公开(公告)号：US20060180609A1

公开(公告)日：2006-08-17

申请号：US10527230

申请日：2003-09-08

Applicant: David Hager ,  Patrick Kearney

Inventor： David Hager ,  Patrick Kearney

IPC: B67D5/56

CPC classification number: G01F11/34 ,  B01J2219/00315 ,  B01J2219/00364 ,  B01J2219/00414 ,  B01J2219/00416 ,  B01J2219/00745 ,  B01J2219/00756 ,  B01L3/021 ,  B01L2200/0657 ,  B01L2300/0681 ,  B01L2300/0829 ,  B01L2400/049 ,  B65B1/38 ,  C40B40/18 ,  C40B60/14 ,  G01N2035/00188

Abstract: The present invention provides apparatus and methods for collecting a plurality of measured quantities of a powdered material and dispensing each of the measured quantities to, for example, a multi-well vessel. Vacuum is used to collect the powdered material and at least one of gravity, a gas push, or a physical push is used to dispense the powdered material. Apparatus and methods of the invention are particularly useful for collection and delivery of powdered materials in high throughput chemical synthesis and biological assay environments where accurate measurement and dispensing of powders is critical.

Abstract translation: 本发明提供了用于收集多个测量量的粉末材料并将每个测量量分配到例如多孔容器的装置和方法。 真空用于收集粉末材料，并且使用重力，气体推动或物理推动中的至少一种来分配粉末材料。 本发明的装置和方法对于在高通量化学合成和生物测定环境中的粉末材料的收集和递送特别有用，其中粉末的精确测量和分配至关重要。

公开(公告)号：US20060088857A1

公开(公告)日：2006-04-27

申请号：US11217194

申请日：2005-09-01

Applicant: Said Attiya ,  Vinod Makhijani ,  Ming Lei ,  Yi-Ju Chen ,  John Simpson ,  G. Roth ,  Chun Ho ,  Yu Pengguang

Inventor： Said Attiya ,  Vinod Makhijani ,  Ming Lei ,  Yi-Ju Chen ,  John Simpson ,  G. Roth ,  Chun Ho ,  Yu Pengguang

IPC: C12Q1/68 ,  C12M1/34

CPC classification number: B01D69/02 ,  B01D61/18 ,  B01D69/10 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00317 ,  B01J2219/00414 ,  B01J2219/00423 ,  B01J2219/00466 ,  B01J2219/005 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00704 ,  B01J2219/00722 ,  B01L3/50255 ,  B01L2300/0819 ,  B01L2300/0877 ,  C12Q1/6869 ,  C12Q2565/301 ,  C12Q2565/501

Abstract: The present invention relates to methods and apparatuses for conducting densely packed, independent chemical reactions in parallel in fluid-permeable arrays. Accordingly, this invention also focuses on the use of such arrays for applications such as DNA sequencing, most preferably pyrophosphate sequencing, and DNA amplification.

Abstract translation: 本发明涉及用于在流体可渗透阵列中并行进行密集堆积的独立化学反应的方法和装置。 因此，本发明还集中于这种阵列用于诸如DNA测序，最优选焦磷酸测序和DNA扩增的应用。

公开(公告)号：US06932939B2

公开(公告)日：2005-08-23

申请号：US10855198

申请日：2004-05-27

Applicant: Can Özbal ,  Ian W. Hunter ,  John Linton

Inventor： Can Özbal ,  Ian W. Hunter ,  John Linton

IPC: G01N27/62 ,  B01F13/00 ,  B01J19/00 ,  B01L3/00 ,  B01L3/02 ,  C40B60/14 ,  G01N1/00 ,  G01N1/36 ,  G01N21/33 ,  G01N21/64 ,  G01N21/65 ,  G01N21/77 ,  G01N21/78 ,  G01N30/16 ,  G01N30/24 ,  G01N30/46 ,  G01N30/62 ,  G01N30/72 ,  G01N30/84 ,  G01N35/00 ,  G01N35/10 ,  B01D15/08

