利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

598 条记录 (耗时 0.035 秒)

    Liquid bridge and system
    24.
    发明授权
    Liquid bridge and system 有权
    液桥和系统

    公开(公告)号：US08298833B2

    公开(公告)日：2012-10-30

    申请号：US12092261

    申请日：2007-02-07

    申请人： Mark Davies ,  Tara Dalton ,  Kieran Curran

    发明人： Mark Davies ,  Tara Dalton ,  Kieran Curran

    IPC分类号： G01N1/10

    CPC分类号： B01L3/502784 ,  B01F13/0071 ,  B01F15/0201 ,  B01F15/0232 ,  B01F2215/0037 ,  B01L3/5025 ,  B01L3/502715 ,  B01L3/502746 ,  B01L7/525 ,  B01L2200/0673 ,  B01L2300/0829 ,  B01L2300/0838 ,  B01L2400/0478 ,  B01L2400/0487 ,  B01L2400/0633 ,  B01L2400/082 ,  C12Q1/686 ,  F17D1/08 ,  Y10T137/87676 ,  Y10T436/117497 ,  Y10T436/118339 ,  Y10T436/2575

    摘要： A bridge (30) comprises a first inlet port (31) at the end of a capillary, a narrower second inlet port (32) which is an end of a capillary, an outlet port (33) which is an end of a capillary, and a chamber (34) for silicone oil. The oil is density-matched with the reactor droplets such that a neutrally buoyant environment is created within the chamber (34). The oil within the chamber is continuously replenished by the oil separating the reactor droplets. This causes the droplets to assume a stable capillary-suspended spherical form upon entering the chamber (34). The spherical shape grows until large enough to span the gap between the ports, forming an axisymmetric liquid bridge. The introduction of a second droplet from the second inlet port (32) causes the formation of an unstable funicular bridge that quickly ruptures from the, finer, second inlet port (32), and the droplets combine at the liquid bridge (30). In another embodiment, a droplet (55) segments into smaller droplets which bridge the gap between the inlet and outlet ports.

    摘要翻译： 桥（30）包括在毛细管端部的第一入口（31），作为毛细管端部的较窄的第二入口（32），作为毛细管的端部的出口（33） 和用于硅油的室（34）。 油与反应器液滴密度匹配，使得在室（34）内产生中性浮力的环境。 室内的油通过分离反应器液滴的油不断补充。 这使得液滴在进入室（34）时呈现稳定的毛细管悬浮球形。 球形增长直到足够大以跨越端口之间的间隙，形成轴对称液桥。 从第二入口端口（32）引入第二液滴导致形成不稳定的索道桥，其从较细的第二入口（32）快速破裂，并且液滴在液体桥（30）处结合。 在另一个实施例中，液滴（55）分段成较小的液滴，桥接入口和出口之间的间隙。

    Flow cytometer sorter
    25.
    发明授权
    Flow cytometer sorter 有权
    流式细胞仪分选仪

    公开(公告)号：US08290625B2

    公开(公告)日：2012-10-16

    申请号：US12098340

    申请日：2008-04-04

    申请人： Jeffrey W. Degeal ,  Charles S. Bay ,  Edward A. Stanton ,  Paul Barclay Purcell ,  George C. Malachowski

    发明人： Jeffrey W. Degeal ,  Charles S. Bay ,  Edward A. Stanton ,  Paul Barclay Purcell ,  George C. Malachowski

    IPC分类号： G05B21/00 ,  G05B13/04 ,  G01N35/08

    CPC分类号： G06F17/00 ,  B07C5/342 ,  B07C5/361 ,  C12Q1/04 ,  G01N15/1459 ,  G01N2015/1006 ,  G01N2015/1488 ,  G01N2015/149 ,  Y10T436/11 ,  Y10T436/117497 ,  Y10T436/118339

    摘要： Disclosed are computer-implemented methods of sorting particles from a particle stream in a flow cytometer. The methods include: calculating sort decision making parameters using the raw event data values from a flow cytometer and a sort logic; performing sort logic computations using the sort logic definition and the sort decision making parameters to generate sort decisions; converting the sort decisions into sort commands; and sending the one or more sort commands to the flow cytometer. Sort logic computations may include algorithmically using conditional branching logic, and may include sort logic equations having mathematical functions characterizing one or more regions of interest in multidimensional data space. Such mathematical functions may be determined based on one or more parameters provided by a user. Also disclosed are corresponding systems having a flow cytometer and a computer.

