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88 条记录 (耗时 0.031 秒)

    Apparatus and method for dilution and mixing of liquid samples
    65.
    发明授权
    Apparatus and method for dilution and mixing of liquid samples 失效
    液体样品稀释和混合的装置和方法

    公开(公告)号：US4868129A

    公开(公告)日：1989-09-19

    申请号：US90026

    申请日：1987-08-27

    申请人： Ian Gibbons ,  Robert S. Hillman ,  Channing R. Robertson

    发明人： Ian Gibbons ,  Robert S. Hillman ,  Channing R. Robertson

    IPC分类号： B01F13/00 ,  B01F13/08 ,  B01F15/04 ,  G01N1/38 ,  G01N33/04

    CPC分类号： G01N1/38 ,  B01F13/0059 ,  B01F13/0818 ,  B01F15/04 ,  B01L3/5027 ,  G01N33/04 ,  Y10T436/25625 ,  Y10T436/2575

    摘要： An apparatus for diluting a sample with a diluent, comprising a fixed volume measuring chamber, a fixed volume receiving chamber and fluid receiving relationship to the measuring chamber, a gas vent in the receiving chamber, a stop flow junction between the measuring chamber and the receiving chamber, a sample application site in fluid donating relationship to the measuring chamber, wherein the vertical height difference between the sample application site and the stop flow junction is insufficient to provide a hydrostatic pressure capable of overcoming backpressure at the stop flow junction when sample is applied to the sample application site, and the diluent application site and fluid donating relationship to the measuring chamber. Sample and diluent are added sequentially to the apparatus of the invention without requiring any intervening operations. Various means are provided for restarting flow, which causes diluent to wash the sample into the receiving chamber, where the two can be mixed.

    摘要翻译： 一种用稀释剂稀释样品的装置，包括固定容积测量室，固定容积接收室和与测量室的流体接收关系，接收室中的气体通道，测量室和接收器之间的停止流动接合 室，与测量室的流体供应关系的样品施加部位，其中样品施加部位和止动流动接合点之间的垂直高度差不足以提供当施加样品时能够克服止动流动接合处的背压的静水压力 到样品应用场所，以及稀释剂施用部位和与测量室的流体供给关系。 样品和稀释剂依次添加到本发明的装置中，而不需要任何中间操作。 提供了重新启动流动的各种装置，这使得稀释剂将样品洗涤到接收室中，其中两个可以混合。

    Enzymatic poly-reactant channeling binding assay
    66.
    发明授权
    Enzymatic poly-reactant channeling binding assay 失效
    酶多反应物通道结合测定

    公开(公告)号：US4687735A

    公开(公告)日：1987-08-18

    申请号：US474906

    申请日：1983-03-14

    申请人： Robert K. DiNello ,  Ian Gibbons ,  Edwin F. Ullman

    发明人： Robert K. DiNello ,  Ian Gibbons ,  Edwin F. Ullman

    IPC分类号： G01N33/543 ,  C12Q1/00 ,  C12Q1/54 ,  G01N33/535 ,  G01N33/536 ,  G01N33/542 ,  G01N33/58

    CPC分类号： G01N33/542 ,  G01N33/535 ,  G01N33/581 ,  Y10S435/81 ,  Y10S435/966

    摘要： Improved sensitive immunoassays are provided involving channeling involving one, usually two enzymes, where the enzymes are related by the product of one enzyme being the substrate of the other enzyme. A dispersed aggregation is formed in the assay medium of (1) the analyte, (2) one of the enzymes bound to a second binding member ("SBM") (enzyme - SBM conjugate) which conjugate is non-covalently bound to a first binding member ("FBM"), and (3) a multiplied amount of the other enzyme bound in the complex. The large amount of enzyme or reactant in the complex is achieved by having a multiplicity of linkages binding the enzyme or reactant directly or indirectly to FBMs. The enzyme channeling provides for a detectable signal which can be related to the amount of analyte in the medium.

    摘要翻译： 提供了改进的敏感性免疫测定法，其涉及涉及一种通常两种酶的引导，其中酶与另一种酶的底物的酶的产物相关。 在（1）分析物的测定培养基中形成分散的聚集体，（2）与第二结合成员（“SBM”）结合的酶之一（酶-SMM缀合物），其结合物与第一结合物非共价结合 结合成员（“FBM”），和（3）复合物中结合的另一种酶的量。 复合物中大量的酶或反应物通过具有将酶或反应物直接或间接地结合到FBM的多重连接来实现。 酶通道提供可以与介质中分析物的量相关的可检测信号。

    Double antibody for enhanced sensitivity in immunoassay
    67.
    发明授权
    Double antibody for enhanced sensitivity in immunoassay 失效
    双抗体，增强免疫测定的灵敏度

    公开(公告)号：US4281061A

    公开(公告)日：1981-07-28

    申请号：US61542

    申请日：1979-07-27

    申请人： Robert F. Zuk ,  Ian Gibbons ,  Gerald L. Rowley ,  Edwin F. Ullman

    发明人： Robert F. Zuk ,  Ian Gibbons ,  Gerald L. Rowley ,  Edwin F. Ullman

    IPC分类号： C12N9/96 ,  G01N33/542 ,  G01N31/14 ,  G01N33/48 ,  G01N33/54

    CPC分类号： G01N33/542 ,  C12N9/96 ,  Y10S435/966 ,  Y10S435/968 ,  Y10S435/971 ,  Y10S436/80 ,  Y10S436/808

    摘要： Method and compositions are provided for performing homogeneous immunoassays. The method involves having a signal producing system, which provides a detectable signal, which system includes a macromolecular member. The determination of the analyte, which is a member of a specific binding pair consisting of a ligand and its homologous receptor, is performed by creating an extensive matrix in the assay medium by having in the assay medium in addition to the analyte, ligand labeled with one of the members of the signal producing system, antiligand either present as the analyte or added, a polyvalent receptor for antiligand, the macromolecular member of the signal producing system, and any additional members of the signal producing system. The labeled ligand, antiligand, and polyvalent receptor for the antiligand create a matrix which modulates, e.g. inhibits, the approach of the macromolecular member of the signal producing system to the labeled ligand. The extent and degree of formation of the matrix is dependent upon the concentration of the analyte in the medium. By comparing the signal from an assay medium having an unknown amount of analyte, with a signal obtained from an assay medium having a known amount of analyte, the amount of analyte in the unknown sample may be determined qualitatively or quantitatively.Kits are provided having predetermined amounts of the various reagents to allow for enhanced sensitivity of the method.

    摘要翻译： 提供方法和组合物用于进行均匀的免疫测定。 该方法包括具有提供可检测信号的信号产生系统，该系统包括大分子成员。 由配体及其同源受体组成的特异性结合对成员的分析物的测定通过在测定培养基中通过在测定培养基中除了分析物之外产生广泛的基质来进行，所述分析物被标记有 信号生成系统的成员之一，反配位体作为分析物存在或添加，反配体的多价受体，信号产生系统的大分子成员以及信号产生系统的任何附加成员。 用于反配体的标记的配体，反配体和多价受体产生调节的基质。 抑制信号产生系统的大分子成员对标记的配体的接近。 基质的形成程度取决于培养基中分析物的浓度。 通过比较来自具有未知量分析物的测定培养基的信号与从具有已知量分析物的测定培养基获得的信号，可以定性或定量地确定未知样品中分析物的量。 提供具有预定量的各种试剂以提供该方法的增强灵敏度的试剂盒。