公开(公告)号：US09709559B2

公开(公告)日：2017-07-18

申请号：US10032657

申请日：2001-12-28

Applicant: Sukanta Banerjee ,  Michael Seul

Inventor： Sukanta Banerjee ,  Michael Seul

IPC: G01N33/543 ,  G01N33/58 ,  B01J19/00 ,  B82Y15/00

CPC classification number: G01N33/5434 ,  B01J19/0046 ,  B01J2219/005 ,  B01J2219/00545 ,  B01J2219/00576 ,  B82Y15/00 ,  C12Q1/6837 ,  C12Q1/6876 ,  G01N27/745 ,  G01N33/54326 ,  G01N33/54333 ,  G01N33/54346 ,  G01N33/587 ,  G01N33/588

Abstract: The present invention provides a method for the generation of novel libraries of encoded magnetic particles from sub-libraries of by the generation of novel sub-libraries of magnetic nanoparticles and encoded particles. The sub-libraries are functionalized on demand are useful in the formation of arrays. The present invention is especially useful for performing multiplexed (parallel) assays for qualitative and/or quantitative analysis of binding interactions of a number of analyte molecules in a sample.

公开(公告)号：US20170184579A1

公开(公告)日：2017-06-29

申请号：US15456049

申请日：2017-03-10

Applicant: Silicon BioDevices, Inc.

Inventor： Octavian FLORESCU ,  Daniel WONG ,  Tracie MARTIN ,  Duane YAMASAKI ,  Remy CROMER

IPC: G01N33/543 ,  C12Q1/68 ,  G06K19/06 ,  B01L3/00

CPC classification number: G01N33/54326 ,  B01L3/502761 ,  B01L3/561 ,  B01L2200/0605 ,  B01L2200/0668 ,  B01L2200/148 ,  B01L2200/16 ,  B01L2300/023 ,  B01L2300/027 ,  B01L2300/0636 ,  B01L2300/0838 ,  B01L2300/0874 ,  B01L2300/12 ,  B01L2400/0406 ,  B01L2400/043 ,  C12Q1/686 ,  G01N21/255 ,  G01N21/82 ,  G01N27/745 ,  G01N2201/061 ,  G01N2201/068 ,  G06F21/602 ,  G06K19/06028 ,  G06K19/06037 ,  G16H50/20 ,  Y02A90/26

Abstract: An assay system and method for use in the field of chemical testing is disclosed. The assay system can be used for filtering whole blood for testing on an integrated circuit containing digital control functionality.

公开(公告)号：US20170184505A1

公开(公告)日：2017-06-29

申请号：US15055982

申请日：2016-02-29

Applicant: Jin Z. Zhang ,  Adam Schwartzberg ,  Tammy Y. Olson

Inventor： Jin Z. Zhang ,  Adam Schwartzberg ,  Tammy Y. Olson

IPC: G01N21/65 ,  B22F9/24 ,  B22F1/00 ,  H01L29/06 ,  G01N33/543

CPC classification number: G01N21/658 ,  B22F1/0025 ,  B22F9/24 ,  B22F2001/0029 ,  B22F2009/245 ,  B22F2301/255 ,  B22F2304/05 ,  B22F2998/00 ,  B22F2998/10 ,  B82Y10/00 ,  B82Y15/00 ,  B82Y25/00 ,  B82Y30/00 ,  B82Y35/00 ,  G01N21/554 ,  G01N33/5432 ,  G01N33/54326 ,  G01N33/54346 ,  G01N2201/06113 ,  H01F1/0054 ,  H01L29/0665 ,  H01L29/0669 ,  H01L29/0673 ,  H01L29/127 ,  Y10S977/762 ,  Y10S977/81 ,  Y10S977/896 ,  Y10S977/958 ,  Y10T428/12431 ,  Y10T428/2975 ,  Y10T428/2982

Abstract: The invention is drawn to novel nanostructures comprising hollow nanospheres and nanotubes for use as chemical sensors, conduits for fluids, and electronic conductors. The nanostructures can be used in microfluidic devices, for transporting fluids between devices and structures in analytical devices, for conducting electrical currents between devices and structure in analytical devices, and for conducting electrical currents between biological molecules and electronic devices, such as bio-microchips.

