公开(公告)号：US11896969B2

公开(公告)日：2024-02-13

申请号：US17012108

申请日：2020-09-04

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： Christopher Belisle ,  Xuemei He ,  Hong Chen ,  Yueping Xu ,  Jiali Liao

IPC: B01J41/07 ,  B01J47/02 ,  C07K1/18 ,  C12N9/88 ,  B01J47/014 ,  B01J41/14

CPC classification number: B01J41/14 ,  B01J41/07 ,  B01J47/014 ,  B01J47/02 ,  C07K1/18 ,  C12N9/88 ,  C12Y402/01045 ,  B01J2220/52

Abstract: Chromatography resins having anionic exchange-hydrophobic mixed mode ligands and methods of using such resins are provided.

公开(公告)号：US20240024870A1

公开(公告)日：2024-01-25

申请号：US18229545

申请日：2023-08-02

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Joshua RITCHEY ,  Yi XUE ,  Robert MELTZER

IPC: B01L3/00 ,  C09D183/04 ,  C09D183/00 ,  B81C1/00

CPC classification number: B01L3/50273 ,  C09D183/04 ,  C09D183/00 ,  B81C1/00206 ,  B01L3/502707 ,  B01L3/502784 ,  B01L2300/165 ,  C12Q1/686

Abstract: Compositions, devices, and methods are disclosed for the modification of polymer surfaces with coatings having a dispersion of silicone polymer and hydrophobic silica. The surface coatings provide the polymer surface with high hydrophobicity, as well as increased resistance to biofouling with proteinaceous material. The polymer surfaces can be particularly useful in microfluidic devices and methods that involve the contacting of the covalently modified polymer surfaces with emulsions of aqueous droplets containing biological macromolecules within an oil carrier phase.

公开(公告)号：US11878302B2

公开(公告)日：2024-01-23

申请号：US17465763

申请日：2021-09-02

Applicant: Bio-Rad Laboratories, Inc.

Inventor： George Carman ,  Thomas H. Cauley, III ,  David P. Stumbo

IPC: B01L3/00

CPC classification number: B01L3/502784 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2400/0487

Abstract: System, including methods and apparatus, for spacing droplets from each other and for detection of spaced droplets.

公开(公告)号：US11866771B2

公开(公告)日：2024-01-09

申请号：US17345834

申请日：2021-06-11

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Amy L. Hiddessen ,  Benjamin J. Hindson

IPC: C12Q1/6848 ,  C12Q1/6844 ,  C12Q1/6816 ,  C12Q1/686

CPC classification number: C12Q1/6848 ,  C12Q1/686 ,  C12Q1/6816 ,  C12Q1/6846 ,  C12Q1/6846 ,  C12Q1/6846 ,  C12Q2527/125 ,  C12Q2563/159 ,  C12Q1/6848 ,  C12Q1/6846 ,  C12Q2527/125 ,  C12Q2563/159

Abstract: System, including methods, apparatus, compositions, and kits, for making and using a stabilized emulsion. A method of generating a stabilized emulsion is provided. In the method, an aqueous phase may be provided. The aqueous phase may include an effective concentration of one or more skin-forming proteins. An emulsion may be formed. The emulsion may include droplets of a dispersed phase disposed in a continuous phase, with the aqueous phase being the continuous phase or the dispersed phase. The emulsion may be heated to create an interfacial skin between each droplet and the continuous phase, to transform the droplets into capsules.

公开(公告)号：US20230416805A1

公开(公告)日：2023-12-28

申请号：US18209350

申请日：2023-06-13

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Rahul RATTAN ,  Marta GONZALEZ-PLASKY

IPC: C12Q1/6816

CPC classification number: C12Q1/6816

Abstract: Methods of decreasing bead aggregation and improving specificity of nucleic acid hybridization using non-specific nucleic acid binding proteins is described.

公开(公告)号：US20230408505A1

公开(公告)日：2023-12-21

申请号：US18354144

申请日：2023-07-18

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Michael L. Samuels ,  Darren Roy Link

IPC: G01N33/543 ,  C40B40/04 ,  C40B70/00 ,  G01N33/532 ,  C40B40/00 ,  C12Q1/6874

CPC classification number: G01N33/54366 ,  C40B40/04 ,  C40B70/00 ,  G01N33/532 ,  C40B40/00 ,  C12Q1/6874 ,  B01F33/3011

Abstract: The invention generally relates to performing sandwich assays in droplets. In certain embodiments, the invention provides methods for detecting a target analyte that involve forming a compartmentalized portion of fluid including a portion of a sample suspected of containing a target analyte and a sample identifier, a first binding agent having a target identifier, and a second binding agent specific to the target analyte under conditions that produce a complex of the first and second binding agents with the target analyte, separating the complexes, and detecting the complexes, thereby detecting the target analyte.

