公开(公告)号：US20190316119A1

公开(公告)日：2019-10-17

申请号：US16400858

申请日：2019-05-01

Applicant: Raindance Technologies, Inc.

Inventor： Michael L. Samuels ,  Jeffrey Charles Olson ,  Andrew Watson ,  Keith Brown ,  Darren R. Link

IPC: C12N15/10 ,  C40B50/08 ,  C12Q1/6874 ,  G01N33/543 ,  G01N33/58

Abstract: The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.

公开(公告)号：US20180223348A1

公开(公告)日：2018-08-09

申请号：US15947346

申请日：2018-04-06

Applicant: Raindance Technologies, Inc.

Inventor： Darren R. Link ,  Michael L. Samuels

IPC: C12Q1/6844 ,  C12Q1/6858

CPC classification number: C12Q1/6844 ,  C12Q1/6858 ,  C12Q2535/122 ,  C12Q2563/159 ,  C12Q2563/179 ,  C12Q2565/629

Abstract: The invention generally relates to methods for distinguishing a rare genetic variation in a nucleic acid sequence.

公开(公告)号：US09745617B2

公开(公告)日：2017-08-29

申请号：US15019355

申请日：2016-02-09

Applicant: Raindance Technologies, Inc.

Inventor： Jonathan William Larson ,  Qun Zhong ,  Darren R. Link

IPC: C12Q1/68

CPC classification number: C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/686 ,  C12Q1/6883 ,  C12Q1/6886 ,  C12Q2600/172 ,  C12Q2563/159 ,  C12Q2565/629 ,  C12Q2537/143 ,  C12Q2531/113 ,  C12Q2535/131

Abstract: The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.

公开(公告)号：US20160201125A1

公开(公告)日：2016-07-14

申请号：US14995744

申请日：2016-01-14

Applicant: Raindance Technologies, Inc.

Inventor： Michael L. Samuels ,  Jeffrey Charles Olson ,  Andrew Watson ,  Keith Brown ,  Darren R. Link

IPC: C12Q1/68

Abstract: The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.

Abstract translation: 本发明提供条形码文件库及其制备和使用方法，包括获得多个核酸构建体，其中每个构建体包含独特的N-聚体和功能性N-聚体，并将构建体分离成流体隔室，使得每个隔室含有一个 或更多的独特构造的副本。 本发明还提供数字PCR和数字PCR中条形码库的使用方法。

公开(公告)号：US09127310B2

公开(公告)日：2015-09-08

申请号：US14264686

申请日：2014-04-29

Applicant: Raindance Technologies, Inc.

Inventor： Jonathan William Larson ,  Qun Zhong ,  Darren R. Link

IPC: C12P19/34 ,  C12Q1/68

CPC classification number: C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/686 ,  C12Q1/6883 ,  C12Q1/6886 ,  C12Q2600/172 ,  C12Q2563/159 ,  C12Q2565/629 ,  C12Q2537/143 ,  C12Q2531/113 ,  C12Q2535/131

Abstract: The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.

Abstract translation: 本发明一般涉及基于液滴的数字PCR和使用其进行目标核酸分析的方法。 在某些实施方案中，本发明的方法包括形成含有平均单个靶核酸的样品液滴，扩增液滴中的靶，不包含来自靶标的扩增子的液滴和来自靶标变体的扩增子，并分析靶扩增子 。

公开(公告)号：US20150099266A1

公开(公告)日：2015-04-09

申请号：US14505974

申请日：2014-10-03

Applicant: Raindance Technologies, Inc.

Inventor： Michael Samuels ,  Jeffrey Olson ,  Darren R. Link

IPC: G06F19/18 ,  G06F19/00 ,  C12Q1/68

CPC classification number: G16B20/00

Abstract: In certain aspects, methods of the invention involve performing modification state specific enzymatic reaction of nucleic acid in a sample, determining a value associated with efficiency of the modification state specific enzymatic reaction based on a control, determining an amount of target nucleic acid in the sample, and normalizing the amount of target nucleic acid based on the efficiency value. Based on the normalized amount of target nucleic acid, the method further includes determining whether the normalized amount of target nucleic acid is indicative of a condition.

Abstract translation: 在某些方面，本发明的方法涉及在样品中进行核酸的修饰状态特异性酶反应，基于对照确定与修饰状态特异性酶促反应的效率相关的值，确定样品中靶核酸的量 ，并根据效率值使目标核酸的量归一化。 基于目标核酸的归一化量，该方法还包括确定目标核酸的归一化量是否指示条件。

公开(公告)号：US20140256595A1

公开(公告)日：2014-09-11

申请号：US14226482

申请日：2014-03-26

Applicant: RAINDANCE TECHNOLOGIES, INC.

