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    Apparatus and method for polymer synthesis using arrays
    133.
    发明申请
    Apparatus and method for polymer synthesis using arrays 审中-公开
    使用阵列进行聚合物合成的装置和方法

    公开(公告)号：US20030069411A1

    公开(公告)日：2003-04-10

    申请号：US10171497

    申请日：2002-06-12

    发明人： Thomas M. Brennan

    IPC分类号： C07H021/04 ,  C12M001/34 ,  B32B027/12

    CPC分类号： C07H21/00 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/0031 ,  B01J2219/00313 ,  B01J2219/00315 ,  B01J2219/00328 ,  B01J2219/00364 ,  B01J2219/00369 ,  B01J2219/00414 ,  B01J2219/00416 ,  B01J2219/00418 ,  B01J2219/00423 ,  B01J2219/00454 ,  B01J2219/00459 ,  B01J2219/005 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00686 ,  B01J2219/00689 ,  B01J2219/00695 ,  B01J2219/0072 ,  B01J2219/00722 ,  B01J2219/00725 ,  C07B2200/11 ,  C07K1/04 ,  C07K1/045 ,  C40B40/06 ,  C40B40/10 ,  C40B50/14 ,  C40B60/14 ,  G01N35/1072

    摘要： A polymer synthesis apparatus (20) for building a polymer chain including a head assembly (21) having an array of nozzles (22) with each nozzle coupled to a reservoir (23) of liquid reagent (24), and a base assembly (25) having an array of reaction wells (26). A transport mechanism (27) aligns the reaction wells (26) and selected nozzles (22) for deposition of the liquid reagent (24) into selected reaction wells (26). A sliding seal (30) is positioned between the head assembly (21) and the base assembly (25) to form a common chamber (31) enclosing both the reaction well (26) and the nozzles (22) therein. A gas inlet (70) into the common chamber (31), upstream from the nozzles (22), and a gas outlet (71) out of the common chamber (31), downstream from the nozzles (22), sweeps the common chamber (31) of toxic fumes emitted by the reagents. Each reaction well (26) includes an orifice (74) extending into the well (26) which is of a size and dimension to form a capillary liquid seal to retain the reagent solution (76) in the well (26) for polymer chain growth therein. A pressure regulating device (82) is provided for controlling a pressure differential, between a first gas pressure exerted on the reaction well (26) and a second gas pressure exerted on an exit (80) of the orifice, such that upon the pressure differential exceeding a predetermined amount, the reagent solution (76) is expelled from the well (26) through the orifice (74). A method of synthesis of a polymer chain in a synthesis apparatus (20) is also included.

    摘要翻译： 一种用于构建聚合物链的聚合物合成设备（20），其包括具有喷嘴阵列（22）的头组件（21），每个喷嘴连接到液体试剂（24）的容器（23），以及基座组件 ）具有反应孔阵列（26）。 输送机构（27）对准反应孔（26）和选定的喷嘴（22），用于将液体试剂（24）沉积到选定的反应孔（26）中。 滑动密封件（30）定位在头部组件（21）和基座组件（25）之间，以形成在其中包围反应井（26）和喷嘴（22）的公共腔室（31）。 从喷嘴（22）的上游进入公共室（31）的气体入口（70）和在公共室（31）之外的喷嘴（22）的下游的气体出口（71）扫过公共室 （31）试剂发出的有毒烟雾。 每个反应井（26）包括延伸到井（26）中的孔（74），其具有尺寸和尺寸以形成毛细管液体密封，以将试剂溶液（76）保持在井（26）中用于聚合物链增长 其中。 提供压力调节装置（82），用于控制施加在反应井（26）上的第一气体压力和施加在孔口出口（80）上的第二气体压力之间的压力差，使得在压差 超过预定量的试剂溶液（76）通过孔口（74）从孔26排出。 也包括在合成装置（20）中合成聚合物链的方法。

    Method and apparatus based on bundled capillaries for high throughput screening
    136.
    发明申请
    Method and apparatus based on bundled capillaries for high throughput screening 审中-公开
    基于捆绑毛细管的方法和装置，用于高通量筛选

    公开(公告)号：US20020164824A1

    公开(公告)日：2002-11-07

    申请号：US10080274

    申请日：2002-02-19

    发明人： Jianming Xiao ,  Shiping Chen ,  Yuling Luo ,  John Butler ,  Masoud Qazan ,  Jochen Thiele ,  Anthony Chen

