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88 条记录 (耗时 0.041 秒)

    Method of stabilizing enzyme conjugates
    63.
    发明授权
    Method of stabilizing enzyme conjugates 失效
    稳定酶缀合物的方法

    公开(公告)号：US5686253A

    公开(公告)日：1997-11-11

    申请号：US450744

    申请日：1995-05-25

    申请人： Carl N. Skold ,  Margaret Henson ,  Thomas Michael Houts ,  Ian Gibbons

    发明人： Carl N. Skold ,  Margaret Henson ,  Thomas Michael Houts ,  Ian Gibbons

    IPC分类号： C12N5/10 ,  C12N9/96 ,  C12N15/02 ,  C12N15/09 ,  C12P21/08 ,  C12Q1/26 ,  C12Q1/28 ,  C12Q1/32 ,  C12Q1/68 ,  G01N33/535 ,  G01N33/542 ,  G01N33/573 ,  G01N33/577

    CPC分类号： G01N33/542 ,  C12N9/96 ,  G01N33/535 ,  Y10S435/963 ,  Y10S435/975 ,  Y10S436/822

    摘要： A method is disclosed for stabilizing a conjugate of an enzyme and a member of a specific binding pair (enzyme conjugate). The method comprises the step of combining the enzyme conjugate with an effective amount of an antibody for the enzyme where the antibody does not substantially inhibit the activity of the enzyme. The invention has application to assays for the determination of an analyte wherein enzyme conjugates are employed. The improvement comprises employing as a reagent in the assay an immune complex of an enzyme conjugate and an antibody for the enzyme where the antibody does not substantially inhibit the activity of the enzyme. Compositions comprising such an immune complex and kits comprising such an immune complex in packaged combination with other assay reagents are also disclosed.

    摘要翻译： 公开了用于稳定酶和特异性结合对（酶结合物）成员的缀合物的方法。 该方法包括将酶结合物与酶的有效量的抗体组合的步骤，其中抗体基本上不抑制酶的活性。 本发明应用于测定其中使用酶缀合物的分析物的测定。 该改进包括在测定中使用酶缀合物的免疫复合物和酶的抗体，其中抗体基本上不抑制酶的活性。 还公开了包含这种免疫复合物的组合物和包含与其它测定试剂的包装组合中的这种免疫复合物的试剂盒。

    Capillary mixing device
    64.
    发明授权
    Capillary mixing device 失效
    毛细管混合装置

    公开(公告)号：US5222808A

    公开(公告)日：1993-06-29

    申请号：US867155

    申请日：1992-04-10

    申请人： Jeffrey Sugarman ,  Ian Gibbons

    发明人： Jeffrey Sugarman ,  Ian Gibbons

    IPC分类号： B01F13/00 ,  B01F13/08

    CPC分类号： B01F13/0059 ,  B01F13/0809 ,  B01F15/0232 ,  B01F2215/0037

    摘要： A capillary mixing device, comprising a liquid impervious housing; an interior space in the housing comprising a chamber in the housing having capillary spacing in one dimension and non-capillary spacing in other dimensions; and a plurality of magnetic or magnetically inducible particles in the chamber. The chamber is normally accessed through one or more capillary passageways leading to a surface of the housing and is adapted to be retained by a magnetic device that comprises means for generating a moving magnetic field and means for retaining the chamber device in an orientation so that the magnetic field has a field vector that intersects the capillary chamber perpendicular to the dimension having capillary spacing.

    摘要翻译： 一种毛细管混合装置，包括不透液体的壳体; 壳体中的内部空间包括壳体中的腔室，其中一个维度具有毛细管间隔，在其它尺寸上具有非毛细管间隔; 以及腔室中的多个磁性或磁性诱导性颗粒。 通常通过通过一个或多个毛细管通道进入腔室，该毛细通道通向外壳的表面，并且适于由包括用于产生移动磁场的装置的磁性装置保持，以及用于将腔室装置保持在定向中的装置， 磁场具有与垂直于具有毛细管间隔的尺寸的毛细管腔相交的场向量。

    Apparatus and method for dilution and mixing of liquid samples
    69.
    发明授权
    Apparatus and method for dilution and mixing of liquid samples 失效
    液体样品稀释和混合的装置和方法

    公开(公告)号：US4868129A

    公开(公告)日：1989-09-19

    申请号：US90026

    申请日：1987-08-27

    申请人： Ian Gibbons ,  Robert S. Hillman ,  Channing R. Robertson

    发明人： Ian Gibbons ,  Robert S. Hillman ,  Channing R. Robertson

    IPC分类号： B01F13/00 ,  B01F13/08 ,  B01F15/04 ,  G01N1/38 ,  G01N33/04

    CPC分类号： G01N1/38 ,  B01F13/0059 ,  B01F13/0818 ,  B01F15/04 ,  B01L3/5027 ,  G01N33/04 ,  Y10T436/25625 ,  Y10T436/2575

    摘要： An apparatus for diluting a sample with a diluent, comprising a fixed volume measuring chamber, a fixed volume receiving chamber and fluid receiving relationship to the measuring chamber, a gas vent in the receiving chamber, a stop flow junction between the measuring chamber and the receiving chamber, a sample application site in fluid donating relationship to the measuring chamber, wherein the vertical height difference between the sample application site and the stop flow junction is insufficient to provide a hydrostatic pressure capable of overcoming backpressure at the stop flow junction when sample is applied to the sample application site, and the diluent application site and fluid donating relationship to the measuring chamber. Sample and diluent are added sequentially to the apparatus of the invention without requiring any intervening operations. Various means are provided for restarting flow, which causes diluent to wash the sample into the receiving chamber, where the two can be mixed.

    摘要翻译： 一种用稀释剂稀释样品的装置，包括固定容积测量室，固定容积接收室和与测量室的流体接收关系，接收室中的气体通道，测量室和接收器之间的停止流动接合 室，与测量室的流体供应关系的样品施加部位，其中样品施加部位和止动流动接合点之间的垂直高度差不足以提供当施加样品时能够克服止动流动接合处的背压的静水压力 到样品应用场所，以及稀释剂施用部位和与测量室的流体供给关系。 样品和稀释剂依次添加到本发明的装置中，而不需要任何中间操作。 提供了重新启动流动的各种装置，这使得稀释剂将样品洗涤到接收室中，其中两个可以混合。