-
公开(公告)号：US20250091048A1
公开(公告)日：2025-03-20
申请号：US18888785
申请日：2024-09-18
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Benjamin J. Hindson , Serge Saxonov , Phillip Belgrader , Kevin D. Ness , Michael Y. Lucero , Billy W. Colston, JR. , Shawn Paul Hodges , Nicholas J. Heredia , Jeffrey Clark Mellen , Camille Bodley Troup , Paul Wyatt
IPC: B01L3/00 , B01F23/41 , B01F25/00 , B01F25/23 , B01F33/3011 , B01F33/302 , B01F33/81 , B01L9/00 , C12Q1/686 , G01N35/08
Abstract: The present disclosure provides methods and compositions for detecting polynucleotides in a sample and for quantifying polynucleotide load in a sample. The polynucleotides can be associated with a disease, disorder, or condition. In some applications, methylated DNA is quantified, e.g., in order to determine the load of polynucleotides in a sample. The present disclosure also provides methods and compositions for determining the load of fetal polynucleotides in a biological sample, e.g., the load of fetal polynucleotides (e.g., DNA, RNA) in maternal plasma. The present disclosure provides methods and compositions for detecting cellular processes such as cellular viability, growth rates, and infection rates. This disclosure also provides compositions and methods for detecting differences in copy number of a target polynucleotide. In some embodiments, the methods and compositions provided herein are useful for diagnosis of fetal genetic abnormalities, when the starting sample is maternal tissue (e.g., blood, plasma). The methods and materials described apply techniques for allowing detection of small, but statistically significant, differences in polynucleotide copy number.
-
公开(公告)号：US12168231B2
公开(公告)日：2024-12-17
申请号：US17486804
申请日：2021-09-27
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Billy W. Colston, Jr. , Benjamin J. Hindson , Kevin D. Ness , Donald A. Masquelier
IPC: B01L3/00 , B01F23/41 , B01F33/3011 , B01F35/10 , B01L3/02 , B01L7/00 , B29C45/00 , C12Q1/686 , G01N21/3563 , G01N21/49 , G01N21/64 , B01F101/23 , B29L31/00
Abstract: Method of analysis. In the method, a microfluidic device defining a flow path extending from an inlet to an outlet may be selected. A sample-containing fluid may be introduced into the flow path via the inlet. Volumes of the sample-containing fluid may be isolated from one another on the flow path. A two-dimensional monolayer of the volumes may be imaged. The two-dimensional monolayer may be formed along the flow path between the inlet and the outlet.
-
公开(公告)号：US12097495B2
公开(公告)日：2024-09-24
申请号：US17750195
申请日：2022-05-20
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Benjamin J. Hindson , Serge Saxonov , Phillip Belgrader , Kevin D. Ness , Michael Y. Lucero , Billy W. Colston, Jr. , Shawn Paul Hodges , Nicholas J. Heredia , Jeffrey Clark Mellen , Camille Bodley Troup , Paul Wyatt
IPC: B01L3/00 , B01F23/41 , B01F33/3011 , B01F33/81 , B01L9/00 , C12Q1/686 , B01F25/00 , B01F25/23 , B01F33/302 , G01N35/08
CPC classification number: B01L3/50273 , B01F23/41 , B01F33/3011 , B01F33/813 , B01L3/502 , B01L3/502784 , B01L3/52 , B01L9/527 , C12Q1/686 , B01F25/14 , B01F25/23 , B01F33/3021 , B01L3/502761 , B01L2200/025 , B01L2200/0636 , B01L2200/0647 , B01L2200/0673 , B01L2200/14 , B01L2200/16 , B01L2300/0609 , B01L2300/0681 , B01L2300/0816 , B01L2300/0829 , B01L2300/0861 , B01L2400/049 , G01N35/08
Abstract: The present disclosure provides methods and compositions for detecting polynucleotides in a sample and for quantifying polynucleotide load in a sample. The polynucleotides can be associated with a disease, disorder, or condition. In some applications, methylated DNA is quantified, e.g., in order to determine the load of polynucleotides in a sample. The present disclosure also provides methods and compositions for determining the load of fetal polynucleotides in a biological sample, e.g., the load of fetal polynucleotides (e.g., DNA, RNA) in maternal plasma. The present disclosure provides methods and compositions for detecting cellular processes such as cellular viability, growth rates, and infection rates. This disclosure also provides compositions and methods for detecting differences in copy number of a target polynucleotide. In some embodiments, the methods and compositions provided herein are useful for diagnosis of fetal genetic abnormalities, when the starting sample is maternal tissue (e.g., blood, plasma). The methods and materials described apply techniques for allowing detection of small, but statistically significant, differences in polynucleotide copy number.
