，该粒子成像系统包括流过细胞，通过该流通池使含有颗粒的样品流动;以及高光学分辨率成像装置，其捕获用于样品的图像分析的图像。 本公开的其它组合物，方法和特征在此公开。

公开(公告)号：US20160047797A1

公开(公告)日：2016-02-18

申请号：US14673294

申请日：2015-03-30

Applicant: Robert A. Levine ,  Stephen C. Wardlaw ,  Niten V. Lalpuria ,  Darryn W. Unfricht

Inventor： Robert A. Levine ,  Stephen C. Wardlaw ,  Niten V. Lalpuria ,  Darryn W. Unfricht

IPC: G01N33/50 ,  G01N21/64

CPC classification number: G01N33/49 ,  G01N15/1463 ,  G01N15/1475 ,  G01N21/6428 ,  G01N21/6456 ,  G01N33/48 ,  G01N33/5091 ,  G01N2015/008 ,  G01N2015/1477 ,  G01N2021/6432 ,  G01N2021/6439 ,  G01N2201/062

Abstract: A method for analyzing a blood sample is provided that includes the steps of: providing a blood sample having one or more of each first and second constituents; admixing a colorant with the sample, which colorant is operative to cause the first constituents and second constituents to fluoresce and absorb light; illuminating at least a portion of the sample; e) imaging a portion of the sample; determining a fluorescence value for each the first constituents and second constituents; determining an optical density value for each of the first constituents and second constituents; and identifying the first constituents and the second constituents using the determined fluorescence and optical density values.

公开(公告)号：US20160026852A1

公开(公告)日：2016-01-28

申请号：US14775381

申请日：2014-03-11

Applicant: ROCHE DIAGNOSTICS HEMATOLOGY, INC.

Inventor： Michael Zahniser ,  Kam Lin Wong ,  David Zahniser ,  Robert Hohlfeld

IPC: G06K9/00 ,  G02B21/36 ,  G06K9/48 ,  G06K9/62 ,  G01N15/14 ,  G06T7/00

CPC classification number: G06K9/00147 ,  G01N15/1475 ,  G01N2015/008 ,  G02B21/367 ,  G06K9/00134 ,  G06K9/0014 ,  G06K9/481 ,  G06K9/6278 ,  G06T7/0012 ,  G06T7/11 ,  G06T7/136 ,  G06T2207/10016 ,  G06T2207/10024 ,  G06T2207/10056 ,  G06T2207/20016 ,  G06T2207/30024 ,  G06T2207/30242

Abstract: This document describes methods, systems and computer program products directed to imaging blood cells. The subject matter described in this document can be embodied in a method of classifying white blood cells (WBCs) in a biological sample on a substrate. The method includes acquiring, by an image acquisition device, a plurality of images of a first location on the substrate, and classifying, by a processor, objects in the plurality of images into WBC classification groups. The method also includes identifying, by a processor, objects from at least some classification groups, as unclassified objects, and displaying, on a user interface, the unclassified objects and at least some of the classified objects.

Abstract translation: 本文档描述了针对血细胞成像的方法，系统和计算机程序产品。 本文中描述的主题可以体现在对基底上的生物样品中的白细胞（WBC）进行分类的方法中。 该方法包括由图像获取装置获取基板上的第一位置的多个图像，并且由处理器将多个图像中的对象分类为WBC分类组。 该方法还包括由处理器识别来自至少一些分类组的对象，作为未分类对象，以及在用户界面上显示未分类对象和至少一些分类对象。

公开(公告)号：US09224031B2

公开(公告)日：2015-12-29

申请号：US13699042

申请日：2011-05-18

Applicant: Martin Glensbjerg ,  Johan Christer Holm ,  Frans Ejner Ravn ,  Søren Kjaerulff

Inventor： Martin Glensbjerg ,  Johan Christer Holm ,  Frans Ejner Ravn ,  Søren Kjaerulff