    IPC分类号： G01N033/545 ,  C12M003/00

    CPC分类号： G01N21/648 ,  B01J19/0046 ,  B01J2219/00369 ,  B01J2219/00382 ,  B01J2219/00385 ,  B01J2219/00418 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00585 ,  B01J2219/00587 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00619 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00707 ,  B01J2219/00722 ,  B01J2219/00725 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  G01N21/6452 ,  G01N2021/6484

    摘要： Disclosed are systems and methods involved in extreme high throughput screening of compounds which have an affinity for a biological target. The system is based on a capillary bundle with two distinguishable ends wherein capillaries on one end are connected to compounds stored in discrete reservoirs and capillaries on the other end are bound and processed to form a two dimensional microarray. A capillary bundle having reaction wells for hybridization and compound reaction in one end of the capillaries is disclosed. The capillaries may be light-conducting. Also disclosed are various methods of identifying a target compound in a liquid using this capillary bundle as well as methods of fabricating the bundle. A novel surface tension guided reaction chamber is also provided. Methods and chemistry for fabrication and use of a surface tension guided reaction chamber in binding and hybridization assays are also disclosed. Methods and systems for precise metering of fluids within the capillaries and at the reaction chambers, including the surface tension guided nullvirtualnull reaction well is provided. Methods for performing high throughput screens using optical fiber lined capillaries of the invention are also provided. The capillary arrays are used with both liquid probes as well as probes immobilized on the walls of the capillary.

    摘要翻译： 公开了涉及对生物靶具有亲和力的化合物的极高通量筛选中涉及的系统和方法。 该系统基于毛细管束，其具有两个可区分的端部，其中一端的毛细管连接到存储在离散储存器中的化合物，另一端的毛细血管被结合并加工以形成二维微阵列。 公开了一种毛细管束，其具有在毛细血管的一端中用于杂交和化合物反应的反应孔。 毛细血管可能是导光的。 还公开了使用该毛细管束在液体中鉴定目标化合物的各种方法以及制造束的方法。 还提供了一种新颖的表面张力引导反应室。 还公开了在结合和杂交测定中制造和使用表面张力引导反应室的方法和化学。 提供了用于精确计量毛细管内和反应室内的流体的方法和系统，包括表面张力引导的“虚拟”反应井。 还提供了使用本发明的光纤衬里毛细管进行高通量筛选的方法。 毛细管阵列与两个液体探针以及固定在毛细管壁上的探针一起使用。

    Sample deposition method and system
    139.
    发明申请
    Sample deposition method and system 失效
    样品沉积方法和系统

    公开(公告)号：US20020092366A1

    公开(公告)日：2002-07-18

    申请号：US09765207

    申请日：2001-01-17

    发明人： Ansgar Brock ,  Christopher M. Shaw ,  Robert C. Downs

    IPC分类号： G01N001/10

    CPC分类号： B01L3/0268 ,  B01J2219/00369 ,  B01J2219/00371 ,  B01J2219/00653 ,  B01J2219/00691 ,  B01L2300/0838 ,  B01L2400/027 ,  C40B60/14 ,  Y10T436/2575

    摘要： A method and system to deposit rapidly, accurately and efficiently liquid droplets onto a sample plate. A liquid from which the droplet is formed contains sample molecules, matrix molecules or a mixture of both sample and matrix molecules. A sample plate is positioned with respect to a position below a portion of the liquid, such as a droplet. A power supply forms an electric field between the sample plate and the liquid, which pulls the droplet to a target location on the sample plate. A plurality of capillary columns is provided for simultaneous or successive multiple depositions via application of one or more high voltage pulses between the liquid sample array and a sample plate.

    摘要翻译： 一种快速，准确和有效地将液滴沉积在样品板上的方法和系统。 形成液滴的液体包含样品分子，基质分子或样品和基质分子的混合物。 样品板相对于液体的一部分下方的位置（例如液滴）定位。 电源在样品板和液体之间形成电场，其将液滴拉到样品板上的目标位置。 提供多个毛细管柱，用于通过在液体样品阵列和样品板之间施加一个或多个高电压脉冲同时或连续多次沉积。