CPC classification number: G01N30/466 ,  B01F5/0085 ,  B01F13/0059 ,  B01J19/0046 ,  B01J2219/0036 ,  B01J2219/00387 ,  B01J2219/00414 ,  B01J2219/00418 ,  B01J2219/00479 ,  B01J2219/00495 ,  B01J2219/00515 ,  B01J2219/00518 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00657 ,  B01J2219/00659 ,  B01J2219/00689 ,  B01J2219/00702 ,  B01J2219/0072 ,  B01L3/0262 ,  B01L3/0268 ,  B01L3/505 ,  B01L3/5085 ,  B01L2300/0812 ,  B01L2300/0845 ,  C40B60/14 ,  G01N30/24 ,  G01N30/7233 ,  G01N30/7266 ,  G01N30/84 ,  G01N35/00009 ,  G01N35/1095 ,  G01N2030/628 ,  G01N2030/8417 ,  G01N2035/1037 ,  G01N2035/1046 ,  Y10T436/115831 ,  G01N30/18

Abstract: A system and method high throughput sample preparation and analysis using column chromatography. Each port in a plurality of ports has a port input that interfaces with a first fluid source and a port output. A fluidic circuit is coupled to each port output and to a second fluid source, the fluidic circuit for controlling fluid flow from the plurality of ports and the second fluid source. The fluidic circuit is also coupled to a plurality of chromatography columns. An interface to an analyzer receives output from at least one of the plurality of chromatography columns. The plurality of chromatography columns is moved relative to the analyzer via a translation stage, such that sample output from one of the plurality of chromatography columns can be selectively presented to the analyzer.

Abstract translation: 一种使用柱色谱法的高通量样品制备和分析的系统和方法。 多个端口中的每个端口具有与第一流体源和端口输出接口的端口输入。 流体回路耦合到每个端口输出端和第二流体源，流体回路用于控制来自多个端口和第二流体源的流体流。 流体回路还耦合到多个色谱柱。 分析仪的界面接收至少一个色谱柱的输出。 多个色谱柱经由平移台相对于分析器移动，使得从多个色谱柱中的一个色谱柱输出的样品可以选择性地呈现给分析器。

公开(公告)号：US20050084423A1

公开(公告)日：2005-04-21

申请号：US10901942

申请日：2004-07-28

Applicant: Michael Zarowitz ,  Glenn Edwards

Inventor： Michael Zarowitz ,  Glenn Edwards

IPC: B01L3/02

CPC classification number: G01N35/1011 ,  B01J2219/00364 ,  B01J2219/00414 ,  B01J2219/00423 ,  B01J2219/00466 ,  B01J2219/005 ,  B01L3/021 ,  B01L3/5085 ,  B01L2200/025 ,  B01L2200/0642 ,  B01L2200/0647 ,  B01L2200/0657 ,  B01L2200/143 ,  B01L2300/0627 ,  B01L2300/0681 ,  B01L2300/0829 ,  B01L2300/0851 ,  B01L2300/0867 ,  B01L2300/0887 ,  B01L2300/168 ,  B01L2400/0487 ,  C12M33/04 ,  G01N35/026

Abstract: Systems, including methods and apparatus, for particle manipulation to perform assays.

Abstract translation: 用于进行测定的粒子操作的系统，包括方法和装置。

公开(公告)号：US20050048205A1

公开(公告)日：2005-03-03

申请号：US10852792

申请日：2004-05-24

Applicant: Seong-Ihl Woo ,  Ki-Woong Kim

Inventor： Seong-Ihl Woo ,  Ki-Woong Kim

IPC: B01J29/76 ,  B01J19/00 ,  B01J19/10 ,  B05D1/02 ,  B05D7/24 ,  C23C16/00 ,  C23C16/44 ,  C23C18/06 ,  C23C18/08 ,  C23C18/12 ,  H01L21/20 ,  H01L21/316 ,  H01M4/139 ,  H01M4/88 ,  H01M4/92 ,  H01M10/05