IPC: G06K9/00 ,  G02B21/12 ,  G01N15/14 ,  G02B21/10 ,  G01N21/64

CPC classification number: G06K9/00147 ,  G01N15/1475 ,  G01N21/6458 ,  G01N2015/1486 ,  G01N2015/1497 ,  G02B21/10 ,  G02B21/125 ,  G06T2207/10056 ,  G06T2207/30024

Abstract: The invention relates to image analysis of dark field images obtained at low magnification below 10:1. Image analysis of dark field images obtained at low magnification can be combined with analyzes of images obtained in respect of the same section of a sample and same magnification but with other techniques such as fluorescent microscopy. The system and method can be used e.g. for particle counting, particle size measurement, particle size distribution, morphology measurement, where the particles can be cells and/or cell parts. The invention also relates to a compact dark field light source unit, a system or apparatus including a microscope which by itself is compact and comprises the mentioned dark field light source unit.

Abstract translation: 本发明涉及以低于10:1的低倍率获得的暗场图像的图像分析。 在低倍率下获得的暗场图像的图像分析可以与对样品相同部分获得的图像和相同放大倍率的分析进行组合，但与其他技术如荧光显微镜组合。 该系统和方法可以例如使用。 用于颗粒计数，粒度测量，粒度分布，形态学测量，其中颗粒可以是细胞和/或细胞部分。 本发明还涉及一种紧凑的暗场光源单元，包括本身紧凑的包括所述暗场光源单元的显微镜的系统或设备。

公开(公告)号：US09222879B2

公开(公告)日：2015-12-29

申请号：US13818188

申请日：2011-09-09

Applicant: Matthias Hartrumpf

Inventor： Matthias Hartrumpf

IPC: G01N21/21 ,  G01N15/14

CPC classification number: G01N21/21 ,  G01N15/1434 ,  G01N15/1463 ,  G01N15/1475 ,  G01N21/84 ,  G01N2015/144 ,  G01N2021/845 ,  G01N2201/0438 ,  G01N2201/06113

Abstract: The present invention relates to a device for optical characterization of a sample and/or of the material(s) of the same having an illumination unit that can be orientated to illuminate with incident light a sample spatial portion into which the sample can be introduced, a detection unit which is orientated or can be orientated to image the sample introduced into the sample spatial portion by receiving light reflected by the sample, and which is configured to detect at least two different, preferably orthogonal, polarization components in the reflected light, and an evaluation unit with which, in the imaging data recorded by the detection unit, those imaged surface elements (reflection elements) of the sample can be identified, and with which the detected different polarization components for these reflection elements can be evaluated for optical characterization.

Abstract translation: 本发明涉及一种用于光学表征样品和/或其材料的装置，该装置具有照明单元，该照明单元可以被定向为用入射光照射样品可以引入其中的样品空间部分， 一个检测单元，其被定向或可以被定向以通过接收由样本反射的光来对引入到样本空间部分中的样品成像，并且被配置为检测反射光中的至少两个不同的，优选正交的偏振分量;以及 评估单元，在由检测单元记录的成像数据中，可以识别样品的那些成像表面元件（反射元件），并且可以通过该评估单元评估用于这些反射元件的检测到的不同偏振分量用于光学表征。

公开(公告)号：US20150356731A1

公开(公告)日：2015-12-10

申请号：US14826825

申请日：2015-08-14

Applicant: FUJI XEROX CO., LTD.