公开(公告)号：US20170182495A1

公开(公告)日：2017-06-29

申请号：US15457459

申请日：2017-03-13

Applicant: The Research Foundation for The State University of New York

Inventor： Helmut Strey ,  Robert Kimmerling ,  Tomasz Bakowski

IPC: B01L3/00 ,  G01N33/543 ,  C12N15/10 ,  C12Q1/68

CPC classification number: B01L3/502784 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502761 ,  B01L3/502776 ,  B01L7/52 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2300/0864 ,  B01L2400/043 ,  B01L2400/0487 ,  C12N15/1096 ,  C12Q1/686 ,  G01N33/54313 ,  G01N33/54326 ,  G01N33/54366 ,  C12Q2563/159 ,  C12Q2565/629

Abstract: The compositions and methods described herein are designed to introduce functionalized microparticles into droplets that can be manipulated in microfluidic devices by fields, including electric (dielectrophoretic) or magnetic fields, and extracted by splitting a droplet to separate the portion of the droplet that contains the majority of the microparticles from the part that is largely devoid of the microparticles. Within the device, channels are variously configured at Y- or T junctions that facilitate continuous, serial isolation and dilution of analytes in solution. The devices can be limited in the sense that they can be designed to output purified analytes that are then further analyzed in separate machines or they can include additional channels through which purified analytes can be further processed and analyzed.

公开(公告)号：US20170159113A1

公开(公告)日：2017-06-08

申请号：US15241844

申请日：2016-08-19

Applicant: T2 Biosystems, Inc.

Inventor： Lori Anne NEELY ,  Brian M. MOZELESKI ,  Mark John AUDEH ,  Jordan R. RAPHEL ,  Thomas Jay LOWERY, Jr.

IPC: C12Q1/68 ,  G01N33/543

CPC classification number: C12Q1/6834 ,  G01N33/53 ,  G01N33/5304 ,  G01N33/5308 ,  G01N33/543 ,  G01N33/54326 ,  C12Q2537/125 ,  C12Q2537/149 ,  C12Q2563/143 ,  C12Q2563/149 ,  C12Q2565/113

Abstract: The present invention relates to methods and compositions for detecting analytes, including proteins, polysaccharides, viruses, nucleic acids and cells. The methods and compositions utilize a reporter probe, suitably a multivalent reporter probe, to detect the presence of the analytes. In embodiments, the methods and compositions can be used for non-enzymatic detection of nucleic acids.

公开(公告)号：US09671395B2

公开(公告)日：2017-06-06

申请号：US13091548

申请日：2011-04-21

Applicant: Sergey A. Dryga ,  Lisa-Jo Ann Clarizia ,  Eddie W. Adams ,  Victor C. Esch

Inventor： Sergey A. Dryga ,  Lisa-Jo Ann Clarizia ,  Eddie W. Adams ,  Victor C. Esch

IPC: C12Q1/00 ,  C12Q1/68 ,  G01N33/53 ,  G01N33/554 ,  G01N33/543 ,  G01N33/569 ,  C07K1/22 ,  C07K16/12 ,  C12N13/00 ,  G01N24/08 ,  G01R33/465 ,  A61B5/055

CPC classification number: G01N33/54326 ,  A61B5/055 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2400/043 ,  C07K1/22 ,  C07K16/1267 ,  C12N13/00 ,  C12N15/1013 ,  C12Q1/6806 ,  C12Q1/689 ,  C12Q2565/633 ,  G01N24/08 ,  G01N33/54333 ,  G01N33/56911 ,  G01N2333/195 ,  G01N2446/20 ,  G01R33/465 ,  Y10T428/2982 ,  C12Q2565/629 ,  C12Q2563/143

Abstract: The invention generally relates to conducting an assay on a sample that isolates a pathogen from the sample and allows for analysis of the pathogen with minimal (i.e., at most 24 hrs of culturing) or no culturing of the pathogen. In certain embodiments, the invention provides methods for identifying a pathogen from a sample that involve obtaining a sample including a pathogen, conducting an assay that isolates the pathogen from the sample, culturing the isolated pathogen for at most about 24 hrs, and analyzing the pathogen.

公开(公告)号：US09663818B2

公开(公告)日：2017-05-30

申请号：US14408065

申请日：2013-06-14

Applicant: The University of Chicago

Inventor： Amy Flor ,  Ryan Duggan ,  Stephen Kron

IPC: G01N15/14 ,  C12Q1/68 ,  G01N33/543 ,  G01N33/569 ,  G01N21/64 ,  G01N33/68 ,  G01N15/10 ,  G01N15/00

CPC classification number: C12Q1/6804 ,  C12Q1/6811 ,  G01N15/14 ,  G01N21/6486 ,  G01N33/54326 ,  G01N33/54333 ,  G01N33/56966 ,  G01N33/56972 ,  G01N33/6854 ,  G01N2015/0065 ,  G01N2015/008 ,  G01N2015/1006 ,  G01N2015/1402 ,  G01N2458/10

Abstract: Methods and compositions for quantitative immunoassays are provided, in which ligand-conjugated probes are used to label samples and ligand-surfaced microspheres are used as quantitative reference standards. Certain embodiments provide a method of quantitative flow cytometry where ligands are oligonucleotides, and a sample comprising one or more cells is contacted with a hybridized antibody::fluorophore labeled targeting construct to label the cells, and the labeled cells are analyzed. In some embodiments, a population of quantitative labeled oligospheres labeled with the same fluorescent label as the cells is analyzed using the flow cytometer and used to create a quantitative standard curve of cytometer intensity versus molecules fluorescent label per oligosphere event. A standard curve trendline is established and used to determine the molecules of fluorescent label per cellular event for the antigen-positive cell populations. Based on molecules of fluorescent label per cell, the amount of Antibody Binding per Cell (ABC) is quantified.

