公开(公告)号：US06811980B2

公开(公告)日：2004-11-02

申请号：US09988978

申请日：2001-11-19

Applicant: William E. Ford ,  Jurina Wessels ,  Oliver Harnack

Inventor： William E. Ford ,  Jurina Wessels ,  Oliver Harnack

IPC: C12Q168

CPC classification number: C12Q1/6834 ,  B01J19/0046 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00635 ,  B01J2219/00722 ,  B01J2219/00729 ,  C07H21/00 ,  G01N33/54353 ,  Y10S977/924 ,  Y10T428/8305

Abstract: The present invention relates to a process for immobilization of nucleic acid molecules on a substrate, wherein the substrate is treated with atomic oxygen plasma prior to immobilizing the nucleic acid molecules thereon. The invention is further related to immobilized nucleic acid molecules and uses thereof.

Abstract translation: 本发明涉及将核酸分子固定在基质上的方法，其中在将核酸分子固定在其上之前，用原子氧等离子体处理基底。 本发明还涉及固定的核酸分子及其用途。

公开(公告)号：US06780582B1

公开(公告)日：2004-08-24

申请号：US09574692

申请日：2000-05-17

Applicant: Peter Wagner ,  Steffen Nock ,  Dana Ault-Riche ,  Christian Itin

Inventor： Peter Wagner ,  Steffen Nock ,  Dana Ault-Riche ,  Christian Itin

IPC: G01N33543

CPC classification number: B82Y5/00 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00619 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00635 ,  B01J2219/00637 ,  B01J2219/00644 ,  B01J2219/00659 ,  B01J2219/00702 ,  B01J2219/00725 ,  B82Y30/00 ,  C40B40/10 ,  G01N33/54393 ,  G01N33/551

Abstract: Arrays of protein-capture agents useful for the simultaneous detection of a plurality of proteins which are the expression products, or fragments thereof, of a cell or population of cells in an organism are provided. A variety of antibody arrays, in particular, are described. Methods of both making and using the arrays of protein-capture agents are also disclosed. The invention arrays are particularly useful for various proteomics applications including assessing patterns of protein expression and modification in cells.

Abstract translation: 提供了可用于同时检测生物体中细胞或细胞群的表达产物或其片段的多种蛋白质的蛋白质捕获剂的阵列。 特别描述了各种抗体阵列。 还公开了制备和使用蛋白质捕获剂的阵列的方法。 本发明阵列对于各种蛋白质组学应用特别有用，包​​括评估细胞中蛋白质表达和修饰的模式。

公开(公告)号：US06737026B1

公开(公告)日：2004-05-18

申请号：US09728209

申请日：2000-11-28

Applicant: H. Sam Bergh ,  Shenheng Guan

Inventor： H. Sam Bergh ,  Shenheng Guan

IPC: B01J1900

CPC classification number: B01L3/502715 ,  B01F5/0601 ,  B01F15/0264 ,  B01J19/0046 ,  B01J19/0093 ,  B01J2219/00286 ,  B01J2219/00306 ,  B01J2219/00308 ,  B01J2219/00313 ,  B01J2219/00317 ,  B01J2219/00337 ,  B01J2219/00351 ,  B01J2219/00353 ,  B01J2219/00389 ,  B01J2219/00418 ,  B01J2219/00452 ,  B01J2219/00479 ,  B01J2219/00495 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00635 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00657 ,  B01J2219/00659 ,  B01J2219/00704 ,  B01J2219/00707 ,  B01J2219/0072 ,  B01J2219/00745 ,  B01J2219/00747 ,  B01J2219/00759 ,  B01L3/5025 ,  B01L3/5027 ,  B01L3/502707 ,  B01L7/54 ,  B01L2200/0689 ,  B01L2200/146 ,  B01L2300/0864 ,  B01L2300/0874 ,  B01L2300/0887 ,  B01L2300/14 ,  B01L2300/18 ,  B01L2400/0487 ,  C01B3/40 ,  C07C17/23 ,  C07C45/34 ,  C07C45/35 ,  C07C45/38 ,  C07C45/46 ,  C07C45/54 ,  C07C45/72 ,  C40B30/08 ,  C40B40/18 ,  G01N30/90 ,  G01N31/10 ,  G01N35/028 ,  G01N2035/00158 ,  Y10T137/87885 ,  Y10T436/117497 ,  Y10T436/118339 ,  Y10T436/2575 ,  C07C47/06 ,  C07C47/22 ,  C07C47/04 ,  C07C47/127 ,  C07C49/403 ,  G01N30/466

