-
公开(公告)号：US20120040844A1
公开(公告)日：2012-02-16
申请号：US13022694
申请日：2011-02-08
Applicant: Jonathan M. Rothberg , Wolfgang Hinz , Kim L. Johnson , James Bustillo
Inventor： Jonathan M. Rothberg , Wolfgang Hinz , Kim L. Johnson , James Bustillo
CPC classification number: G01N27/4148 , C12Q1/6818 , C12Q1/6874 , G01N27/4145 , G01N33/54373 , G01N33/5438 , H01L27/088 , H01L29/42324 , H01L29/78 , H01L2924/01006 , H01L2924/01013 , H01L2924/01015 , H01L2924/01073 , H01L2924/14 , H01L2924/1433 , Y10T436/22 , C12Q2565/607 , C12Q2565/301 , C12Q2533/101
Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis.
-
公开(公告)号：US20120037961A1
公开(公告)日：2012-02-16
申请号：US13022678
申请日：2011-02-08
Applicant: Jonathan M. Rothberg , Wolfgang Hinz , Kim L. Johnson , James Bustillo
Inventor： Jonathan M. Rothberg , Wolfgang Hinz , Kim L. Johnson , James Bustillo
IPC: H01L29/788 , H01L21/336
CPC classification number: G01N27/4148 , C12Q1/6818 , C12Q1/6874 , G01N27/4145 , G01N33/54373 , G01N33/5438 , H01L27/088 , H01L29/42324 , H01L29/78 , H01L2924/01006 , H01L2924/01013 , H01L2924/01015 , H01L2924/01073 , H01L2924/14 , H01L2924/1433 , Y10T436/22 , C12Q2565/607 , C12Q2565/301 , C12Q2533/101
Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis.
-
公开(公告)号：US20110251078A1
公开(公告)日：2011-10-13
申请号：US13044330
申请日：2011-03-09
Applicant: Jonathan M. Rothberg , John H. Leamon , John F. Davidson , Antoine M. van Oijen , Wolfgang Hinz , Melville Davey , Bradley Hann , Jonathan Schultz
Inventor： Jonathan M. Rothberg , John H. Leamon , John F. Davidson , Antoine M. van Oijen , Wolfgang Hinz , Melville Davey , Bradley Hann , Jonathan Schultz
IPC: C40B30/00
CPC classification number: C12Q1/6874 , C12Q2523/303 , C12Q2565/131 , C12Q2525/204 , C12Q2521/101 , C12Q2535/122
Abstract: The invention provides apparatuses and methods of use thereof for sequencing nucleic acids subjected to a force, and thus considered under tension. The methods may employ but are not dependent upon incorporation of extrinsically detectably labeled nucleotides.
-
公开(公告)号：US20110247933A1
公开(公告)日：2011-10-13
申请号：US13036759
申请日：2011-02-28
Applicant: Jonathan M. ROTHBERG , Wolfgang HINZ
Inventor： Jonathan M. ROTHBERG , Wolfgang HINZ
IPC: G01N27/414 , G01N33/00
CPC classification number: C12Q1/6869 , C12Q1/6874 , G01N27/414 , G01N27/4145 , G01N27/4148 , H01L21/306 , H01L24/18 , H01L24/20 , H01L24/82 , H01L27/088 , H01L29/78 , H01L2224/04105 , H01L2224/16 , H01L2224/76155 , H01L2224/82102 , H01L2924/01005 , H01L2924/01006 , H01L2924/01011 , H01L2924/01012 , H01L2924/01013 , H01L2924/01015 , H01L2924/01019 , H01L2924/0102 , H01L2924/01023 , H01L2924/01024 , H01L2924/01027 , H01L2924/01029 , H01L2924/0103 , H01L2924/01033 , H01L2924/01046 , H01L2924/01047 , H01L2924/01052 , H01L2924/01056 , H01L2924/01059 , H01L2924/01073 , H01L2924/01074 , H01L2924/01075 , H01L2924/01082 , H01L2924/1306 , H01L2924/13091 , H01L2924/14 , H01L2924/1433 , H01L2924/19041 , H01L2924/19042 , H01L2924/19043 , H01L2924/30105 , H01L2924/3011 , H01L2924/3025 , C12Q2565/607 , C12Q2565/301 , C12Q2533/101 , H01L2924/00
Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis.