    摘要翻译： 公开了在流式细胞仪中从粒子流中分选颗粒的计算机实现的方法。 方法包括：使用来自流式细胞仪和分类逻辑的原始事件数据值来计算排序决策参数; 使用排序逻辑定义和排序决策参数来执行排序逻辑计算以生成排序决定; 将排序决定转换为排序命令; 并将一个或多个排序命令发送到流式细胞仪。 排序逻辑计算可以包括在算法上使用条件分支逻辑，并且可以包括具有表征多维数据空间中的一个或多个感兴趣区域的数学函数的排序逻辑方程。 可以基于用户提供的一个或多个参数来确定这样的数学函数。 还公开了具有流式细胞仪和计算机的对应系统。

    Microfluidic system and method for sorting cell clusters and for the continuous encapsulation thereof following sorting thereof
    26.
    发明授权
    Microfluidic system and method for sorting cell clusters and for the continuous encapsulation thereof following sorting thereof 有权
    用于分选细胞簇的微流体系统和方法以及在分选细胞簇后的连续包封

    公开(公告)号：US08263023B2

    公开(公告)日：2012-09-11

    申请号：US12437901

    申请日：2009-05-08

    申请人： Sophie Le Vot ,  Jean Berthier ,  Florence Rivera

    发明人： Sophie Le Vot ,  Jean Berthier ,  Florence Rivera

    IPC分类号： G01N15/06 ,  G01N33/00 ,  G01N33/48

    CPC分类号： B01F13/0062 ,  B01F3/0807 ,  B01F2003/0842 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2300/0654 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/0677 ,  B01L2400/0688 ,  B01L2400/086 ,  Y10T436/11 ,  Y10T436/117497 ,  Y10T436/118339 ,  Y10T436/25 ,  Y10T436/2525

    摘要： A microfluidic system and method for sorting cell clusters, and for the continuous and automated encapsulation of the clusters, once sorted, in capsules of sizes suitable for those of these sorted clusters is provided. The microfluidic system comprises a substrate in which a microchannel array comprising a cell sorting unit is etched and around which a protective cover is bonded, and the sorting unit comprises deflection means capable of separating, during the flow thereof, relatively noncohesive cell clusters, each of size ranging from 20 μm to 500 μm and of 20 to 10 000 cells approximately, such as islets of Langerhans, at least two sorting microchannels arranged in parallel at the outlet of said unit being respectively designed so as to transport as many categories of sorted clusters continuously to a unit for encapsulation of the latter, also formed in said array.

    摘要翻译： 提供了一种用于分选细胞簇的微流体系统和方法，并且用于将粒子连续和自动封装一次分类为适合于这些排序的簇的尺寸的胶囊中的微流体系统和方法。 微流体系统包括其中蚀刻包括细胞分选单元的微通道阵列并且保护盖被接合在其上的基底，并且分选单元包括能够在其流动期间分离相对非粘性细胞簇的偏转装置， 尺寸范围从20μm到500μm和大约20到10000个细胞，例如胰岛的胰岛，在所述单元的出口处平行布置的至少两个分选微通道分别被设计成运输尽可能多的分类簇的类别 连续地到用于封装后者的单元，也形成在所述阵列中。

    Fluid content monitor
    29.
    发明授权
    Fluid content monitor 有权
    流体含量监测仪

    公开(公告)号：US08119068B2

    公开(公告)日：2012-02-21

    申请号：US12845190

    申请日：2010-07-28

    申请人： Rowan Connelly ,  Joel Leal

    发明人： Rowan Connelly ,  Joel Leal

    IPC分类号： G01N33/48

    CPC分类号： G01N1/14 ,  G01N21/251 ,  G01N33/18 ,  G01N2001/1093 ,  G01N2001/1454 ,  Y10S436/805 ,  Y10T436/11 ,  Y10T436/113332 ,  Y10T436/114165 ,  Y10T436/114998 ,  Y10T436/117497 ,  Y10T436/118339

    摘要： A fluid content monitor including a cuvette, a colorimeter adapted to generate a signal indicative of contents of a fluid sample contained in the cuvette, a container for holding a reagent, and a pump assembly for delivering reagent from the container to the cuvette. The pump assembly includes a tube extending from the container to the cuvette, check valves preventing reverse flow in the tube, and a hammer driven by a solenoid for repetitively compressing the tube to pump reagent to the cuvette. The cuvette can be removed for cleaning and replacement.

    摘要翻译： 一种流体含量监测器，包括比色皿，适于产生指示容纳在比色皿中的流体样品的内容物的信号的比色计，用于保持试剂的容器和用于将试剂从容器输送到比色皿的泵组件。 泵组件包括从容器延伸到比色皿的管，止回阀防止管中的反向流动，以及由螺线管驱动的锤，用于重复地压缩管以将试剂泵送到比色皿。 可以清洗比色皿进行清洁和更换。