公开(公告)号：US20080283799A1

公开(公告)日：2008-11-20

申请号：US12098337

申请日：2008-04-04

申请人： Jonathan S. Alden ,  Haixia Dai ,  Michael R. Knapp ,  Shuo Na ,  Hash Pakbaz ,  Florian Pschenitzka ,  Xina Quan ,  Michael A. Spaid ,  Adrian Winoto ,  Jeffrey Wolk

发明人： Jonathan S. Alden ,  Haixia Dai ,  Michael R. Knapp ,  Shuo Na ,  Hash Pakbaz ,  Florian Pschenitzka ,  Xina Quan ,  Michael A. Spaid ,  Adrian Winoto ,  Jeffrey Wolk

IPC分类号： H01B1/00

CPC分类号： H01B1/22 ,  B22F1/0025 ,  B22F2998/00 ,  B82Y20/00 ,  B82Y30/00 ,  C03C17/007 ,  C03C17/008 ,  C03C2217/445 ,  C03C2217/479 ,  C09D11/52 ,  C23F11/02 ,  C30B7/02 ,  C30B29/02 ,  C30B29/60 ,  C30B33/00 ,  G02F1/13439 ,  H01L27/14623 ,  H01L31/02164 ,  H01L31/022466 ,  H01L2224/45139 ,  H01L2224/49175 ,  H01L2924/00011 ,  H01L2924/12044 ,  H01L2924/3011 ,  H05K1/0269 ,  H05K1/097 ,  H05K3/048 ,  H05K2201/0145 ,  H05K2201/0154 ,  H05K2201/026 ,  H05K2201/10128 ,  H05K2203/1545 ,  Y10S977/762 ,  H01L2924/00 ,  H01L2924/01004 ,  H01L2924/0102 ,  H01L2924/01039

摘要： A transparent conductor including a conductive layer coated on a substrate is described. More specifically, the conductive layer comprises a network of nanowires which may be embedded in a matrix. The conductive layer is optically transparent and flexible. It can be coated or laminated onto a variety of substrates, including flexible and rigid substrates.

公开(公告)号：US07344865B2

公开(公告)日：2008-03-18

申请号：US11460550

申请日：2006-08-11

申请人： J. Wallace Parce ,  Theo T. Nikiforov ,  Tammy Burd Mehta ,  Anne R. Kopf-Sill ,  Andrea W. Chow ,  Michael R. Knapp

发明人： J. Wallace Parce ,  Theo T. Nikiforov ,  Tammy Burd Mehta ,  Anne R. Kopf-Sill ,  Andrea W. Chow ,  Michael R. Knapp

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/00

CPC分类号： C12Q1/6874 ,  B01F13/0059 ,  B01J2219/00317 ,  B01J2219/00459 ,  B01J2219/00468 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0487 ,  C07H21/02 ,  C07H21/04 ,  C12Q1/6869 ,  C40B60/14 ,  G01N27/44756 ,  G01N35/0098 ,  G01N2035/00574 ,  Y10T436/143333 ,  C12Q2565/629 ,  C12Q2535/101 ,  C12Q2563/173 ,  C12Q2525/186 ,  C12Q2527/125

摘要： Nucleotides and nucleotide analogs are used in various sequencing by incorporation/sequencing by synthesis methods. Nucleotide analogs comprising 3′-blocking groups are used to provide reversible chain-termination for sequencing by synthesis. Typical blocking groups include phosphate groups and carbamate groups. Fluorescent nucleotides are used to perform sequencing by synthesis with detection by incorporation of the fluorescently labeled nucleotide, optionally followed by photobleaching and intercalating dyes are used to detect addition of a non-labeled nucleotide in sequencing by synthesis with detection by intercalation. Microfluidic devices, including particle arrays, are used in the sequencing methods.