-
35.发明申请Microfluidic Devices and Methods of Use in The Formation and Control of Nanoreactors 审中-公开微流体装置及其在纳米反应器的形成与控制中的应用公开(公告)号：US20100137163A1
公开(公告)日：2010-06-03
申请号：US12087713
申请日：2006-06-01
Applicant: Darren R. Link , Laurent Boitard , Jeffrey Branciforte , Yves Charles , Gilbert Feke , John Q. Lu , David Marran , Ahmadali Tabatabai , Michael Weiner , Wolfgang Hinz , Jonathan M. Rothberg
Inventor： Darren R. Link , Laurent Boitard , Jeffrey Branciforte , Yves Charles , Gilbert Feke , John Q. Lu , David Marran , Ahmadali Tabatabai , Michael Weiner , Wolfgang Hinz , Jonathan M. Rothberg
CPC classification number: B01F3/0807 , B01F13/0071 , B01F13/0076 , B01J19/0046 , B01J2219/00286 , B01J2219/00459 , B01J2219/00466 , B01J2219/00468 , B01J2219/00479 , B01J2219/005 , B01J2219/00576 , B01J2219/00599 , B01J2219/00702 , B01J2219/0072 , B01J2219/00743 , B01L3/5027 , B01L3/502715 , B01L3/50273 , B01L3/502746 , B01L3/502761 , B01L3/502784 , B01L3/502792 , B01L3/565 , B01L7/52 , B01L2200/027 , B01L2200/0621 , B01L2200/0647 , B01L2200/0652 , B01L2200/0673 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/0896 , B01L2400/0415 , B01L2400/0424 , B01L2400/0487 , B01L2400/084 , B82Y5/00 , B82Y30/00 , C07K1/047 , C12N15/1075 , C12Q1/6804 , C12Q1/6816 , C12Q1/6825 , C12Q1/6834 , C12Q1/686 , C12Q1/6869 , C12Q1/6874 , C12Q1/6876 , C12Q2525/161 , C12Q2561/119 , C12Q2563/103 , C12Q2563/107 , C12Q2563/159 , C12Q2565/537 , C12Q2565/629 , C12Q2600/16 , C40B30/04 , C40B40/04 , C40B50/08 , C40B60/08 , C40B60/12 , C40B70/00 , G01N21/6428 , G01N21/6445 , G01N21/6458 , G01N33/536 , G01N33/542 , G01N33/543 , G01N33/6845 , G01N33/6854 , G01N2500/10
Abstract: The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. Such methods can include labeling a library of compounds by emulsifying aqueous solutions of the compounds and aqueous solutions of unique liquid labels on a microfluidic device, which includes a plurality of electrically addressable, channel bearing fluidic modules integrally arranged on a microfabricated substrate such that a continuous channel is provided for flow of immiscible fluids, whereby each compound is labeled with a unique liquid label, pooling the labeled emulsions, coalescing the labeled emulsions with emulsions containing a specific cell or enzyme, thereby forming a nanoreactor, screening the nanoreactors for a desirable reaction between the contents of the nanoreactor, and decoding the liquid label, thereby identifying a single compound from a library of compounds.
Abstract translation: 本发明提供了可用于进行高通量筛选测定和组合化学的新型微流体装置和方法。 这样的方法可以包括通过将化合物的水溶液和独特的液体标签的水溶液乳化在微流体装置上来标记化合物文库，所述微流体装置包括整体地布置在微细加工的基底上的多个可电寻址的通道支承的流体模块， 提供了用于不混溶流体流动的通道，由此每种化合物用独特的液体标记标记，汇集标记的乳液，将标记的乳液与含有特定细胞或酶的乳液聚结，从而形成纳米反应器，筛选纳米反应器以获得所需的反应 在纳米反应器的内容物之间，并解码液体标签，从而从化合物文库鉴定单一化合物。
-
Patent Agency Ranking
公开(公告)号：US20100035252A1
公开(公告)日：2010-02-11
申请号：US12319140
申请日：2008-12-31
Applicant: Jonathan M. Rothberg , John H. Leamon , John F. Davidson , Antoine M. Van Oijen , Wolfgang Hinz , Melville Davey , Bradley Hann , Jonathan Schultz
Inventor： Jonathan M. Rothberg , John H. Leamon , John F. Davidson , Antoine M. Van Oijen , Wolfgang Hinz , Melville Davey , Bradley Hann , Jonathan Schultz
CPC classification number: C12Q1/6874 , C12Q2523/303 , C12Q2565/131 , C12Q2525/204 , C12Q2521/101 , C12Q2535/122
Abstract: The invention provides apparatuses and methods of use thereof for sequencing nucleic acids subjected to a force, and thus considered under tension. The methods may employ but are not dependent upon incorporation of extrinsically detectably labeled nucleotides.
