公开(公告)号：US20150133649A1

公开(公告)日：2015-05-14

申请号：US14536429

申请日：2014-11-07

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Brian REED ,  James A. Ball

IPC: B01D17/04 ,  C07H21/00 ,  C07H1/06

CPC classification number: B01D17/047 ,  B01D17/04 ,  B01D17/041 ,  C07H1/06 ,  C07H21/00 ,  C12Q1/6806 ,  C12Q2527/125 ,  C12Q2527/137 ,  C12Q2563/159

Abstract: A method of recovering a bead support from an emulsion includes supplying an aqueous surfactant solution into a centrifuge tube; supplying a hydrophobic liquid over the surfactant solution in the centrifuge tube, wherein a ratio of the volume of the aqueous surfactant solution to the volume of the hydrophobic liquid is not greater than 0.5; and applying an emulsion over the hydrophobic liquid while centrifuging, the emulsion comprising a dispersed aqueous phase including the bead support, the emulsion breaking and material of the dispersed phase preferentially partitioning to the surfactant solution.

Abstract translation: 从乳液中回收珠粒载体的方法包括将表面活性剂水溶液供给到离心管中; 在离心管中的表面活性剂溶液上提供疏水性液体，其中表面活性剂水溶液的体积与疏水性液体的体积之比不大于0.5; 并且在离心时将乳液施加在疏水性液体上，所述乳液包含分散的水相，包括珠粒支持体，乳液破碎和分散相的材料优先分配至表面活性剂溶液。

公开(公告)号：US09024002B2

公开(公告)日：2015-05-05

申请号：US13788715

申请日：2013-03-07

Applicant: Life Technologies Corporation

Inventor： Craig Cummings ,  Olga Petrauskene ,  Lily Wong ,  Allison De Los Reyes

IPC: C12Q1/68 ,  C12P19/34

CPC classification number: C12Q1/689 ,  C12Q2600/158 ,  C12Q2600/16 ,  Y02A50/451

Abstract: Described are compositions, methods and kits for detection and/or differential detection of serovars of Salmonella enterica subsp. enterica serovar such as S. Heidelberg and S. Hadar in samples. Some embodiments relate to multiplex amplification based molecular assays.

Abstract translation: 描述了用于检测和/或差异检测肠炎沙门氏菌血清型的组合物，方法和试剂盒。 肠溶血清病例如海德堡和S.Hadar在样品中。 一些实施方案涉及基于多重扩增的分子测定。

公开(公告)号：US20150104823A1

公开(公告)日：2015-04-16

申请号：US14521346

申请日：2014-10-22

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Daniel BEACHAM ,  Kyle GEE

IPC: G01N33/84 ,  G01N33/68 ,  C12Q1/02

CPC classification number: G01N33/84 ,  C12Q1/02 ,  G01N33/6872 ,  G01N2333/705

Abstract: The present invention relates to methods for detecting the activity of an ion channel in a cell. The methods comprise providing a loading buffer solution to a cell that has an ion channel. The loading buffer comprises at least one thallium indicator (e.g., an environmentally sensitive, luminescent dye) and a physiological concentration of chloride ions. The methods further comprise providing a stimulus buffer to the cell, wherein the stimulus buffer comprises thallium (e.g., thallium ions). Providing the stimulus buffer causes thallium influx into the cell through the ion channel. After providing the stimulus buffer, the luminescence (e.g., fluorescence) of the dye in the cell is detected. The luminescence of the dye can change in the presence or absence of thallium. The methods may be used to measure influx or efflux of thallium through an ion channel.

Abstract translation: 本发明涉及用于检测细胞中离子通道的活性的方法。 所述方法包括向具有离子通道的电池提供加载缓冲溶液。 加载缓冲液包含至少一种铊指示剂（例如，环境敏感的发光染料）和氯离子的生理浓度。 所述方法还包括向细胞提供刺激缓冲液，其中刺激缓冲液包含铊（例如铊离子）。 提供刺激缓冲液使铊通过离子通道流入细胞。 在提供刺激缓冲液之后，检测细胞中染料的发光（例如荧光）。 在存在或不存在铊的情况下，染料的发光可以改变。 该方法可用于测量铊通过离子通道的流入或流出。

公开(公告)号：US09005427B2

公开(公告)日：2015-04-14

申请号：US14151270

申请日：2014-01-09

Applicant: Life Technologies Corporation

Inventor： Jonathan Rothberg ,  Wolfgang Hinz

IPC: G01N27/414 ,  G01N33/50 ,  G01N33/543 ,  C12Q1/68 ,  H01L29/78 ,  G01N27/26 ,  G01N33/68

CPC classification number: C12Q1/6825 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N27/26 ,  G01N27/4145 ,  G01N27/4148 ,  G01N33/54373 ,  G01N33/6818 ,  H01L29/78 ,  H01L29/788 ,  H01L2924/01006

Abstract: Methods and apparatuses relating to large scale FET arrays for analyte detection and measurement are provided. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes.