摘要翻译： 核苷酸和核苷酸类似物通过合成方法的并入/测序用于各种测序。 包含3'-封闭基团的核苷酸类似物用于提供可逆的链终止，用于通过合成进行测序。 典型的封闭基团包括磷酸基和氨基甲酸酯基。 使用荧光核苷酸进行测序，通过掺入荧光标记的核苷酸进行检测，任选地随后进行光漂白，并使用嵌入染料检测通过插入检测合成测序中的未标记核苷酸的添加。 微流控器件，包括粒子阵列，用于测序方法。

公开(公告)号：US06613513B1

公开(公告)日：2003-09-02

申请号：US09510205

申请日：2000-02-22

申请人： J. Wallace Parce ,  Theo T. Nikiforov ,  Tammy Burd Mehta ,  Anne R. Kopf-Sill ,  Andrea W. Chow ,  Michael R. Knapp

发明人： J. Wallace Parce ,  Theo T. Nikiforov ,  Tammy Burd Mehta ,  Anne R. Kopf-Sill ,  Andrea W. Chow ,  Michael R. Knapp

IPC分类号： C12Q168

CPC分类号： C12Q1/6874 ,  B01F13/0059 ,  B01J2219/00317 ,  B01J2219/00459 ,  B01J2219/00468 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0487 ,  C07H21/02 ,  C07H21/04 ,  C12Q1/6869 ,  C40B60/14 ,  G01N27/44756 ,  G01N35/0098 ,  G01N2035/00574 ,  Y10T436/143333 ,  C12Q2565/629 ,  C12Q2535/101 ,  C12Q2563/173 ,  C12Q2525/186 ,  C12Q2527/125

摘要： Nucleotides and nucleotide analogs are used in various sequencing by incorporation/sequencing by synthesis methods. Nucleotide analogs comprising 3′-blocking groups are used to provide reversible chain-termination for sequencing by synthesis. Typical blocking groups include phosphate groups and carbamate groups. Fluorescent nucleotides are used to perform sequencing by synthesis with detection by incorporation of the fluorescently labeled nucleotide, optionally followed by photobleaching and intercalating dyes are used to detect addition of a non-labeled nucleotide in sequencing by synthesis with detection by intercalation. Microfluidic devices, including particle arrays, are used in the sequencing methods.

公开(公告)号：US06447661B1

公开(公告)日：2002-09-10

申请号：US09416288

申请日：1999-10-12

申请人： Andrea W. Chow ,  Robert S. Dubrow ,  J. Wallace Parce ,  Steven A. Sundberg ,  Jeffrey A. Wolk ,  Ring-Ling Chien ,  Steven James Gallagher ,  Michael R. Knapp ,  Anne R. Kopf-Sill ,  Tammy Burd Mehta

发明人： Andrea W. Chow ,  Robert S. Dubrow ,  J. Wallace Parce ,  Steven A. Sundberg ,  Jeffrey A. Wolk ,  Ring-Ling Chien ,  Steven James Gallagher ,  Michael R. Knapp ,  Anne R. Kopf-Sill ,  Tammy Burd Mehta

IPC分类号： G01N2726

CPC分类号： B01L3/502715 ,  B01L3/50273 ,  B01L3/502746 ,  B01L2200/027 ,  B01L2300/0816 ,  B01L2300/0838 ,  B01L2300/0867 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0487 ,  B01L2400/084 ,  G01N2035/00237 ,  G01N2035/1062 ,  Y10S366/02 ,  Y10T436/2575

摘要： Methods, apparatus and systems are provided for introducing large numbers of different materials into a microfluidic analytical device rapidly, efficiently and reproducibly. In particular, improved integrated pipettor chip configurations, e.g. sippers or electropipettors, are described which are capable of sampling extremely small amounts of material for which analysis is desired, transporting material into a microfluidic analytical channel network, and performing the desired analysis on the material.

摘要翻译： 提供了方法，装置和系统，用于将大量不同的材料快速，有效和可再现地引入微流体分析装置。 特别地，改进的集成移液器芯片配置，例如 描述了能够对需要分析的极少量材料进行取样，将材料输送到微流体分析通道网络中并对材料进行所需分析的能力。

公开(公告)号：US6080295A

公开(公告)日：2000-06-27

申请号：US45339

申请日：1998-03-20

申请人： J. Wallace Parce ,  Michael R. Knapp

发明人： J. Wallace Parce ,  Michael R. Knapp

IPC分类号： G01N31/20 ,  B01F13/00 ,  B01J19/00 ,  B01L3/00 ,  B01L3/02 ,  F04B17/00 ,  F04B19/00 ,  G01N27/26 ,  G01N27/447 ,  G01N37/00 ,  H02N11/00

