Abstract:
An apparatus and method for electrochemically modifying the retention of a species on a chromatography material is disclosed. The apparatus comprises a housing having an effluent flow channel adapted to permit fluid flow therethrough. The effluent flow channel comprises chromatography material. The apparatus further comprises first and second electrodes positioned such that at least a portion of the chromatography material is disposed between the first and second electrodes, and fluid flow through the apparatus is between, and in contact with, the first and second electrodes.
Abstract:
A controlled charge chromatography column for the purification of a fluid-containing material, which column comprises a chromatographic column having an inlet for the introduction of a fluid to be purified and an outlet for the discharge of the purified fluid, and one or more concentrated materials and a flow-through capacitor disposed within the column between the inlet and the outlet, the flow-through capacitor means comprising a plurality of spirally wound or stacked washer layers to include a first electrically conductive backing layer, such as of graphite, and a first high surface area conductive layer secured to the backing layer, such as composed of porous carbon fibers and a non-conductive, porous spacer layer to electrically insulate the backing and conductive layer and to permit the flow of material therethrough, the flow-through capacitor to be connected to a DC power source to charge the respective conductive layers with different polarities whereby a fluid containing material through the column is purified by the electrically conductive stationary phase and the retention thereof onto the high surface area layer and permitting for example the purification of solutions of liquids, such as salt, and providing for the recovery of a purified liquid.
Abstract:
A controlled charge chromatography column for the purification of a fluid containing materials, which column comprises a chromatographic column having an inlet for the introduction of a fluid to be purified and an outlet for the discharge of the purified fluid, and one or more concentrated materials and a flow-through capacitor disposed within the column between the inlet and the outlet, the flow-through capacitor means comprising a plurality of spirally wound, spaced apart layers to include a first electrically conductive backing layer, such as of graphite, and a first high surface area conductive layer secured to the backing layer, such as composed of porous carbon fibers and a non-conductive, porous spacer layer to electrically insulate the backing and conductive layer and to permit the flow of material therethrough, the flow-through capacitor to be connected to a DC power source to charge the respective conductive layers with different polarities whereby a fluid containing material through the column is purified by the electrically conductive stationary phase and the retention thereof onto the high surface area layer and permitting for example the purification of solutions of liquids, such as salt, and providing for the recovery of a purified liquid.
Abstract:
A controlled charge chromatography column for the purification of a fluid containing materials, which column comprises a chromatographic column having an inlet for the introduction of a fluid to be purified and an outlet for the discharge of the purified fluid, and one or more concentrated materials and a flow-through capacitor disposed within the column between the inlet and the outlet, the flow-through capacitor means comprising a plurality of spirally wound, spaced apart layers to include a first electrically conductive backing layer, such as of graphite, and a first high surface area conductive layer secured to the backing layer, such as composed of porous carbon fibers and a non-conductive, porous spacer layer to electrically insulate the backing and conductive layer and to permit the flow of material therethrough, the flow-through capacitor to be connected to a DC power source to charge the respective conductive layers with different polarities whereby a fluid containing material through the column is purified by the electrically conductive stationary phase and the retention thereof onto the high surface area layer and permitting for example the purification of solutions of liquids, such as salt, and providing for the recovery of a purified liquid.
Abstract:
An electrokinetic method for separating molecular species employs the surface of moving charged colloidal particles in a buffered dispersing medium, in a process which combines electrophoresis and a variant of chromatography in which the "stationary phase" surface moves. The colloidal particles have on their surface a material having the ability to interact selectively with the molecular species to be separated. The surface of the particles as originally formed may have such an ability, or the surface is modified to produce it. In one embodiment the colloidal particles are negatively charged and the surface-modifying materials are metal ions. In another embodiment the surface of the colloidal particles is modified by the incorporation of affinity groups. The analytes distribute themselves between the dispersing medium and the surface of the colloidal particles, resulting in analyte migration rates which are functions of the individual distribution equilibrium constants and the rate of colloidal particle migration. A capillary column for conducting the method includes surface-modified charged colloidal particles in a buffered dispersing medium.
Abstract:
THE SOLUTES OF A SAMPLE TO BE SEPARATED CHROMATOGRAPHICALLY ARE FIRST PLATED ONTO AN ELECTRODE. THIS ELECTRODE IS THEN MAINTAINED IN THE INLET OF A CHROMATOGRAPHIC SYSTEM WHERE BY REVERSAL OF THE CURRENT THE SAMPLE IS RETURNED TO SOLUTION. BY CONTROLLING THE SIZE OF THE ELECTRODE AND THE TIME TAKEN FOR THE SAMPLE TO RETURN TO SOLUTION, THE PLATE HEIGHT CONTRIBUTION OF THE INLET IS LIMITED. A PRELIMINARY PARTIAL SEPARATION OF THE SAMPLE BY ELECTROLYTIC MEANS IS ALSO PROVIDED FOR.
Abstract:
The invention relates to a system, comprising: a) a sample processing unit, comprising an input port and an output port coupled to a rotating container having at least one sample chamber, the sample processing unit configured provide a first processing step to a sample or to rotate the container so as to apply a centrifugal force to a sample deposited in the chamber and separate at least a first component and a second component of the deposited sample; and b) a sample separation unit coupled to the output port of the sample processing unit, the cell separation unit comprising separation column holder (42), a pump (64) and a plurality of valves (1-11) configured to at least partially control fluid flow through a fluid circuitry and a separation column (40) positioned in the holder, the separation column configured to separate labeled and unlabeled components of sample flowed through the column.
Abstract:
The present disclosure relates to a process, a system and a use for removing micropollutants (1) in liquid (2). The process comprises providing liquid (2) to a container (3) adapted to hold a liquid and/or a gas, providing magnetic activated carbon (4), mixing it, separating the magnetic activated carbon (4) using a magnetic separator (5), removing between 1 and 100% of the separated used magnetic activated carbon (4), removing the liquid (2), providing new liquid (2) to the container (3), providing the used magnetic activated carbon (4) to the container (3), adding between 1 and 100% of unused magnetic activated carbon (4), repeating the mixing and separation steps at least one time. The process allows for control of several parameters, such as the flow rate of the liquid, dosage of MAC and ratio used/unused MAC required to remove micropollutants from the liquid.
Abstract:
The present invention related to an apparatus for separating micro-nano scale particles based on microfluidic chromatography using surface acoustic waves, comprising: a piezoelectric substrate; a pair of transducers, which are patterned on the piezoelectric substrate and generate surface acoustic waves when electric energy is applied to the piezoelectric substrate; a microfluidic chip, which is mounted on the piezoelectric substrate and include a microfluidic channel disposed between the pair of transducers, wherein a fluid including micro-nano scale particles flows in the microfluidic channel; and a detection unit, which detects micro-nano scale particles separated by the surface acoustic waves while the micro-nano scale particles pass through the microfluidic channel, wherein forces of the surface acoustic waves generated by the pair of transducers are formed in a direction opposite to a fluid flow to generate flow resistance to the micro-nano scale particles which flows in the microfluidic channel.
Abstract:
A method of applying microwave radiation to a chromatography column to achieve turbulent chromatography, increase the speed of column equilibration following a change of mobile phase, facilitate faster mixing of fluids, reduce the viscosity of fluids, and apply temperature pulses to selected time segments of a separation. The method may be used in conjunction with the use of columns packed with fused-core particles or monolithic columns. Improved size-based separations may be achieved using diffusion-and-turbulent-dependent size exclusion chromatography.