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    QUANTITATIVE PCR-BASED METHOD TO PREDICT THE EFFICIENCY OF TARGET ENRICHMENT FOR NEXT-GENERATION SEQUENCING USING REPETITIVE DNA ELEMENTS (LINES/SINES) AS NEGATIVE CONTROLS
    34.
    发明申请
    QUANTITATIVE PCR-BASED METHOD TO PREDICT THE EFFICIENCY OF TARGET ENRICHMENT FOR NEXT-GENERATION SEQUENCING USING REPETITIVE DNA ELEMENTS (LINES/SINES) AS NEGATIVE CONTROLS 有权
    使用基于PCR的方法来预测目标增强的效率，使用重复的DNA元素（线/新）作为负向控制进行下一步序列测序

    公开(公告)号：US20130137582A1

    公开(公告)日：2013-05-30

    申请号：US13308482

    申请日：2011-11-30

    申请人： Joseph Hoang Hai Ong ,  Scott Happe

    发明人： Joseph Hoang Hai Ong ,  Scott Happe

    IPC分类号： C40B10/00

    CPC分类号： C12Q1/6806 ,  C12Q1/6869 ,  C12Q2537/159 ,  C12Q2545/101

    摘要： A method for determining an efficiency of target enrichment from a DNA library, includes: adding a negative control sequence and a positive control sequence to the DNA library, or picking a negative control sequence and/or a positive control sequence from the library; determining a pre-capture amount of the negative control sequence and a pre-capture amount of the positive control sequence; performing enrichment of a target sequence from the DNA library using a bait sequence to produce a post-capture library; determining a post-capture amount of the negative control sequence and a post-capture amount of the positive control sequence in the post-capture library; and determining the efficiency of the target enrichment, based on the post-capture amount of the positive control sequence, the post-capture amount of the negative control sequence, the pre-capture amount of the positive control sequence, and the pre-capture amount of the negative control sequence.

    摘要翻译： 用于确定DNA文库的靶富集效率的方法包括：向DNA文库添加阴性对照序列和阳性对照序列，或从文库中挑选阴性对照序列和/或阳性对照序列; 确定阴性对照序列的预捕获量和阳性对照序列的预捕获量; 使用诱饵序列从DNA文库中进行目标序列的富集以产生捕获后文库; 确定后捕获库中的阴性对照序列的后捕获量和阳性对照序列的后捕获量; 基于阳性对照序列的后捕获量，阴性对照序列的后捕获量，阳性对照序列的预捕获量和预捕获量，确定目标浓缩的效率 的阴性对照序列。

    DNA Polymerases
    35.
    发明申请
    DNA Polymerases 有权
    DNA聚合酶

    公开(公告)号：US20130034879A1

    公开(公告)日：2013-02-07

    申请号：US13564940

    申请日：2012-08-02

    申请人： Remigijus Skirgaila ,  Agne Tubeleviciute ,  Renata Rimseliéné ,  Sigitas Burinskas

    发明人： Remigijus Skirgaila ,  Agne Tubeleviciute ,  Renata Rimseliéné ,  Sigitas Burinskas

    IPC分类号： C12N9/12 ,  C40B10/00 ,  C12P19/34

    CPC分类号： C12N9/1252 ,  C07K2319/21 ,  C12Y207/07007

    摘要： A DNA polymerase mutant comprising a Taq DNA polymerase amino acid sequence with a mutation at one or more of the following selected amino acid positions: E189K, E230K, E507K, H28R, L30R, G38R, F73V, H75R, E76A, E76G, E76K, E90K, K206R, E315K, A348V, L351F, A439T, D452N, G504S, E507A, D551N, L552R, I553V, D578N, H676R, Q680R, D732G, E734G, E734K, F749V; wherein the polymerase mutant exhibits relative to wild-type DNA polymerase increased polymerase speed, increased affinity to DNA substrate and/or increased resistance to a DNA polymerase inhibitor; and wherein, when the mutation is E507K in combination with two or more further mutations or the mutation is Q680R in combination with four or more further mutations, at least one of the further mutations is at one of the selected amino acid positions; and when the mutation is I553V, this is not in combination with D551S.

    摘要翻译： 一种DNA聚合酶突变体，其包含在一个或多个以下选择的氨基酸位置具有突变的Taq DNA聚合酶氨基酸序列：E189K，E230K，E507K，H28R，L30R，G38R，F73V，H75R，E76A，E76G，E76K，E90K ，K206R，E315K，A348V，L351F，A439T，D452N，G504S，E507A，D551N，L552R，I553V，D578N，H676R，Q680R，D732G，E734G，E734K，F749V; 其中所述聚合酶突变体相对于野生型DNA聚合酶显示出增加的聚合酶速度，对DNA底物的增加的亲和力和/或对DNA聚合酶抑制剂的增加的抗性; 并且其中当所述突变与两个或更多个其它突变组合时为E507K时，或者突变为Q680R与四个或更多个其它突变组合时，至少一个其它突变位于选定氨基酸位置之一; 当突变为I553V时，这不与D551S组合。

