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    Probe based nucleic acid detection
    63.
    发明授权
    Probe based nucleic acid detection 有权
    基于探针的核酸检测

    公开(公告)号：US08809238B2

    公开(公告)日：2014-08-19

    申请号：US13467933

    申请日：2012-05-09

    Applicant: Kenneth J. Livak ,  Stacey N. Meyers ,  Jun Wang ,  Xiaohui Wang

    Inventor： Kenneth J. Livak ,  Stacey N. Meyers ,  Jun Wang ,  Xiaohui Wang

    IPC: G01N21/64 ,  C40B30/04 ,  C12Q1/68

    CPC classification number: C12Q1/6818 ,  C12Q2525/151

    Abstract: The invention provides a method for detecting a target nucleotide sequence by tagging the nucleotide sequence with a nucleotide tag, providing a probe oligonucleotide with a melting temperature Tm1, comprising a regulatory sequence and a nucleotide tag recognition sequence; incorporating the probe oligonucleotide into the tagged polynucleotide in a polynucleotide amplification reaction, providing a regulatory oligonucleotide with a melting temperature Tm2, comprising a sequence segment that is at least partially complementary to the regulatory sequence, amplifying the tagged target nucleic acid sequence in a PCR amplification reaction using the probe oligonucleotide as a primer, and detecting the amplification product; wherein Tm1 and Tm2 are higher than the annealing temperature associated with the polynucleotide amplification reaction.

    Abstract translation: 本发明提供了通过用核苷酸标签标记核苷酸序列来检测靶核苷酸序列的方法，提供了具有调节序列和核苷酸标签识别序列的融解温度Tm1的探针寡核苷酸; 在多核苷酸扩增反应中将探针寡核苷酸掺入标记的多核苷酸中，提供具有解链温度Tm2的调节性寡核苷酸，其包含与调节序列至少部分互补的序列片段，在PCR扩增中扩增标记的靶核酸序列 使用探针寡核苷酸作为引物的反应，检测扩增产物; 其中Tm1和Tm2高于与多核苷酸扩增反应相关的退火温度。

    Microfluidic particle-analysis systems
    66.
    发明授权
    Microfluidic particle-analysis systems 有权
    微流控粒子分析系统

    公开(公告)号：US08658418B2

    公开(公告)日：2014-02-25

    申请号：US12501982

    申请日：2009-07-13

    Applicant: Antoine Daridon

    Inventor： Antoine Daridon

    IPC: C12M1/34 ,  C12M3/00

    CPC classification number: C12M1/34 ,  B01L3/502738 ,  B01L3/502746 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2200/16 ,  B01L2300/06 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0681 ,  B01L2300/0861 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/088 ,  B01L2300/0887 ,  B01L2300/0893 ,  B01L2300/123 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/0481 ,  B01L2400/0622 ,  B01L2400/0655 ,  C12M21/06 ,  C12M23/16 ,  G01N15/1484 ,  G01N33/4833 ,  G01N2015/008 ,  G01N2015/0288 ,  G01N2015/149 ,  G01N2015/1493 ,  G02B21/32

    Abstract: The invention provides systems, including apparatus, methods, and kits, for the microfluidic manipulation and/or detection of particles, such as cells and/or beads. The invention provides systems, including apparatus, methods, and kits, for the microfluidic manipulation and/or analysis of particles, such as cells, viruses, organelles, beads, and/or vesicles. The invention also provides microfluidic mechanisms for carrying out these manipulations and analysis. These mechanisms may enable controlled input, movement/positioning, retention/localization, treatment, measurement, release, and/or output of particles. Furthermore, these mechanisms may be combined in any suitable order and/or employed for any suitable number of times within a system. Accordingly, these combinations may allow particles to be sorted, cultured, mixed, treated, and/or assayed, among others, as single particles, mixed groups of particles, arrays of particles, heterogeneous particle sets, and/or homogeneous particle sets, among others, in series and/or in parallel. In addition, these combinations may enable microfluidic systems to be reused. Furthermore, these combinations may allow the response of particles to treatment to be measured on a shorter time scale than was previously possible. Therefore, systems of the invention may allow a broad range of cell and particle assays, such as drug screens, cell characterizations, research studies, and/or clinical analysis, among others, to be scaled down to microfluidic size. Such scaled-down assays may use less sample and reagent, may be less labor intensive, and/or may be more informative than comparable macrofluidic assays.

    Abstract translation: 本发明提供了用于微流体操纵和/或检测诸如细胞和/或珠粒的装置，方法和试剂盒的系统。 本发明提供了用于微粒操作和/或分析颗粒例如细胞，病毒，细胞器，珠粒和/或囊泡的装置，方法和试剂盒的系统。 本发明还提供了用于进行这些操作和分析的微流体机构。 这些机制可以实现颗粒的控制输入，运动/定位，保留/定位，处理，测量，释放和/或输出。 此外，这些机制可以以任何合适的顺序组合和/或在系统内任何适当次数使用。 因此，这些组合可以允许将粒子分类，培养，混合，处理和/或测定为单粒子，粒子的混合组，粒子阵列，非均匀粒子组和/或均匀粒子组，其中 其他，串联和/或并行。 此外，这些组合可以使微流体系统能够重复使用。 此外，这些组合可以允许在比以前可能的更短的时间尺度上测量颗粒对治疗的反应。 因此，本发明的系统可以允许广泛范围的细胞和颗粒测定，例如药物筛选，细胞特征化，研究研究和/或临床分析等等，以缩小至微流体大小。 这种按比例缩小的测定可能使用较少的样品和试剂，可能较少的劳动密集型和/或可能比可比较的大流控测定更具信息性。

    COPY NUMBER VARIATION DETERMINATION, METHODS AND SYSTEMS
    69.
    发明申请
    COPY NUMBER VARIATION DETERMINATION, METHODS AND SYSTEMS 审中-公开

    公开(公告)号：US20130260381A1

    公开(公告)日：2013-10-03

    申请号：US13902667

    申请日：2013-05-24

    Applicant: Fluidigm Corporation

    Inventor： Ramesh Ramakrishnan

    IPC: C12Q1/68

    CPC classification number: C12Q1/6869 ,  C12Q1/6851 ,  C12Q2545/101 ,  C12Q2527/143

    Abstract: The present invention methods and systems for determining copy number variation of a target polynucleotide in a genome of a subject including amplification based techniques. Methods can include pre-amplification of the sample followed by distribution of sample and a plurality of reaction volumes, quantitative detection of a target polynucleotide and a reference polynucleotide, and analysis so as to determine the relative copy number of the target polynucleotide sequence in the genome of the subject.

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