CPC classification number: H01M4/13 ,  B01J19/0046 ,  B01J2219/00414 ,  B01J2219/0043 ,  B01J2219/00495 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00659 ,  B01J2219/00747 ,  B01J2219/0075 ,  B01J2219/00754 ,  C23C18/06 ,  C23C18/08 ,  C23C18/1216 ,  C23C18/1225 ,  C23C18/1279 ,  H01M4/139 ,  H01M4/485 ,  H01M4/505 ,  H01M4/525 ,  H01M4/886 ,  H01M4/8867 ,  H01M4/921

Abstract: The present invention concerns a method of providing a wet deposition process with a shutter driven in one axis direction in order to produce a thin film or powder array various in composition on a wafer or in a reactor having apertures as many as the number of sample to be produced. A material having various compositions is transferred to an area predetermined by means of a mask on the wafer to form an array having minimum 16 to about 20000 different compositions by mixture or reaction of at least two or more materials to a minimum in a liquid state. By the process, it is possible to develop materials for various use, e.g., ferroelectrics and inorganic material including fluorescencers, organic polymers, organic metals, ionic solids and metal alloys, more efficiently than by the current experiment. The invention also comprises a method of characteristic analysis of the aforementioned array within a short time, in addition to development of the array having aforementioned various compositions.

Abstract translation: 本发明涉及一种提供湿式沉积工艺的方法，该方法具有沿一个轴向驱动的快门，以便在晶片或反应器中制备各种成分的薄膜或粉末阵列，该反应器的孔径与样品数量一样多 生产。 将具有各种组成的材料通过掩模在晶片上转移到预定的区域，以通过至少两种或更多种材料的混合物或反应在液态下形成具有最小16至约20000种不同组成的阵列至最小。 通过该过程，可以比通过当前实验更有效地开发各种用途的材料，例如铁电体和无机材料，包括荧光灯，有机聚合物，有机金属，离子固体和金属合金。 除了具有上述各种组成的阵列的开发之外，本发明还包括在短时间内对上述阵列进行特征分析的方法。

公开(公告)号：US20050009022A1

公开(公告)日：2005-01-13

申请号：US10482491

申请日：2002-07-08

Applicant: Michael Weiner ,  Said Attiya ,  Hugh Crenshaw ,  Jonathan Rothberg ,  Stephen Matson

Inventor： Michael Weiner ,  Said Attiya ,  Hugh Crenshaw ,  Jonathan Rothberg ,  Stephen Matson

IPC: C12N15/09 ,  B01D61/14 ,  B01D71/56 ,  B01J19/00 ,  C12M1/34 ,  C12M1/40 ,  C12Q1/68 ,  C12Q1/6869 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14

CPC classification number: B01J19/0046 ,  B01J2219/00283 ,  B01J2219/00286 ,  B01J2219/00313 ,  B01J2219/00355 ,  B01J2219/00414 ,  B01J2219/00418 ,  B01J2219/00423 ,  B01J2219/00466 ,  B01J2219/00497 ,  B01J2219/005 ,  B01J2219/00524 ,  B01J2219/00576 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00641 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00702 ,  B01J2219/00722 ,  B01J2219/00725 ,  C12Q1/6869 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14

Abstract: The present invention relates to the field of fluid dynamics. More specifically, this invention relates to methods and apparatus for conducting densely packed, independent chemical reactions in parallel in a substantially two-dimensional array. Accordingly, this invention also focuses on the use of this array for applications such as DNA sequencing, most preferably pyrosequencing, and DNA amplification.

Abstract translation: 本发明涉及流体动力学领域。 更具体地，本发明涉及用于以基本上二维阵列并行进行密集包装的独立化学反应的方法和装置。 因此，本发明还着重于将该阵列用于诸如DNA测序，最优选焦磷酸测序和DNA扩增的应用。

公开(公告)号：US20030232140A1

公开(公告)日：2003-12-18

申请号：US10172470

申请日：2002-06-14

Inventor： Joseph Remick ,  Anthony J.D. Feurtado ,  Maryam Mobed-Miremadi ,  Tim J. Lazaruk ,  Jay K. Bass