    Selection and Enrichment of Proteins Using In Vitro Compartmentalization
    36.
    发明申请
    Selection and Enrichment of Proteins Using In Vitro Compartmentalization 有权
    蛋白质的选择和富集使用体外分区

    公开(公告)号：US20120172236A1

    公开(公告)日：2012-07-05

    申请号：US13415504

    申请日：2012-03-08

    申请人： Yu Zheng ,  Richard J. Roberts

    发明人： Yu Zheng ,  Richard J. Roberts

    IPC分类号： C40B10/00 ,  C40B40/08

    CPC分类号： C12N15/1058 ,  C12N9/22 ,  C12N15/1075

    摘要： Compositions and methods are provided for selection and enrichment of a target gene from a library of polynucleotide sequences such as might be formed from a genome or by random mutagenesis of a genetic sequence. The selection and enrichment occurs in aqueous droplets formed in an emulsion that compartmentalize individual polynucleotides from the library or a plurality of polynucleotides that may include polynucleotides not derived from the library, transcription and translation reagents and optionally additional chemical and enzyme reagents. The selection and enrichment method utilizes a polynucleotide adaptor which when ligated to the polynucleotide fragment enables amplification to occur in the presence of an adaptor specific primer.

    摘要翻译： 提供了组合物和方法用于从多基因序列文库中选择和富集靶基因，所述多核苷酸序列可能由基因组形成，或通过遗传序列的随机诱变。 选择和富集发生在形成于乳剂中的水性液滴中，其从文库分离单个多核苷酸或多个多核苷酸，其可以包括不衍生自文库，转录和翻译试剂以及任选的其它化学和酶试剂的多核苷酸。 选择和富集方法利用多核苷酸衔接子，当连接到多核苷酸片段时，可以在衔接子特异性引物的存在下进行扩增。

    SURFACE DISPLAY OF RECOMBINANT PROTEINS IN LOWER EUKARYOTES
    39.
    发明申请
    SURFACE DISPLAY OF RECOMBINANT PROTEINS IN LOWER EUKARYOTES 有权
    重组蛋白的表面显示在较低的毒素中

    公开(公告)号：US20100331192A1

    公开(公告)日：2010-12-30

    申请号：US12863911

    申请日：2009-02-20

    申请人： Dongxing Zha ,  Stefan Wildt

    发明人： Dongxing Zha ,  Stefan Wildt

    IPC分类号： C40B10/00 ,  G01N33/53

    CPC分类号： C12N15/1037 ,  C07K16/005 ,  C07K16/2887 ,  C07K16/32 ,  C07K16/40 ,  C07K2317/51 ,  C07K2317/55 ,  C12N15/1055 ,  C40B40/02 ,  C40B40/08 ,  C40B50/06

    摘要： Methods for display of recombinant proteins or protein libraries on the surface of lower eukaryotes such as yeast and filamentous fungi are described. The methods are useful for screening libraries of recombinant proteins in lower eukaryotes to identify particular proteins with desired properties from the array of proteins in the libraries. The methods are particularly useful for constructing and screening antibody libraries in lower eukaryotes.

    摘要翻译： 描述了在低等真核生物如酵母和丝状真菌的表面上显示重组蛋白或蛋白质文库的方法。 该方法可用于筛选低等真核生物中的重组蛋白的文库，以从文库中的蛋白质阵列鉴定具有所需性质的特定蛋白质。 该方法对于构建和筛选低等真核生物中的抗体文库特别有用。

    Synthetic Binding Proteins
    40.
    发明申请
    Synthetic Binding Proteins 有权
    合成结合蛋白

    公开(公告)号：US20100304462A1

    公开(公告)日：2010-12-02

    申请号：US12849673

    申请日：2010-08-03

    申请人： Richard D. Morgan

    发明人： Richard D. Morgan

    IPC分类号： C12N9/16 ,  C40B50/02 ,  C40B10/00 ,  C40B60/14

    CPC分类号： C12N9/22 ,  C12N15/1089 ,  G06F19/18 ,  G06F19/22

    摘要： Methods and compositions are provided for creating a binding protein that recognizes a rationally chosen recognition sequence in which a first amino acid has been substituted for a second amino acid using site-directed mutagenesis of a member protein of a set of proteins at an identified position or positions correlated with recognition of a chosen specified target module in the recognition sequence. A system is provided for automating the storage and manipulation of the correlations between positions and types of amino acid residues in the binding protein with specific modules at specified positions in the target recognition sequence and for designing and creating proteins with novel specificities.

    摘要翻译： 提供了方法和组合物，用于产生结合蛋白，其识别合理选择的识别序列，其中第一氨基酸已被取代第二个氨基酸，使用在鉴定位置的一组蛋白质的成员蛋白质的定点突变，或 与识别序列中所选择的指定目标模块的识别相关的位置。 提供了一种系统，用于使目标识别序列中的特定位置处的特定模块的结合蛋白中的氨基酸残基的位置和类型之间的相关性的存储和操作自动化并且用于设计和产生具有新特异性的蛋白质。