Abstract: A chemical processing microsystem useful for identifying and optimizing materials (e.g., catalysts) that enhance chemical processes or for characterizing and/or optimizing chemical processes is disclosed. The chemical processing microsystem comprises a plurality of microreactors 600 and, in a preferred embodiment, a plurality of microseparators 900 integral with the chemical processing microsystem 10. The microreactors 600 are preferably diffusion-mixed microreactors formed in a plurality of laminae that include a modular, interchangeable candidate-material array 100. The material array 100 comprises a plurality of different candidate materials (e.g., catalysts), preferably arranged at separate, individually addressable portions of a substrate (e.g., wafer). The microseparators 900 are similarly formed in a plurality of laminae that include a modular, interchangeable adsorbent array 700. The adsorbent array 700 comprises one or more adsorbents, preferably arranged at separate, individually addressable portions of a substrate to spatially correspond to the plurality of different candidate materials. Modular microfluidic distribution systems are also disclosed. The chemical processing microsystem can be integrated into a material evaluation system that enables a comprehensive combinatorial material science research program.

Abstract translation: 公开了一种用于识别和优化增强化学过程或表征和/或优化化学过程的材料（例如催化剂）的化学处理微系统。 化学处理微系统包括多个微反应器600，并且在优选实施例中是与化学处理微系统10整合的多个微分离器900.微反应器600优选地是形成在多个层中的扩散混合微反应器，其包括模块化， 材料阵列100包括多个不同的候选材料（例如，催化剂），优选地布置在基板（例如，晶片）的单独可单独寻址的部分处。 微分离器900类似地形成在包括模块化的可互换的吸附剂阵列700的多个层中。吸附剂阵列700包括一个或多个吸附剂，优选地布置在基板的分开的，可单独寻址的部分上，以在空间上对应于多个不同的 候选材料。 还公开了模块化微流体分布系统。 化学加工微系统可以整合到材料评估系统中，实现全面的组合材料科学研究计划。

公开(公告)号：US06706479B2

公开(公告)日：2004-03-16

申请号：US09870986

申请日：2001-06-01

Applicant: Ravi F. Saraf ,  Sanjun Niu

Inventor： Ravi F. Saraf ,  Sanjun Niu

IPC: C12Q168

CPC classification number: B82Y30/00 ,  B01J2219/00274 ,  B01J2219/00432 ,  B01J2219/00497 ,  B01J2219/00529 ,  B01J2219/00576 ,  B01J2219/00578 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00619 ,  B01J2219/00626 ,  B01J2219/00635 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00707 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00729 ,  C12Q1/6825 ,  C12Q1/6837 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  Y10T428/24917 ,  C12Q2523/319

Abstract: A method detects binding of molecules, advantageously without tagging molecules in the sample. A sensor is used in which is included a single stranded nucleic acid sequence and a photoluminescent material in respective layers. After the sensor is exposed to a biological sample for sufficient time for its single stranded nucleic acid sequence to bind to a material of interest, photoluminescence from the sensor can be measured. An apparatus for tagging-free detection of binding of molecules also is provided. Methods of making tagging-free sensors are provided. Also, tagging-free methods to detect binding of antigens and related devices are disclosed.

Abstract translation: 一种方法检测分子的结合，有利地在标本中标记分子。 使用的传感器包括单层核酸序列和各层中的光致发光材料。 在将传感器暴露于生物样品足够长的时间以使其单链核酸序列与感兴趣的材料结合之后，可以测量来自传感器的光致发光。 还提供了一种用于无标记检测分子结合的装置。 提供无标签传感器的方法。 此外，公开了无标记的方法来检测抗原和相关装置的结合。

公开(公告)号：US20040014077A1

公开(公告)日：2004-01-22

申请号：US10350703

申请日：2003-01-23

Inventor： Peter G. Schultz ,  Xiao-Dong Xiang ,  Isy Goldwasser ,  Gabriel Briceno ,  Xiao-Dong Sun ,  Kai-An Wang