IPC: B05D003/00

CPC classification number: B82Y30/00 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00378 ,  B01J2219/00385 ,  B01J2219/00414 ,  B01J2219/00418 ,  B01J2219/00432 ,  B01J2219/00497 ,  B01J2219/00518 ,  B01J2219/00527 ,  B01J2219/00536 ,  B01J2219/00538 ,  B01J2219/00542 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00637 ,  B01J2219/00657 ,  B01J2219/00659 ,  B01J2219/00662 ,  B01J2219/00664 ,  B01J2219/00675 ,  B01J2219/00677 ,  B01J2219/00689 ,  B01J2219/00691 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00729 ,  B01J2219/00731 ,  C40B40/06 ,  C40B40/10 ,  C40B40/12 ,  C40B50/14 ,  C40B60/14

Abstract: Methods and apparatus are disclosed for synthesizing a plurality of compounds on the surface of supports. Biopolymer features are attached to the surfaces of the supports. The synthesis generally comprises a plurality of steps. The support is placed into a flow chamber, and a reagent is introduced into the flow chamber. The reagent is reactive with features on the surface of the support. During removal of the reagent from the flow chamber, the pressure in the chamber is maintained substantially atmospheric. In another embodiment the reagent is removed from the flow chamber under vacuum. In another embodiment the reagent is removed from the flow chamber by simultaneously venting and applying a vacuum to the flow chamber.

Abstract translation: 公开了用于在支撑体的表面上合成多种化合物的方法和装置。 生物聚合物特征附着在支撑体的表面上。 合成通常包括多个步骤。 将载体置于流动室中，并将试剂引入流动室中。 试剂与载体表面上的特征反应。 在从流动室移除试剂期间，室中的压力基本保持为大气压。 在另一个实施方案中，试剂在真空下从流动室中除去。 在另一个实施方案中，通过同时排出并向流动室施加真空，将试剂从流动室中移出。

公开(公告)号：US20030082081A1

公开(公告)日：2003-05-01

申请号：US10277754

申请日：2002-10-23

Applicant: COMMISSARIAT A L'ENERGIE ATOMIQUE

Inventor： Yves Fouillet ,  Nicolas Sarrut ,  Antoine Gruss

IPC: B01L003/00 ,  G05D001/00

CPC classification number: B01F13/0071 ,  B01F13/0076 ,  B01F13/0077 ,  B01F15/00123 ,  B01F15/00344 ,  B01J19/0093 ,  B01J2219/00286 ,  B01J2219/00306 ,  B01J2219/00353 ,  B01J2219/00389 ,  B01J2219/00414 ,  B01J2219/00418 ,  B01J2219/00495 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00657 ,  B01J2219/00659 ,  B01J2219/0068 ,  B01J2219/00783 ,  B01J2219/00873 ,  B01J2219/00891 ,  B01J2219/0093 ,  B01L3/563 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2300/1822 ,  B01L2400/0403 ,  B01L2400/0475 ,  B01L2400/06 ,  B01L2400/082 ,  C40B60/14 ,  G01N35/08 ,  G01N2035/00237 ,  Y10T137/0391

Abstract: The invention relates to a microfluidic device for injecting series of mobile reaction chambers (102, 103) having non-miscible segmenters (101) in microchannels (21 to 26), comprising: injection means (10) for injecting into microreaction channels alternatingly and in parallel liquid to form mobile reaction chambers and liquid for forming the segmenters; means for controlling the progression of one of the two liquids, applied to act on one zone (31) of each microchannel delimiting an injection volume of said liquid; the control means being able to cause stopping or slowing of the progression of said liquid over the zone of each microchannel by exerting an action based on a physico-chemical property of the liquid and said action not affecting the other liquid.