CPC分类号： B01F13/0071 ,  B01J19/0093 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502784 ,  F04B17/00 ,  F04B19/006 ,  G01N27/44704 ,  G01N27/44743 ,  G01N27/44791 ,  H02N11/006 ,  B01F13/0084 ,  B01F13/0086 ,  B01J2219/00891 ,  B01L2200/027 ,  B01L2400/0415 ,  B01L2400/0418 ,  B01L2400/0421 ,  B01L3/021 ,  Y10S366/02 ,  Y10T436/113332 ,  Y10T436/2575

摘要： The present invention provides for techniques for transporting materials using electrokinetic forces through the channels of a microfluidic system. The subject materials are transported in regions of high ionic concentration, next to spacer material regions of high ionic concentration, which are separated by spacer material regions of low ionic concentration. Such arrangements allow the materials to remain localized for the transport transit time to avoid mixing of the materials. Using these techniques, an electropipettor which is compatible with the microfluidic system is created so that materials can be easily introduced into the microfluidic system. The present invention also compensates for electrophoretic bias as materials are transported through the channels of the microfluidic system by splitting a channel into portions with positive and negative surface charges and a third electrode between the two portions, or by diffusion of the electrophoresing materials after transport along a channel.

公开(公告)号：US09899123B2

公开(公告)日：2018-02-20

申请号：US12098329

申请日：2008-04-04

申请人： Jonathan S. Alden ,  Haixia Dai ,  Michael R. Knapp ,  Shuo Na ,  Hash Pakbaz ,  Florian Pschenitzka ,  Xina Quan ,  Michael A. Spaid ,  Adrian Winoto ,  Jeffrey Wolk

发明人： Jonathan S. Alden ,  Haixia Dai ,  Michael R. Knapp ,  Shuo Na ,  Hash Pakbaz ,  Florian Pschenitzka ,  Xina Quan ,  Michael A. Spaid ,  Adrian Winoto ,  Jeffrey Wolk

IPC分类号： B05D5/12 ,  H01B1/22 ,  B82Y20/00 ,  B82Y30/00 ,  C09D11/52 ,  C23F11/02 ,  C30B7/02 ,  C30B29/02 ,  C30B29/60 ,  C30B33/00 ,  G02F1/1343 ,  H01L31/0224 ,  C03C17/00 ,  H01L27/146 ,  H01L31/0216 ,  H05K1/02 ,  H05K1/09 ,  H05K3/04

CPC分类号： H01B1/22 ,  B22F1/0025 ,  B22F2998/00 ,  B82Y20/00 ,  B82Y30/00 ,  C03C17/007 ,  C03C17/008 ,  C03C2217/445 ,  C03C2217/479 ,  C09D11/52 ,  C23F11/02 ,  C30B7/02 ,  C30B29/02 ,  C30B29/60 ,  C30B33/00 ,  G02F1/13439 ,  H01L27/14623 ,  H01L31/02164 ,  H01L31/022466 ,  H01L2224/45139 ,  H01L2224/49175 ,  H01L2924/00011 ,  H01L2924/12044 ,  H01L2924/3011 ,  H05K1/0269 ,  H05K1/097 ,  H05K3/048 ,  H05K2201/0145 ,  H05K2201/0154 ,  H05K2201/026 ,  H05K2201/10128 ,  H05K2203/1545 ,  Y10S977/762 ,  H01L2924/00 ,  H01L2924/01004 ,  H01L2924/0102 ,  H01L2924/01039

摘要： A method for forming a transparent conductor including a conductive layer coated on a substrate is described. The method comprises depositing a plurality of metal nanowires on a surface of a substrate, the metal nanowires being dispersed in a liquid; and forming a metal nanowire network layer on the substrate by allowing the liquid to dry.