公开(公告)号：US20150097610A1

公开(公告)日：2015-04-09

申请号：US14569289

申请日：2014-12-12

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Keith G. FIFE ,  Jungwook Yang

IPC: G01N27/414 ,  H03K5/003

CPC classification number: G01N27/4148 ,  H03K5/003 ,  Y10S436/806 ,  Y10S436/807 ,  Y10T436/11

Abstract: An array of sensors arranged in matched pairs of transistors with an output formed on a first transistor and a sensor formed on the second transistor of the matched pair. The matched pairs are arranged such that the second transistor in the matched pair is read through the output of the first transistor in the matched pair. The first transistor in the matched pair is forced into the saturation (active) region to prevent interference from the second transistor on the output of the first transistor. A sample is taken of the output. The first transistor is then placed into the linear region allowing the sensor formed on the second transistor to be read through the output of the first transistor. A sample is taken from the output of the sensor reading of the second transistor. A difference is formed of the two samples.

Abstract translation: 布置成匹配的晶体管对的传感器阵列，其具有形成在第一晶体管上的输出和形成在匹配对的第二晶体管上的传感器。 匹配对被布置成使得匹配对中的第二晶体管通过匹配对中的第一晶体管的输出读取。 匹配对中的第一晶体管被迫进入饱和（有源）区域，以防止第一晶体管的输出上的第二晶体管的干扰。 取出输出的样本。 然后将第一晶体管放置在线性区域中，允许通过第一晶体管的输出读取形成在第二晶体管上的传感器。 样品从第二晶体管的传感器读数的输出中取出。 两个样本形成差异。

公开(公告)号：US20150090936A1

公开(公告)日：2015-04-02

申请号：US14566166

申请日：2014-12-10

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Yi-Zhen HU ,  Hee Chol KANG

IPC: C09K11/06 ,  C07D209/26 ,  C07H19/207 ,  C07D401/10

CPC classification number: C07H19/207 ,  C07D209/14 ,  C07D209/26 ,  C07D401/10 ,  C07D401/14 ,  C09B23/0041 ,  C09B23/0066 ,  C09B23/086 ,  C09B23/164 ,  C09K11/06 ,  C09K2211/1007 ,  C09K2211/1014 ,  C09K2211/1022 ,  C09K2211/1044 ,  C09K2211/1059 ,  C09K2211/1074 ,  G01N33/52

Abstract: Provided herein are heptamethine cyanine dyes having a large Stokes shift, and the salts and conjugates thereof. Also provided are methods of using and making such large Stokes shift dyes as fluorescence resonance energy transfer (FRET) acceptors or donors.

Abstract translation: 本文提供具有大的斯托克斯位移的七甲基氰花青染料及其盐和共轭物。 还提供了使用和制备这种大的斯托克斯位移染料作为荧光共振能量转移（FRET）受体或供体的方法。

公开(公告)号：US20150087530A1

公开(公告)日：2015-03-26

申请号：US14498979

申请日：2014-09-26

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jon A. HOSHIZAKI ,  Joon Mo YANG ,  Maryam SHARIATI ,  David M. COX ,  Kirk M. HIRANO ,  John BRIDGHAM ,  George Stefan GOLDA ,  Sam Lee WOO

IPC: C12Q1/68

CPC classification number: C12Q1/6869 ,  B01F13/0059 ,  B01F13/0222 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00466 ,  B01J2219/00527 ,  B01J2219/00576 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01L3/502707 ,  B01L3/50273 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0684 ,  B01L2200/16 ,  B01L2300/0819 ,  B01L2300/0822 ,  B01L2300/0867 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2300/1822 ,  B01L2400/0427 ,  C12Q1/6874 ,  G01N35/00069 ,  G01N35/1002 ,  G01N35/1097 ,  G01N2035/00148 ,  G01N2035/00237 ,  G01N2035/1034 ,  G02B21/34 ,  Y10T436/11 ,  Y10T436/143333

Abstract: Various embodiments of a low-volume sequencing system are provided herein. The system can include a low-volume flowcell having at least one reaction chamber of a defined volume (e.g., less than about 100 μl). The system can also include an automated reagent delivery mechanism configured to reversibly couple with the inlet port corresponding to a target reaction chamber thereby placing allowing for reagent to be accurately moved from a storage container to the reaction chamber with minimal reagent waste. The flowcells can include a plurality of reaction chambers (e.g., 6) thereby allowing for parallel analysis of multiple samples. Various methods of analyzing a biomolecule are also provided herein.