Inventor： Ryota OZAKI ,  Noriji KATO ,  Hideto ODA ,  Yukio KUMAZAWA

IPC: G06T7/00 ,  G06K9/00

CPC classification number: G06T7/0012 ,  G01N15/1475 ,  G02B21/367 ,  G06K9/0014 ,  G06K9/00523 ,  G06K9/4647 ,  G06T2207/10024 ,  G06T2207/10056 ,  G06T2207/20076 ,  G06T2207/30024 ,  G06T2210/41

Abstract: A device comprises: a nucleus-candidate-region extracting section that extracts, from a captured image obtained by image-capturing a sample piece including a target cell having a nucleus, a nucleus candidate region corresponding to the nucleus; a basic-probability-information acquiring section that acquires, for each of a plurality of determination subject regions determined on the basis of the nucleus candidate region extracted by the nucleus-candidate-region extracting section, basic probability information indicating probability that an image in the determination subject region is an image of the target cell, on the basis of a feature amount of the image of the determination subject region; and a probability-information calculating section that calculates probability information indicating probability that an image in a display subject region corresponding to the nucleus candidate region is the image of the target cell, on the basis of the basic probability information acquired for each of the plurality of determination subject regions.

Abstract translation: 一种装置包括：核候选区域提取部，其从通过对包含具有核的目标细胞进行图像捕获的样本片获得的拍摄图像提取与所述细胞核相对应的核候选区域; 基本概率信息获取部，其针对由所述细胞核候选区域提取部提取的所述核候选区域确定的多个判定对象区域中的每一个取得基本概率信息，所述基本概率信息表示 基于确定对象区域的图像的特征量，确定对象区域是目标小区的图像; 以及概率信息计算部，其基于针对所述多个核心候补区域中的每一个获得的基本概率信息，计算表示与所述核候选区域对应的显示对象区域中的图像为目标小区的图像的概率的概率信息 确定科目区域。

公开(公告)号：US09074980B2

公开(公告)日：2015-07-07

申请号：US13278716

申请日：2011-10-21

Applicant: Tae-Hyun Yoon ,  Song-Hee Lee ,  Dong-Wook Kwon ,  Jong-Hoon Park ,  Hyun-Ju Yoo ,  Hyun-Woo Nho

Inventor： Tae-Hyun Yoon ,  Song-Hee Lee ,  Dong-Wook Kwon ,  Jong-Hoon Park ,  Hyun-Ju Yoo ,  Hyun-Woo Nho

IPC: C12Q1/18 ,  G01N21/64 ,  G01N21/29 ,  G01N15/14 ,  G01N15/04 ,  G01N33/50 ,  B82Y5/00 ,  B82Y35/00 ,  G01N15/00 ,  G01N15/10

CPC classification number: G01N15/1475 ,  G01N15/04 ,  G01N15/147 ,  G01N15/1484 ,  G01N21/6458 ,  G01N21/648 ,  G01N33/5014 ,  G01N2015/0038 ,  G01N2015/0092 ,  G01N2015/1075 ,  G01N2015/1486 ,  G01N2015/1497 ,  G01N2510/00

Abstract: The present invention relates to a method for the toxicity assessment of nano-materials, and more specifically, it is relates to an objective, reproducible and accurate assessment method for the unbiased toxicity testings of nano-materials, which minimize artifacts of the conventional methods for the toxicity assessment of the nano-materials by considering the dose characteristics of the nano-material itself using Selective multi-Plane Illumination Microcopy (SPIM); and the response characteristics of the nano-material using the improved or novel cellular responses assessment methods for nano-materials (e.g., modified MTT assay using image cytometric analysis, normal-inverted exposure apparatus, and modified flow cytometry), and a system and an apparatus thereof.

Abstract translation: 本发明涉及一种纳米材料的毒性评估方法，更具体地说，本发明涉及一种用于纳米材料的无偏差毒性试验的客观，可重现和准确的评估方法，其使常规方法的伪影最小化 通过使用选择性多平面照明显微镜（SPIM）考虑纳米材料本身的剂量特征，对纳米材料的毒性评估; 以及纳米材料的改进或新型细胞应答评估方法（例如，使用图像细胞计数分析，正常反转曝光装置和修饰的流式细胞术的修饰MTT测定）的纳米材料的响应特性，以及系统和 装置。