IPC: A61L002/00 ,  B05D003/00 ,  C12Q001/68 ,  G01N033/53 ,  C12M001/34

CPC classification number: C04B35/16 ,  B01J19/0046 ,  B01J2219/00313 ,  B01J2219/00317 ,  B01J2219/00378 ,  B01J2219/0043 ,  B01J2219/00432 ,  B01J2219/00436 ,  B01J2219/00441 ,  B01J2219/00443 ,  B01J2219/00475 ,  B01J2219/005 ,  B01J2219/00511 ,  B01J2219/00515 ,  B01J2219/0052 ,  B01J2219/00527 ,  B01J2219/00531 ,  B01J2219/00533 ,  B01J2219/00536 ,  B01J2219/0054 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00592 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00619 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00628 ,  B01J2219/0063 ,  B01J2219/00635 ,  B01J2219/00637 ,  B01J2219/00644 ,  B01J2219/00646 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00689 ,  B01J2219/00691 ,  B01J2219/00711 ,  B01J2219/0072 ,  B01J2219/00722 ,  B01J2219/00736 ,  B01J2219/00745 ,  B01J2219/00747 ,  B01J2219/00752 ,  B01J2219/00754 ,  B82Y25/00 ,  B82Y30/00 ,  C01G51/006 ,  C01G51/68 ,  C01P2002/34 ,  C01P2002/52 ,  C01P2002/72 ,  C01P2006/40 ,  C01P2006/42 ,  C04B35/01 ,  C04B35/4508 ,  C04B35/4521 ,  C04B35/4525 ,  C04B35/62218 ,  C04B2235/3206 ,  C04B2235/3208 ,  C04B2235/3213 ,  C04B2235/3215 ,  C04B2235/3224 ,  C04B2235/3225 ,  C04B2235/3227 ,  C04B2235/3262 ,  C04B2235/327 ,  C04B2235/3275 ,  C04B2235/3284 ,  C04B2235/3286 ,  C04B2235/3296 ,  C04B2235/3298 ,  C04B2235/3418 ,  C04B2235/3463 ,  C04B2235/656 ,  C04B2235/761 ,  C04B2235/762 ,  C04B2235/768 ,  C04B2235/96 ,  C23C14/042 ,  C23C14/087 ,  C23C16/042 ,  C40B30/08 ,  C40B40/14 ,  C40B40/18 ,  C40B60/08 ,  C40B60/14 ,  C40B70/00 ,  C40B80/00 ,  G01R33/16 ,  H01F1/401 ,  H01F1/407 ,  H01F10/007 ,  H01F10/1933 ,  H01F41/18 ,  H01F41/34 ,  H01L43/10 ,  Y10T428/24479 ,  Y10T428/24612 ,  Y10T428/2462 ,  Y10T428/31 ,  Y10T428/31645 ,  Y10T428/31855 ,  Y10T428/31931 ,  Y10T436/25 ,  Y10T436/2525 ,  Y10T436/255 ,  Y10T436/25875

Abstract: Methods and apparatus for the preparation and use of a substrate having an array of diverse materials in predefined regions thereon. A substrate having an array of diverse materials thereon is generally prepared by delivering components of materials to predefined regions on a substrate, and simultaneously reacting the components to form at least two materials. Materials which can be prepared using the methods and apparatus of the present invention include, for example, covalent network solids, ionic solids and molecular solids. More particularly, materials which can be prepared using the methods and apparatus of the present invention include, for example, inorganic materials, intermetallic materials, metal alloys, ceramic materials, organic materials, organometallic materials, non-biological organic polymers, composite materials (e.g., inorganic composites, organic composites, or combinations thereof), etc. Once prepared, these materials can be screened for useful properties including, for example, electrical, thermal, mechanical, morphological, optical, magnetic, chemical, or other properties. Thus, the present invention provides methods for the parallel synthesis and analysis of novel materials having useful properties.