Inventor： Darren R. Link ,  Laurent Boitard ,  Jeffrey Branciforte ,  Yves Charles ,  Gilbert Feke ,  John Q. Lu ,  David Marran ,  Ahmadali Tabatabai ,  Michael Weiner ,  Wolfgang Hinz ,  Jonathan M. Rothberg

IPC: C12Q1/68

CPC classification number: C12N15/1075 ,  B01F3/0807 ,  B01F13/0071 ,  B01F13/0076 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00459 ,  B01J2219/00466 ,  B01J2219/00468 ,  B01J2219/00479 ,  B01J2219/005 ,  B01J2219/00576 ,  B01J2219/00599 ,  B01J2219/00702 ,  B01J2219/0072 ,  B01J2219/00743 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502746 ,  B01L3/502761 ,  B01L3/502784 ,  B01L3/502792 ,  B01L3/565 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/0621 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0896 ,  B01L2400/0415 ,  B01L2400/0424 ,  B01L2400/0487 ,  B01L2400/084 ,  B82Y5/00 ,  B82Y30/00 ,  C07K1/047 ,  C12Q1/6804 ,  C12Q1/6816 ,  C12Q1/6825 ,  C12Q1/6834 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q2525/161 ,  C12Q2561/119 ,  C12Q2563/103 ,  C12Q2563/107 ,  C12Q2563/159 ,  C12Q2565/537 ,  C12Q2565/629 ,  C12Q2600/16 ,  C40B30/04 ,  C40B40/04 ,  C40B50/08 ,  C40B60/08 ,  C40B60/12 ,  C40B70/00 ,  G01N21/6428 ,  G01N21/6445 ,  G01N21/6458 ,  G01N33/536 ,  G01N33/542 ,  G01N33/543 ,  G01N33/6845 ,  G01N33/6854 ,  G01N2500/10

Abstract: The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. The invention provides for aqueous based emulsions containing uniquely labeled cells, enzymes, nucleic acids, etc., wherein the emulsions further comprise primers, labels, probes, and other reactants. An oil based carrier-fluid envelopes the emulsion library on a microfluidic device, such that a continuous channel provides for flow of the immiscible fluids, to accomplish pooling, coalescing, mixing, sorting, detection, etc., of the emulsion library.

公开(公告)号：US09981230B2

公开(公告)日：2018-05-29

申请号：US13779943

申请日：2013-02-28

Applicant: RainDance Technologies, Inc.

Inventor： Darren R. Link ,  Michael Weiner ,  David Marran ,  Jonathan M. Rothberg

IPC: C12Q1/68 ,  C12P19/34 ,  B01F3/08 ,  B01F13/00 ,  B01J19/00 ,  B01L3/00 ,  B03C5/00 ,  B03C5/02 ,  C12Q1/00 ,  G01N15/14 ,  G01N21/05 ,  G01N21/64 ,  G01N35/08 ,  G01N27/327 ,  C12N15/10 ,  B01L7/00 ,  B01L9/00 ,  G01N35/00 ,  G01N21/03

CPC classification number: B01F3/0807 ,  B01F13/0062 ,  B01J19/0093 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/502784 ,  B01L3/565 ,  B01L7/525 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/165 ,  B01L2400/0415 ,  B01L2400/0424 ,  B01L2400/0487 ,  B01L2400/086 ,  B03C5/005 ,  B03C5/026 ,  C12N15/1068 ,  C12N15/1086 ,  C12Q1/00 ,  C12Q1/6806 ,  C12Q1/6837 ,  C12Q1/6844 ,  C12Q1/6855 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2565/628 ,  C12Q2565/629 ,  G01N15/147 ,  G01N21/05 ,  G01N21/64 ,  G01N27/3275 ,  G01N35/08 ,  G01N2021/0346 ,  G01N2035/00237 ,  G01N2035/00326 ,  G01N2201/024 ,  Y10T137/87571 ,  Y10T137/87619 ,  Y10T137/87652 ,  C12Q2525/191 ,  C12Q2563/159 ,  C12Q2535/101 ,  C12Q2563/149 ,  C12Q2531/113

Abstract: The present invention provides novel microfluidic substrates and methods that are useful for performing biological, chemical and diagnostic assays. The substrates can include a plurality of electrically addressable, channel bearing fluidic modules integrally arranged such that a continuous channel is provided for flow of immiscible fluids.