Abstract translation: 本文提供了低体积测序系统的各种实施方案。 该系统可以包括具有至少一个具有确定体积（例如小于约100μl）的反应室的低体积流动池。 该系统还可以包括配置成与对应于目标反应室的入口端可逆耦合的自动化试剂输送机构，从而使试剂能够以最小的试剂废物从试剂容器准确地移动到反应室。 流通池可以包括多个反应室（例如6），从而允许多个样品的并行分析。 本文还提供了分析生物分子的各种方法。

公开(公告)号：US20150083232A1

公开(公告)日：2015-03-26

申请号：US14558321

申请日：2014-12-02

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： David M. COX

IPC: B01L9/00 ,  F16M13/02

CPC classification number: B01L9/50 ,  B01L3/502 ,  B01L3/502715 ,  B01L9/52 ,  B01L9/527 ,  B01L2200/025 ,  B01L2200/026 ,  B01L2200/12 ,  B01L2300/0609 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0816 ,  F16M13/02 ,  Y10S439/912 ,  Y10T137/0402 ,  Y10T137/598 ,  Y10T137/6966

Abstract: A vertical clamping device is provided that supports a flow cell component in a vertical configuration in which the flow cell is on an opposite side of the vertical support from the electronic interface. The clamp includes a vertical setting to receive the flow cell component and provides an electronic interface on a vertical surface of the vertical setting. A block supports the fluidics interface and can move in a horizontal direction bringing the fluidics interface into contact with the flow cell component.

公开(公告)号：US20150064829A1

公开(公告)日：2015-03-05

申请号：US14543551

申请日：2014-11-17

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Keith G. FIFE ,  James BUSTILLO ,  Jordan OWENS

IPC: G01N27/414

CPC classification number: G01N27/414 ,  G01N27/4145

Abstract: In one implementation, a chemical sensor is described. The chemical sensor includes chemically-sensitive field effect transistor including a floating gate conductor having an upper surface. A dielectric material defines an opening extending to the upper surface of the floating gate conductor. A conductive sidewall spacer is on a sidewall of the opening and contacts the upper surface of the floating gate conductor.

Abstract translation: 在一个实施方式中，描述了化学传感器。 化学传感器包括具有上表面的浮栅导体的化学敏感场效应晶体管。 介电材料限定了延伸到浮动栅极导体的上表面的开口。 导电侧壁间隔物位于开口的侧壁上并与浮栅导体的上表面接触。

公开(公告)号：US20150064785A1

公开(公告)日：2015-03-05

申请号：US14367413

申请日：2012-12-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Richard Fike ,  Shawn Barrett ,  Zhou Jiang ,  David Judd

IPC: C12N5/00 ,  B29C67/24

CPC classification number: C12N5/005 ,  B01J13/06 ,  B01J13/22 ,  B29C67/24 ,  B29L2009/00 ,  C12N5/0018 ,  C12N2500/30 ,  C12N2500/38 ,  C12N2511/00 ,  C12N2533/40 ,  C12N2533/74

Abstract: Compositions and methods are described for preparing media, feeds, and supplements. Such methods and medias may display increased stability of labile components and may use, for example, microsuspension and/or encapsulation technologies, chelation, and optionally, coating and/or mixing the labile compounds with anti-oxidants. The compositions may withstand thermal and/or irradiation treatment and have reduced virus number. These techniques may result in product with extended shelf-life, extended release of their internal components into culture, or in product that can be added aseptically into a bioreactor using minimal volumes. The compositions and methods may optimize the bioproduction workflow and increase efficiency.

Abstract translation: 描述了制备培养基，饲料和补品的组合物和方法。 这样的方法和介质可以显示不稳定组分的增加的稳定性，并且可以使用例如微悬浮和/或封装技术，螯合，以及任选地，将不稳定化合物与抗氧化剂包衣和/或混合。 组合物可以承受热和/或照射治疗并且具有减少的病毒数量。 这些技术可能导致产品具有延长的保质期，将其内部组分延长释放到培养物中，或可以无菌地添加到生物反应器中使用最小体积的产品中。 组合物和方法可以优化生物生产工作流程并提高效率。