公开(公告)号：US20230407372A1

公开(公告)日：2023-12-21

申请号：US18242658

申请日：2023-09-06

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  John Stark ,  Yadwinder Deol ,  Vishal Sharma

IPC: C12Q1/6811 ,  C12Q1/6874 ,  C12N15/10

CPC classification number: C12Q1/6811 ,  C12Q1/6874 ,  C12Q2563/179 ,  C12Q2565/629 ,  C12Q2600/178 ,  C12N15/1065

Abstract: A system and method for target material capture, the method comprising: receiving a set of target cells into an array of wells defined at a surface plane of a substrate; receiving a set of particles into the array of wells, thereby co-capturing the set of target cells and the set of particles; achieving a desired state for the array of wells upon receiving a washing fluid into a cavity in communication with the array of wells; receiving a lysis buffer into the cavity; receiving a partitioning fluid into the cavity, thereby displacing the lysis buffer from the cavity and partitioning each of the array of wells from adjacent wells, at the surface plane; and retaining intercellular material of the set of target cells, individually with the set of particles within the array of wells.

公开(公告)号：US20230393069A1

公开(公告)日：2023-12-07

申请号：US18448631

申请日：2023-08-11

Applicant: Bio-Rad Laboratories, inc.

Inventor： Darren Roy Link ,  Michael L. Samuels

IPC: G01N21/64 ,  B01J19/00 ,  B01L3/00 ,  C40B40/04 ,  C40B50/08 ,  C40B60/10 ,  G01N33/50 ,  C12Q1/6851 ,  B01F23/41 ,  B01F25/433 ,  B01F33/3011 ,  C12Q1/25 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/6827 ,  G01N33/542 ,  G01N33/573 ,  G01N33/58

CPC classification number: G01N21/6445 ,  B01J19/0046 ,  B01L3/502761 ,  C40B40/04 ,  C40B50/08 ,  C40B60/10 ,  G01N33/5008 ,  C12Q1/6851 ,  B01F23/41 ,  B01F25/433 ,  B01F25/4336 ,  B01F33/3011 ,  C12Q1/25 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/6827 ,  G01N33/542 ,  G01N33/573 ,  G01N33/582 ,  B01L7/52

Abstract: The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number.

公开(公告)号：US11834655B2

公开(公告)日：2023-12-05

申请号：US17565249

申请日：2021-12-29

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ronald Lebofsky ,  Jeremy Agresti

IPC: C12N5/10 ,  C12Q1/6806 ,  C12N15/10 ,  C12Q1/6869 ,  C12Q1/6809

CPC classification number: C12N15/1065 ,  C12Q1/6806 ,  C12Q1/6809 ,  C12Q1/6869 ,  C12Q2563/185 ,  C12Q1/6869 ,  C12Q1/6855 ,  C12Q1/6806 ,  C12Q1/6855

Abstract: Methods and compositions are provided for making and using uniquely tagged target nucleic acid molecules.

公开(公告)号：US20230364614A1

公开(公告)日：2023-11-16

申请号：US18227212

申请日：2023-07-27

Applicant: Bio-Rad Laboratories, Inc. ,  Bio-Rad Europe GmbH

Inventor： Iago Pereiro Pereiro ,  Anna Fomitcheva Khartchenko ,  Robert Dean Lovchik ,  Govind Kaigala ,  Lorenzo Franco Teodoro Petrini ,  Anel Zulji ,  Frédéric M. Buffière ,  Johann Guégan ,  Eliane Rivalin ,  Sylvie Villard-Saussine

IPC: B01L3/00

CPC classification number: B01L3/502784 ,  B01L3/502746 ,  B01L2200/027 ,  B01L2300/0832 ,  B01L2400/0487 ,  B01L2400/0622

Abstract: In one implementation, a microfluidic probe has a non-planar processing surface and an inlet aperture. The shape of the surface may be selected to produce a specific velocity gradient profile across a surface onto which fluid is deposited using the microfluidic probe, for example a constant velocity gradient or a velocity gradient that decreases linearly with distance from the inlet aperture. The microfluidic probe may define and overflow notch in a perimeter edge of the processing surface.