公开(公告)号：US09938572B1

公开(公告)日：2018-04-10

申请号：US15259798

申请日：2016-09-08

Applicant: RainDance Technologies, Inc.

Inventor： Darren R. Link ,  Patrick Odoy ,  Akim Lennhoff ,  John Luckey ,  Xinyu Li

IPC: C12Q1/68 ,  B01J13/00 ,  C07H21/04

CPC classification number: C12Q1/686 ,  B01J13/00 ,  C12Q2563/159

Abstract: Systems and methods are provided for forming emulsions from an aqueous fluid and oil. The aqueous fluid-in-oil emulsions are useful for numerous molecular biology techniques. The system is configured to cyclically translate a chamber containing the emulsion components, along a cycle path, while maintaining the axial orientation of the chamber with respect to a horizontal plane, and without rotating the chamber.

公开(公告)号：US09562837B2

公开(公告)日：2017-02-07

申请号：US13855318

申请日：2013-04-02

Applicant: Raindance Technologies, Inc.

Inventor： Darren R. Link

IPC: B01L3/00 ,  G01N1/38 ,  C12Q1/68 ,  B01J19/00 ,  B01F13/00 ,  B03C5/00 ,  B03C5/02 ,  B01F3/08 ,  C12Q1/00 ,  G01N35/08 ,  G01N15/14 ,  C12N15/10 ,  G01N1/10 ,  B01L7/00 ,  B01L9/00 ,  G01N35/00

CPC classification number: C12Q1/6848 ,  B01F3/0807 ,  B01F13/0062 ,  B01J19/0093 ,  B01J2219/00783 ,  B01J2219/00788 ,  B01J2219/00828 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00837 ,  B01J2219/00853 ,  B01J2219/0086 ,  B01J2219/00869 ,  B01J2219/00889 ,  B01J2219/00903 ,  B01J2219/0097 ,  B01J2219/00988 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/502753 ,  B01L3/502776 ,  B01L3/502784 ,  B01L3/565 ,  B01L7/525 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2200/141 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/165 ,  B01L2400/0415 ,  B01L2400/0424 ,  B01L2400/0487 ,  B01L2400/086 ,  B03C5/005 ,  B03C5/026 ,  B03C2201/26 ,  C12N15/1075 ,  C12Q1/00 ,  C12Q1/6806 ,  C12Q1/6827 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q1/6874 ,  C12Q2525/191 ,  C12Q2563/159 ,  C12Q2565/628 ,  C12Q2565/629 ,  G01N1/38 ,  G01N15/147 ,  G01N35/08 ,  G01N2035/00158 ,  G01N2035/00237 ,  G01N2035/00326 ,  C12Q2535/101 ,  C12Q2563/149

Abstract: The invention generally relates to assemblies for displacing droplets from a vessel that facilitate the collection and transfer of the droplets while minimizing sample loss. In certain aspects, the assembly includes at least one droplet formation module, in which the module is configured to form droplets surrounded by an immiscible fluid. The assembly also includes at least one chamber including an outlet, in which the chamber is configured to receive droplets and an immiscible fluid, and in which the outlet is configured to receive substantially only droplets. The assembly further includes a channel, configured such that the droplet formation module and the chamber are in fluid communication with each other via the channel. In other aspects, the assembly includes a plurality of hollow members, in which the hollow members are channels and in which the members are configured to interact with a vessel. The plurality of hollow members includes a first member configured to expel a fluid immiscible with droplets in the vessel and a second member configured to substantially only droplets from the vessel. The assembly also includes a main channel, in which the second member is in fluid communication with the main channel. The assembly also includes at least one analysis module connected to the main channel.

Abstract translation: 本发明一般涉及用于从容器移动液滴的组件，其有利于液滴的收集和转移同时最小化样品损失。 在某些方面，组件包括至少一个液滴形成模块，其中模块被配置成形成由不混溶流体包围的液滴。 组件还包括至少一个包括出口的室，其中室被构造成接收液滴和不混溶的流体，并且其中出口构造成仅基本上容纳液滴。 组件还包括通道，其构造成使得液滴形成模块和腔室经由通道彼此流体连通。 在其他方面，组件包括多个中空构件，其中中空构件是通道，构件中构件与容器相互作用。 多个中空构件包括构造成排出与容器中的液滴不混溶的流体的第一构件和构造成基本上仅从容器中滴下的第二构件。 组件还包括主通道，其中第二构件与主通道流体连通。 组件还包括连接到主通道的至少一个分析模块。