Abstract translation: 用于制备和使用在其上的预定区域中具有不同材料阵列的基材的方法和装置。 通常通过将材料的组分递送到衬底上的预定区域，并且同时使组分反应以形成至少两种材料来制备其上具有不同材料阵列的衬底。 可以使用本发明的方法和装置制备的材料包括例如共价网络固体，离子固体和分子固体。 更具体地，可以使用本发明的方法和装置制备的材料包括例如无机材料，金属间材料，金属合金，陶瓷材料，有机材料，有机金属材料，非生物有机聚合物，复合材料（例如 ，无机复合材料，有机复合材料或其组合）等。一旦制备，可以对这些材料进行筛选以获得有用的性质，包括例如电，热，机械，形态，光学，磁性，化学或其它性质。 因此，本发明提供了用于并行合成和分析具有有用特性的新型材料的方法。

公开(公告)号：US20040010378A1

公开(公告)日：2004-01-15

申请号：US10430586

申请日：2003-05-06

Applicant: GenTel Corporation

Inventor： Bryce P. Nelson

IPC: G06F019/00

CPC classification number: B82Y30/00 ,  B01J2219/00378 ,  B01J2219/00432 ,  B01J2219/00497 ,  B01J2219/00529 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00626 ,  B01J2219/00635 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00711 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01L3/5027 ,  C12Q1/6837 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  C12Q2522/101

Abstract: The present invention relates to novel methods for the analysis of interactions of transcription factors with target nucleic acids. In particular, the present invention relates to compositions and methods for the detection of transcription factors binding to their target promoter regions. The present invention further provides methods of screening compounds for their ability to alter such binding interactions.

Abstract translation: 本发明涉及用于分析转录因子与靶核酸的相互作用的新方法。 特别地，本发明涉及用于检测结合其靶启动子区的转录因子的组合物和方法。 本发明还提供了筛选化合物改变这种结合相互作用的能力的方法。

公开(公告)号：US20040007694A1

公开(公告)日：2004-01-15

申请号：US10320566

申请日：2002-12-16

Inventor： Xiao-Dong Sun ,  Navjot Singh ,  Lionel Monty Levinson

IPC: H01B001/00

CPC classification number: B01J23/8878 ,  B01J19/0046 ,  B01J23/72 ,  B01J23/80 ,  B01J23/83 ,  B01J23/864 ,  B01J23/866 ,  B01J37/347 ,  B01J2219/00369 ,  B01J2219/00376 ,  B01J2219/0043 ,  B01J2219/00585 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00635 ,  B01J2219/00659 ,  B01J2219/00695 ,  B01J2219/00707 ,  B01J2219/00745 ,  B01J2219/00747 ,  B82Y30/00 ,  C08K7/06 ,  C40B30/08 ,  C40B40/18 ,  C40B60/14 ,  D01F9/127 ,  G01N31/10 ,  Y10S977/742 ,  Y10S977/774 ,  Y10S977/775 ,  Y10S977/881 ,  Y10S977/89 ,  Y10S977/896

Abstract: A catalyst system and method for making carbon fibrils is provided which comprises a catalytic amount of an inorganic catalyst comprising nickel and one of the following substances selected from the group consisting of chromium; chromium and iron; chromium and molybdenum; chromium, molybdenum, and iron; aluminum; yttrium and iron; yttrium, iron and aluminum; zinc; copper; yttrium; yttrium and chromium; and yttrium, chromium and zinc. In a further aspect of the invention, a catalyst system and method is provided for making carbon fibrils which comprises a catalytic amount of an inorganic catalyst comprising cobalt and one of the following substances selected from the group consisting of chromium; aluminum; zinc; copper; copper and zinc; copper, zinc, and chromium; copper and iron; copper, iron, and aluminum; copper and nickel; and yttrium, nickel and copper.

公开(公告)号：US20030232381A1

公开(公告)日：2003-12-18

申请号：US10602900

申请日：2003-06-23

Inventor： Charles S. Vann ,  Tim Geiser ,  Andrew J. Blasband

IPC: C12Q001/68 ,  C12M001/34

CPC classification number: B01L3/5023 ,  B01J19/0046 ,  B01J2219/00326 ,  B01J2219/0043 ,  B01J2219/00495 ,  B01J2219/00515 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00628 ,  B01J2219/0063 ,  B01J2219/00635 ,  B01J2219/00637 ,  B01J2219/00657 ,  B01J2219/00675 ,  B01J2219/00702 ,  B01J2219/00711 ,  B01J2219/00722 ,  B82Y30/00 ,  C12Q1/6816 ,  C40B40/06 ,  C40B50/14 ,  C40B60/14 ,  G01N21/6428 ,  G01N21/645 ,  G01N21/6452 ,  G01N21/648 ,  G01N21/7703 ,  G01N2021/6484 ,  G01N2021/7786 ,  Y10T436/143333 ,  C12Q2565/607