公开(公告)号：US09658226B2

公开(公告)日：2017-05-23

申请号：US14673647

申请日：2015-03-30

Applicant: Hycor Biomedical, Inc.

Inventor： Mark David Van Cleve ,  Elaine Grace Taine ,  Douglas John Canfield ,  Stephanie TuVi Ortega ,  Taylor Addison Reid

IPC: G01N33/564 ,  G01N35/10 ,  G01N21/64 ,  G01N33/543 ,  G01N33/68 ,  G01N33/53 ,  G01N33/58 ,  G01N35/00 ,  G01N21/76 ,  G01N33/569 ,  G01N35/04

CPC classification number: G01N21/645 ,  G01N21/6428 ,  G01N21/76 ,  G01N33/5306 ,  G01N33/54326 ,  G01N33/5434 ,  G01N33/54393 ,  G01N33/564 ,  G01N33/569 ,  G01N33/5695 ,  G01N33/56983 ,  G01N33/582 ,  G01N33/6854 ,  G01N33/6893 ,  G01N35/0098 ,  G01N35/1011 ,  G01N2021/6484 ,  G01N2035/0453 ,  G01N2035/1062 ,  G01N2201/062 ,  G01N2201/08 ,  G01N2333/4703 ,  G01N2333/62 ,  G01N2333/78 ,  G01N2800/24 ,  Y10T436/119163

Abstract: A quantitative method for diagnosing an autoimmune disease or an infectious disease comprising performing an automated diagnostic assay, comprising: incubating a capture reagent with a streptavidin-coated medium to form a solid phase complex, wherein the capture reagent is a biotinylated autoantigen or infectious disease antigen; washing the solid phase complex to remove excess capture reagent; incubating the solid phase complex with a serum sample to form an immune complex; washing the immune complex to remove any unbound sample; incubating the immune complex with a conjugate to create an immune-conjugate complex; washing the immune-conjugate complex to remove any unbound conjugate; introducing a substrate capable of generating a quantifiable response; and calibrating the response generated from introducing the substrate.

公开(公告)号：US20170115283A1

公开(公告)日：2017-04-27

申请号：US15402879

申请日：2017-01-10

Applicant: Promega Corporation

Inventor： Carolyn W. Hitko ,  Robin Hurst ,  Thomas Kirkland ,  Dieter Klaubert ,  Poncho Meisenheimer ,  Nidhi Nath ,  Rachel Friedman Ohana ,  Paul Otto ,  Marjeta Urh ,  Harry Tetsuo Uyeda ,  Keith Wood ,  Ji Zhu

IPC: G01N33/543

CPC classification number: G01N33/54333 ,  G01N33/54306 ,  G01N33/54326

Abstract: The present invention provides compositions and methods for capture and identification of the cellular targets of a bioactive agent. In particular, provided herein are bioactive agents tethered to capture ligand, cellular targets (optionally tagged with a reporter), capture proteins (optionally present as capture fusions), surfaces (e.g., displaying, capture ligands, capture proteins, or capture fusions), and methods of capturing and identifying the cellular targets of a bioactive agent therewith.

公开(公告)号：US20170108497A1

公开(公告)日：2017-04-20

申请号：US15392895

申请日：2016-12-28

Applicant: Silicon BioDevices, Inc.

Inventor： Octavian FLORESCU

IPC: G01N33/543 ,  G01N15/14 ,  G01V8/20 ,  G01N21/78

CPC classification number: G01N33/54366 ,  G01N15/1436 ,  G01N21/17 ,  G01N21/253 ,  G01N21/274 ,  G01N21/78 ,  G01N33/54326 ,  G01N2201/062 ,  G01V8/20

Abstract: A biosensor system and method of its use for detecting particles on the surface of an integrated circuit is disclosed. The system can include a light source and a plurality of optical sensors formed on an integrated circuit. The particles can be positioned the surface of the integrated circuit whereby the particles can cast a shadow or shadows that reduces the amount of light transmitted from the light source to the optical sensors. The surface of the integrated circuit can include one or more optical sensing areas whereby the presence of one or more particles may significantly or measurably reduce the amount of light incident on one or more optical sensor.