公开(公告)号：US09101928B2

公开(公告)日：2015-08-11

申请号：US13015242

申请日：2011-01-27

申请人： Tammy Burd Mehta ,  Anne R. Kopf-Sill ,  J. Wallace Parce ,  Andrea W. Chow ,  Luc J. Bousse ,  Michael R. Knapp ,  Theo T. Nikiforov ,  Steve Gallagher

发明人： Tammy Burd Mehta ,  Anne R. Kopf-Sill ,  J. Wallace Parce ,  Andrea W. Chow ,  Luc J. Bousse ,  Michael R. Knapp ,  Theo T. Nikiforov ,  Steve Gallagher

IPC分类号： G01N35/00 ,  B01L3/00 ,  C07H21/02 ,  C07H21/04 ,  C12Q1/68 ,  B01F13/00 ,  C40B60/14 ,  G01N27/447

CPC分类号： C12Q1/6874 ,  B01F13/0059 ,  B01J2219/00317 ,  B01J2219/00459 ,  B01J2219/00468 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0487 ,  C07H21/02 ,  C07H21/04 ,  C12Q1/6869 ,  C40B60/14 ,  G01N27/44756 ,  G01N35/0098 ,  G01N2035/00574 ,  Y10T436/143333 ,  C12Q2565/629 ,  C12Q2535/101 ,  C12Q2563/173 ,  C12Q2525/186 ,  C12Q2527/125

摘要： An array of transportable particle sets is used in a microfluidic device for performing chemical reactions in the microfluidic device. The microfluidic device comprises a main channel and intersecting side channels, the main channel and side channels forming a plurality of intersections. The array of particle sets is disposed in the main channel, and the side channels are coupled to reagents. As the particle sets are transported through the intersections of the main channel and the side channels, reagents are flowed through the side channels into contact with each array member (or selected array members), thereby providing a plurality of chemical reactions in the microfluidic system.

摘要翻译： 在微流体装置中使用可移动颗粒组的阵列，用于在微流体装置中执行化学反应。 微流体装置包括主通道和相交的侧通道，主通道和侧通道形成多个交叉点。 颗粒组的阵列设置在主通道中，并且侧通道与试剂偶联。 当颗粒组通过主通道和侧通道的交叉点传输时，试剂通过侧通道流动与每个阵列构件（或选定的阵列构件）接触，从而在微流体系统中提供多个化学反应。

公开(公告)号：US08865027B2

公开(公告)日：2014-10-21

申请号：US12098337

申请日：2008-04-04

申请人： Jonathan S. Alden ,  Haixia Dai ,  Michael R. Knapp ,  Shuo Na ,  Hash Pakbaz ,  Florian Pschenitzka ,  Xina Quan ,  Michael A. Spaid ,  Adrian Winoto ,  Jeffrey Wolk

发明人： Jonathan S. Alden ,  Haixia Dai ,  Michael R. Knapp ,  Shuo Na ,  Hash Pakbaz ,  Florian Pschenitzka ,  Xina Quan ,  Michael A. Spaid ,  Adrian Winoto ,  Jeffrey Wolk

IPC分类号： H01B1/02 ,  H01B1/20 ,  C30B29/02 ,  H01B1/22 ,  B82Y20/00 ,  C30B29/60 ,  B82Y30/00 ,  C30B7/02 ,  G02F1/1343 ,  H01L31/0224 ,  C09D11/52 ,  C23F11/02 ,  C30B33/00 ,  H01L31/0216 ,  H01L27/146

CPC分类号： H01B1/22 ,  B22F1/0025 ,  B22F2998/00 ,  B82Y20/00 ,  B82Y30/00 ,  C03C17/007 ,  C03C17/008 ,  C03C2217/445 ,  C03C2217/479 ,  C09D11/52 ,  C23F11/02 ,  C30B7/02 ,  C30B29/02 ,  C30B29/60 ,  C30B33/00 ,  G02F1/13439 ,  H01L27/14623 ,  H01L31/02164 ,  H01L31/022466 ,  H01L2224/45139 ,  H01L2224/49175 ,  H01L2924/00011 ,  H01L2924/12044 ,  H01L2924/3011 ,  H05K1/0269 ,  H05K1/097 ,  H05K3/048 ,  H05K2201/0145 ,  H05K2201/0154 ,  H05K2201/026 ,  H05K2201/10128 ,  H05K2203/1545 ,  Y10S977/762 ,  H01L2924/00 ,  H01L2924/01004 ,  H01L2924/0102 ,  H01L2924/01039

摘要： A transparent conductor including a conductive layer coated on a substrate is described. More specifically, the conductive layer comprises a network of nanowires which may be embedded in a matrix. The conductive layer is optically transparent and flexible. It can be coated or laminated onto a variety of substrates, including flexible and rigid substrates.