-
公开(公告)号：US10676778B2
公开(公告)日：2020-06-09
申请号：US15818601
申请日：2017-11-20
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Adam Lowe , Geoff McDermott , Christopher M. Hindson , Erin R. Chia , Amy L. Hiddessen , Benjamin J. Hindson , Chunxiao Han , Yaqi Wang
IPC: C12Q1/68 , C12Q1/6804 , B01L7/00 , C12Q1/6844 , C12Q1/6851 , B01L3/00
Abstract: Methods, devices, systems and compositions for detecting nucleic acids in polymerase chain reaction assays, such as droplet digital polymerase chain reaction (ddPCR) assays, using intercalating dyes. A dual surfactant system with at least one fluorosurfactant and at least one non-ionic non-fluorosurfactant may be employed for droplet generation and nucleic acid detection.
-
公开(公告)号：US20180147573A1
公开(公告)日：2018-05-31
申请号：US15707908
申请日：2017-09-18
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Amy L. Hiddessen , Donald A. Masquelier , Kevin D. Ness , Benjamin J. Hindson , Anthony J. Makarewicz, Jr. , Erin R. Chia , Billy W. Colston, Jr. , Serge Saxonov , Svilen S. Tzonev , Michael Y. Lucero , Ryan T. Koehler
IPC: B01L3/00 , B01L9/00 , B01F13/00 , C12Q1/686 , B01F13/10 , B01F3/08 , B01F5/02 , B01F5/00 , G01N35/08
CPC classification number: B01L3/50273 , B01F3/0807 , B01F5/0085 , B01F5/0256 , B01F13/0062 , B01F13/0071 , B01F13/1022 , B01L3/502 , B01L3/502761 , B01L3/502784 , B01L3/52 , B01L9/527 , B01L2200/025 , B01L2200/0636 , B01L2200/0647 , B01L2200/0673 , B01L2200/14 , B01L2200/16 , B01L2300/0609 , B01L2300/0681 , B01L2300/0816 , B01L2300/0829 , B01L2300/0861 , B01L2400/049 , C12Q1/686 , G01N35/08
Abstract: Devices and methods for generating droplets. An exemplary device comprises a substantially planar base portion including a bottom surface having a plurality of microfluidic channels formed therein as recessed regions of the bottom surface. The device also comprises a plurality of protrusions projecting from a top surface of the base portion and each formed integrally with the base portion. The device further comprises a sample well, a carrier well, and a droplet well. Each well has an upper portion created by one of the protrusions. A cover layer is attached to the bottom surface of the base portion and seals a bottom side of each microfluidic channel.
-
Patent Agency Ranking
公开(公告)号：US09901927B2
公开(公告)日：2018-02-27
申请号：US15365894
申请日：2016-11-30
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Benjamin J. Hindson , Kevin D. Ness , Billy W. Colston, Jr. , Fred P. Milanovich , Donald A. Masquelier
IPC: B01L3/00 , B01F3/08 , B01F13/00 , B01L3/02 , B01L7/00 , G01N21/3563 , C12Q1/68 , G01N21/64 , B01F15/00 , B29C45/00 , G01N21/49 , B29L31/00
Abstract: Methods of generating droplets. In an exemplary method, a device including a sample well, a carrier well, a droplet well, and a plurality of microfluidic channels is selected. The microfluidic channels include a first channel, a second channel, and a third channel. A discrete volume of sample-containing fluid is placed into the sample well, and a discrete volume of carrier fluid is placed into the carrier well. A pressure differential is created after placing the discrete volumes, to cause fluid flow. Sample-containing fluid flows from the sample well to a droplet-generation region of the device via the first channel. Carrier fluid flows from the carrier well to the droplet-generation region via the second channel. Sample-containing droplets and carrier fluid flow from the droplet-generation region to the droplet well via the third channel.
-
公开(公告)号：US20170144161A1
公开(公告)日：2017-05-25
申请号：US15365894
申请日：2016-11-30
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Benjamin J. Hindson , Kevin D. Ness , Billy W. Colston, JR. , Fred P. Milanovich , Donald A. Masquelier , Anthony J. Makarewicz, JR.