Abstract translation: 本发明提供了用于测序经受力的核酸的装置及其使用方法，并因此在张力下考虑。 所述方法可以采用但不依赖于外源可检测标记的核苷酸的引入。
-
公开(公告)号：USD602785S1
公开(公告)日：2009-10-27
申请号：US29325007
申请日：2008-09-24
Applicant: Jonathan M. Rothberg , Wolfgang Hinz
Designer： Jonathan M. Rothberg , Wolfgang Hinz
-
公开(公告)号：USD602784S1
公开(公告)日：2009-10-27
申请号：US29325009
申请日：2008-09-24
Applicant: Jonathan M. Rothberg , Wolfgang Hinz
Designer： Jonathan M. Rothberg , Wolfgang Hinz
-
公开(公告)号：US20090011959A1
公开(公告)日：2009-01-08
申请号：US12154901
申请日：2008-05-27
Applicant: Gina L. Costa , John H. Leamon , Jonathan M. Rothberg , Michael P. Weiner
Inventor： Gina L. Costa , John H. Leamon , Jonathan M. Rothberg , Michael P. Weiner
IPC: C40B50/14
CPC classification number: C12Q1/686 , B01L3/5027 , B01L3/502707 , B01L3/502715 , B01L3/5085 , B01L7/52 , B01L2300/0636 , B01L2300/0819 , B01L2300/0877 , C07H21/00 , C12N15/1075 , C12N15/1093 , C12Q1/6806 , C12Q1/6834 , C12Q1/6844 , C12Q1/6865 , C12Q1/6867 , C12Q1/6869 , C12Q1/6874 , G01N21/253 , G01N21/6428 , G01N21/6452 , G01N21/6458 , G01N2021/6484 , G01R33/1269 , Y02P20/582 , Y10T436/143333 , C12Q2565/119 , C12Q2531/113 , C12Q2565/537 , C12Q2547/107 , C12Q2547/101 , C12Q2537/143 , C12Q2527/125 , C12Q2525/186 , C12Q2565/301 , C12Q2563/149 , C12Q2531/125 , C12Q2537/149 , C12Q2535/119
Abstract: This invention relates to methods of generating single stranded DNA libraries for use in amplification and sequencing reactions. In various aspects, the disclosed methods include: fragmenting DNA; polishing the fragments' ends; ligating the fragments to universal adaptors; performing strand displacement and extension of the nicked fragments; purifying the double-stranded ligation products; capturing the double-stranded ligation products onto a solid support; and isolating single stranded DNA library fragments, and binding these fragments to another solid support.
Abstract translation: 本发明涉及产生用于扩增和测序反应的单链DNA文库的方法。 在各方面，所公开的方法包括：片段化DNA; 抛光片段的末端; 将碎片连接到通用适配器; 进行缝合位移和切口碎片的延伸; 纯化双链结合产物; 将双链结合产物捕获到固体支持物上; 并分离单链DNA文库片段，并将这些片段结合到另一种固体支持物上。
-
公开(公告)号：US07323305B2
公开(公告)日：2008-01-29
申请号：US10767779
申请日：2004-09-22
Applicant: John H. Leamon , Kenton L. Lohman , Jonathan M. Rothberg , Michael P. Weiner
Inventor： John H. Leamon , Kenton L. Lohman , Jonathan M. Rothberg , Michael P. Weiner
CPC classification number: C12Q1/686 , B01L3/5027 , B01L3/502707 , B01L3/502715 , B01L3/5085 , B01L7/52 , B01L2300/0636 , B01L2300/0819 , B01L2300/0877 , C07H21/00 , C12N15/1075 , C12N15/1093 , C12Q1/6806 , C12Q1/6834 , C12Q1/6844 , C12Q1/6865 , C12Q1/6867 , C12Q1/6869 , C12Q1/6874 , G01N21/253 , G01N21/6428 , G01N21/6452 , G01N21/6458 , G01N2021/6484 , G01R33/1269 , Y02P20/582 , Y10T436/143333 , C12Q2565/119 , C12Q2531/113 , C12Q2565/537 , C12Q2547/107 , C12Q2547/101 , C12Q2537/143 , C12Q2527/125 , C12Q2525/186 , C12Q2565/301 , C12Q2563/149 , C12Q2531/125 , C12Q2537/149 , C12Q2535/119
Abstract: An apparatus and method for performing rapid DNA sequencing, such as genomic sequencing, is provided herein. The method includes the steps of preparing a sample DNA for genomic sequencing, amplifying the prepared DNA in a representative manner, and performing multiple sequencing reaction on the amplified DNA with only one primer hybridization step.
Abstract translation: 本文提供了用于进行快速DNA测序的装置和方法，例如基因组测序。 该方法包括以下步骤：制备用于基因组测序的样品DNA，以代表性方式扩增所制备的DNA，并仅在一个引物杂交步骤对经扩增的DNA进行多次测序反应。
-
-
-
-
-
-
-
-
-
Search Results
131
records
(took 0.048 seconds)