Abstract: An apparatus and method are provided for contacting at least two chemical species, comprising a support plate having a channel for receiving a mobile chemical species and a fiber, having a second chemical species immobilized thereon, disposed on the support plate. At least a portion of the fiber is exposed to the channel such that the mobile chemical species is capable of contacting the second chemical species. An apparatus and method for reading the fiber array, an apparatus and method for making the fiber array, and methods of using the fiber array of the present invention are also provided.

公开(公告)号：US20030207099A1

公开(公告)日：2003-11-06

申请号：US10136181

申请日：2002-05-01

Inventor： Susan D. Gillmor ,  Ferencz S. Denes ,  Max G. Lagally

IPC: C23C016/50 ,  B32B003/24

CPC classification number: B01D67/009 ,  B01D71/70 ,  B01J2219/00387 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00635 ,  B01J2219/00641 ,  B01J2219/00677 ,  B01J2219/00722 ,  B82Y30/00 ,  C40B40/06 ,  C40B60/14 ,  Y10T428/249953

Abstract: A membrane having a stable low-contact angle that is used as a template in forming biological microarrays is provided. The membrane is formed of a polymeric material that has been surface modified by a first plasma treatment and subsequently by a second plasma treatment. The surface modification accomplished by the first plasma treatment results in a significant reduction in the contact angle for the membrane, causing the membrane to become hydrophilic, and the surface modification by the second membrane treatment permanently stabilizes the reduction in the contact angle produced by the first plasma treatment. The resulting membrane allows a solution containing a biological material to wet the surface of the membrane such that the membrane can quickly and easily form a biological microarray on substrate in which the features of the array are distinctly formed on the substrate.

Abstract translation: 提供了一种在形成生物微阵列中用作模板的具有稳定的低接触角的膜。 膜由聚合物材料形成，该聚合材料已经通过第一等离子体处理进行表面改性，随后通过第二等离子体处理。 通过第一等离子体处理实现的表面改性导致膜的接触角显着降低，导致膜变得亲水，并且通过第二膜处理的表面改性永久地稳定了由第一等离子体处理产生的接触角的减小 等离子体处理。 所得到的膜允许含有生物材料的溶液润湿膜的表面，使得膜可以快速且容易地在衬底上形成生物微阵列，其中阵列的特征明显地形成在衬底上。

公开(公告)号：US06638770B1

公开(公告)日：2003-10-28

申请号：US10199968

申请日：2002-07-20

Applicant: Jean I. Montagu

Inventor： Jean I. Montagu

IPC: G01N100

CPC classification number: B01L3/0244 ,  B01J19/0046 ,  B01J2219/00364 ,  B01J2219/00387 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00635 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00691 ,  B01L3/0251 ,  B01L99/00 ,  B82Y30/00 ,  C40B60/14 ,  G01N35/1004 ,  G01N35/1065 ,  G01N2035/1034 ,  G01N2035/1037 ,  G02B21/34 ,  Y10T436/25 ,  Y10T436/2575

Abstract: An apparatus for depositing an array of spots on a receiving surface includes a fluid source, a deposit device that includes a drop-carrying surface, and a cleaning device. The deposit device and a fluid source are cooperatively arranged to deposit a spot(usually created by a drop) on a receiving surface. The cleaning device is constructed and arranged to clean the drop-carrying surface by employing a flow of cleaning fluid or another cleaning matter such as small particles the flow being arranged to prevent contaminating back flow.

Abstract translation: 用于在接收表面上沉积斑点阵列的装置包括流体源，包括落下表面的沉积装置和清洁装置。 沉积装置和流体源协调地布置成在接收表面上沉积点（通常由液滴产生）。 清洁装置被构造和布置成通过使用清洁流体或另一种清洁物质例如小颗粒的流动清洁滴落表面，该流体被布置成防止污染回流。