CPC classification number: B01L3/502784 , B01F3/0807 , B01F13/0062 , B01F15/00922 , B01F2003/0834 , B01F2003/0842 , B01F2215/0037 , B01L3/0241 , B01L3/502715 , B01L7/52 , B01L7/525 , B01L2200/0673 , B01L2200/0689 , B01L2200/10 , B01L2200/12 , B01L2300/041 , B01L2300/0654 , B01L2300/0816 , B01L2300/0819 , B01L2300/0858 , B01L2300/0867 , B01L2300/1822 , B01L2400/0478 , B01L2400/0487 , B01L2400/049 , B01L2400/0622 , B29C45/0053 , B29C45/006 , B29C2045/0079 , B29L2031/752 , C12Q1/686 , G01N21/3563 , G01N21/49 , G01N21/6428 , G01N21/6486 , G01N2021/6439 , G06F19/00 , Y02A90/26
Abstract: Methods of generating droplets. In an exemplary method, a device including a sample well, a carrier well, a droplet well, and a plurality of microfluidic channels is selected. The microfluidic channels include a first channel, a second channel, and a third channel. A discrete volume of sample-containing fluid is placed into the sample well, and a discrete volume of carrier fluid is placed into the carrier well. A pressure differential is created after placing the discrete volumes, to cause fluid flow. Sample-containing fluid flows from the sample well to a droplet-generation region of the device via the first channel. Carrier fluid flows from the carrier well to the droplet-generation region via the second channel. Sample-containing droplets and carrier fluid flow from the droplet-generation region to the droplet well via the third channel.
-
公开(公告)号：US09417190B2
公开(公告)日：2016-08-16
申请号：US13945661
申请日：2013-07-18
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Benjamin J. Hindson , Billy W. Colston, Jr. , Kevin D. Ness , Donald A. Masquelier
CPC classification number: G01N21/6486 , C12Q1/6848 , G01N21/6428 , G01N21/6456 , G01N2021/6441 , G01N2201/1242 , C12Q2545/101 , C12Q2563/107 , C12Q2563/159
Abstract: System, including methods and apparatus, for performing droplet-based assays that are controlled and/or calibrated using signals detected from droplets.
Abstract translation: 用于执行使用从液滴检测的信号来控制和/或校准的基于液滴的测定的系统，包括方法和装置。
-
公开(公告)号：US20160059204A1
公开(公告)日：2016-03-03
申请号：US14938470
申请日：2015-11-11
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Benjamin J. Hindson , Billy W. Colston, JR. , Kevin D. Ness , Donald A. Masquelier
CPC classification number: B01J19/0046 , B01F13/0071 , B01F13/0079 , B01J19/0093 , B01J2219/00286 , B01J2219/00418 , B01J2219/00495 , B01J2219/0059 , B01J2219/00599 , B01J2219/00722 , B01J2219/00873 , B01J2219/00894 , B01J2219/00903 , B01J2219/00957 , B01L3/502784 , B01L7/525 , B01L2200/0673 , B01L2200/16 , B01L2300/0867 , B01L2300/0883 , B01L2300/18 , B01L2400/0487 , B01L2400/0677 , C12Q1/6844 , C40B50/08 , C40B60/14 , G01N1/30 , G01N1/44 , G01N21/75 , G01N33/50 , G01N35/08 , C12Q2547/107
Abstract: System, including methods, apparatus, compositions, and kits, for the mixing of small volumes of fluid by coalescence of multiple emulsions.
Abstract translation: 系统，包括方法，装置，组合物和试剂盒，用于通过多个乳液的聚结来混合小体积的流体。
-
公开(公告)号：US12090480B2
公开(公告)日：2024-09-17
申请号：US18362530
申请日：2023-07-31
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Donald A. Masquelier , Kevin D. Ness , Benjamin J. Hindson , Billy W. Colston, Jr.
IPC: B01L3/00 , B01F23/41 , B01F33/3011 , B01F33/81 , B01L9/00 , C12Q1/686 , B01F25/00 , B01F25/23 , B01F33/302 , G01N35/08
CPC classification number: B01L3/50273 , B01F23/41 , B01F33/3011 , B01F33/813 , B01L3/502 , B01L3/502784 , B01L3/52 , B01L9/527 , C12Q1/686 , B01F25/14 , B01F25/23 , B01F33/3021 , B01L3/502761 , B01L2200/025 , B01L2200/0636 , B01L2200/0647 , B01L2200/0673 , B01L2200/14 , B01L2200/16 , B01L2300/0609 , B01L2300/0681 , B01L2300/0816 , B01L2300/0829 , B01L2300/0861 , B01L2400/049 , G01N35/08
Abstract: Methods of partition-based analysis. In an exemplary method, a device having a port fluidically connected to a chamber may be selected. A sample-containing fluid may be placed into the port. The sample-containing fluid may be moved from the port to the chamber. Partitions of the sample-containing fluid may be formed. A monolayer of the partitions in the chamber may be created. At least a portion of the monolayer may be imaged.
-
-
-
-
-
-
-
-
-
Search Results
50
records
(took 0.065 seconds)