Abstract translation: 本发明涉及一种用于在微通道（21至26）中注入具有不可混合分段器（101）的系列移动反应室（102,103）的微流体装置，包括：注入装置（10），用于交替地和在 平行液体形成流动反应室和用于形成分段器的液体; 用于控制两个液体中的一个液体的进行的装置，用于作用于限定所述液体的喷射体积的每个微通道的一个区域（31）; 所述控制装置能够通过基于液体的物理化学性质和不影响其它液体的作用施加作用而使所述液体在每个微通道的区域上的进展停止或减慢。

公开(公告)号：US20030032035A1

公开(公告)日：2003-02-13

申请号：US10139100

申请日：2002-05-03

Applicant: COMMISSARIAT A L'ENERGIE ATOMIQUE

Inventor： Francois Chatelain ,  Felix W. Frueh

IPC: C12Q001/68 ,  G01N033/53 ,  G01N033/542 ,  C12M001/34

CPC classification number: B01L3/5027 ,  B01J2219/00274 ,  B01J2219/00286 ,  B01J2219/00358 ,  B01J2219/00367 ,  B01J2219/00414 ,  B01J2219/00418 ,  B01J2219/00454 ,  B01J2219/00495 ,  B01J2219/00511 ,  B01J2219/0052 ,  B01J2219/00587 ,  B01J2219/0059 ,  B01J2219/00722 ,  B01L3/502707 ,  B01L3/527 ,  B01L7/00 ,  B01L7/52 ,  B01L2200/16 ,  B01L2300/0838 ,  B01L2300/16 ,  B01L2300/18 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0487 ,  C40B40/06 ,  C40B50/14 ,  C40B60/14 ,  G01N33/54366

Abstract: The invention relates to a microfluidic device for nucleic acid and/or protein analysis, wherein said device comprises a capillary on the inner surface of which is attached an array of at least two reagents. Also encompassed by the invention is such a capillary. In addition, the invention concerns methods for attaching at least two reagents on the inner surface of a capillary, as well as methods using a microfluidic device and enabling a multiplex analysis of nucleic acids and/or proteins to be performed.

Abstract translation: 本发明涉及一种用于核酸和/或蛋白质分析的微流体装置，其中所述装置包括毛细管，其内表面附接有至少两种试剂的阵列。 本发明还包括这样的毛细管。 此外，本发明涉及在毛细管的内表面上附着至少两种试剂的方法，以及使用微流体装置并能够进行核酸和/或蛋白质多重分析的方法。

公开(公告)号：US06277332B1

公开(公告)日：2001-08-21

申请号：US09049245

申请日：1998-03-27

Applicant: Irving Sucholeiki

Inventor： Irving Sucholeiki

IPC: B01F1102

CPC classification number: B01F11/0283 ,  B01F13/0809 ,  B01J19/0046 ,  B01J2219/00283 ,  B01J2219/00308 ,  B01J2219/00328 ,  B01J2219/00351 ,  B01J2219/00414 ,  B01J2219/00454 ,  B01J2219/00459 ,  B01J2219/00486 ,  B01J2219/00495 ,  B01J2219/005 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00686 ,  B01J2219/00691 ,  B01J2219/0072 ,  B01J2219/00725 ,  B06B3/02 ,  C07K1/042 ,  C07K1/045 ,  C40B40/10 ,  C40B50/14 ,  C40B60/14 ,  Y10T436/113332

Abstract: The inventive apparatus is comprised of a means for producing cavitation in a complex reaction mixture to enhance the yield of the selected reaction product, a means for controlling the temperature of the complex reaction mixture, especially during cavitation, and a means for affecting magnetic separation of paramagnetic beads to which the selected reaction product is attached from the complex reaction mixture. In one embodiment, the compound(s) or molecule(s) is synthesized in situ, and isolated using the inventive apparatus. The apparatus finds use in the fields of solid phase organic synthesis, and for isolation and purification of a selected compound(s) or molecule (s), especially where automation is desired.

Abstract translation: 本发明的装置包括用于在复合反应混合物中产生空化以提高所选择的反应产物的产率的装置，特别是在空化期间控制复合反应混合物的温度的手段和用于影响磁分离的装置 选择的反应产物从复合反应混合物连接到其上的顺磁珠。 在一个实施方案中，化合物或分子原位合成，并使用本发明的装置分离。 该装置用于固相有机合成领域，并用于选择的化合物或分子的分离和纯化，特别是在需要自动化的情况下。