摘要翻译： 描述了包括涂覆在基板上的导电层的透明导体。 更具体地，导电层包括可嵌入矩阵中的纳米线网络。 导电层是光学透明和柔性的。 它可以涂覆或层压在各种基材上，包括柔性和刚性基材。

公开(公告)号：US20120288865A1

公开(公告)日：2012-11-15

申请号：US13444502

申请日：2012-04-11

申请人： Steven A. Sundberg ,  Michael R. Knapp ,  Ivor T. Knight ,  Deborah J. Boles ,  Aaron J. Rulison ,  Wesley B. Dong ,  Edward P. Donlon ,  Robert J. Moti ,  Andrew G. Fabans ,  Allen R. Boronkay ,  Michael Slater

发明人： Steven A. Sundberg ,  Michael R. Knapp ,  Ivor T. Knight ,  Deborah J. Boles ,  Aaron J. Rulison ,  Wesley B. Dong ,  Edward P. Donlon ,  Robert J. Moti ,  Andrew G. Fabans ,  Allen R. Boronkay ,  Michael Slater

IPC分类号： G01N25/04

CPC分类号： G01N25/04 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/50273 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/10 ,  B01L2300/0627 ,  B01L2300/0816 ,  B01L2300/14 ,  B01L2300/18 ,  B01L2300/1833 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/0487 ,  C12Q1/68 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q1/6862 ,  G01N21/33 ,  G01N21/6428 ,  Y10T436/143333 ,  C12Q2565/629 ,  C12Q2565/101 ,  C12Q2527/107 ,  C12Q2527/101

摘要： The present invention provides novel methods and devices that employ microfluidic technology to generate molecular melt curves. In particular, the devices and methods in accordance with the invention are useful in providing for the analysis of PCR amplification products.

摘要翻译： 本发明提供了采用微流体技术产生分子熔融曲线的新颖方法和装置。 特别地，根据本发明的装置和方法可用于提供PCR扩增产物的分析。

公开(公告)号：US08275554B2

公开(公告)日：2012-09-25

申请号：US12684601

申请日：2010-01-08

申请人： Michael R. Knapp ,  Jill M. Baker ,  Andrea W. Chow ,  Anne R. Kopf-Sill ,  Michael A. Spaid

发明人： Michael R. Knapp ,  Jill M. Baker ,  Andrea W. Chow ,  Anne R. Kopf-Sill ,  Michael A. Spaid

IPC分类号： G01N33/48

CPC分类号： C12Q1/686 ,  B01L3/5027 ,  B01L7/52 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/1827 ,  B01L2400/0406 ,  C12Q1/6837 ,  C12Q1/6851 ,  C12Q2531/113 ,  C12Q2565/629 ,  C12Q2527/143 ,  C12Q2547/107 ,  C12Q2537/157

摘要： Systems for differentiating the lengths of nucleic acids of interest in a sample are provided. The system includes a microfluidic device, a detector, and a software system. The microfluidic device includes an amplification microchannel or microchamber containing a reaction mixture under conditions that provide one or more amplicons of the nucleic acid of interest. The detector is integral with or proximal to the microfluidic device and is configured to detect the amplicons as one or more signals from a homogenous mixture. The software system interprets one or more coincidentally detected signals to indicate lengths of one or more individual nucleic acid molecules of interest, thereby differentiating the lengths of the nucleic acids of interest.

摘要翻译： 提供了用于区分样品中感兴趣的核酸长度的系统。 该系统包括微流体装置，检测器和软件系统。 微流体装置包括在提供感兴趣的核酸的一个或多个扩增子的条件下含有反应混合物的扩增微通道或微室。 检测器与微流体装置成一体或靠近微流体装置，并且被配置为将扩增子检测为来自均匀混合物的一个或多个信号。 软件系统解释一个或多个巧合检测的信号以指示感兴趣的一个或多个单个核酸分子的长度，从而区分感兴趣的核酸的长度。