-
公开(公告)号：US10139411B2
公开(公告)日：2018-11-27
申请号：US15415290
申请日：2017-01-25
IPC分类号： G01N33/58 , G01N33/573 , B01F5/06 , B01J19/00 , B01F13/00 , B01L3/00 , B01F3/08 , C40B40/04 , C40B50/08 , C40B60/10 , G01N33/50 , C12Q1/25 , C12Q1/6804 , C12Q1/6818 , C12Q1/6827 , G01N33/542 , B01L7/00 , G01N21/64
摘要： The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number.
-
公开(公告)号：US09944977B2
公开(公告)日：2018-04-17
申请号：US14568923
申请日：2014-12-12
发明人： Darren R. Link , Michael Samuels
CPC分类号： C12Q1/6844 , C12Q1/6858 , C12Q2535/122 , C12Q2563/159 , C12Q2563/179 , C12Q2565/629
摘要： The invention generally relates to methods for distinguishing a rare genetic variation in a nucleic acid sequence.
-
公开(公告)号：US09733168B2
公开(公告)日：2017-08-15
申请号：US15082424
申请日：2016-03-28
发明人： Benjamin Miller , Brian Hutchison , Andrew Wilson , Jonathan William Larson , Qun Zhong , Yevgeny Yurkovetsky , Darren Link , Mark Weary
CPC分类号： G01N1/38 , B01L3/0241 , B01L3/502715 , B01L3/502784 , B01L2200/061 , B01L2200/0673 , B01L2200/143 , B01L2300/0877 , F16K99/0001 , F16K2099/0084 , F17D3/01 , G01N27/44791 , G01N2021/0353 , Y10T137/2185 , Y10T137/7761 , Y10T137/8158 , Y10T137/8593 , Y10T436/118339
摘要： The invention provides methods for assessing one or more predetermined characteristics or properties of a microfluidic droplet within a microfluidic channel, and regulating one or more fluid flow rates within that channel to selectively alter the predetermined microdroplet characteristic or property using a feedback control.
-
4.发明授权Microfluidic devices and methods of use in the formation and control of nanoreactors 有权微流体装置和用于形成和控制纳米反应器的方法公开(公告)号：US09534216B2
公开(公告)日：2017-01-03
申请号：US14248991
申请日：2014-04-09
发明人： Darren R. Link , Laurent Boitard , Jeffrey Branciforte , Yves Charles , Gilbert Feke , John Q. Lu , David Marran , Ahmadali Tabatabai , Michael Weiner , Wolfgang Hinz , Jonathan M. Rothberg
IPC分类号： G01N15/10 , C12Q1/68 , G01N21/00 , G01N21/21 , C12N15/10 , B01F13/00 , B01J19/00 , B82Y5/00 , B82Y30/00 , C07K1/04 , C40B30/04 , C40B40/04 , C40B50/08 , C40B60/08 , C40B60/12 , C40B70/00 , G01N33/536 , G01N33/68 , G01N33/543 , G01N33/50 , G01N33/00 , B01L3/00 , B01F3/08 , G01N33/542 , G01N21/64 , B01L7/00
CPC分类号： C12N15/1075 , B01F3/0807 , B01F13/0071 , B01F13/0076 , B01J19/0046 , B01J2219/00286 , B01J2219/00459 , B01J2219/00466 , B01J2219/00468 , B01J2219/00479 , B01J2219/005 , B01J2219/00576 , B01J2219/00599 , B01J2219/00702 , B01J2219/0072 , B01J2219/00743 , B01L3/5027 , B01L3/502715 , B01L3/50273 , B01L3/502746 , B01L3/502761 , B01L3/502784 , B01L3/502792 , B01L3/565 , B01L7/52 , B01L2200/027 , B01L2200/0621 , B01L2200/0647 , B01L2200/0652 , B01L2200/0673 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/0896 , B01L2400/0415 , B01L2400/0424 , B01L2400/0487 , B01L2400/084 , B82Y5/00 , B82Y30/00 , C07K1/047 , C12Q1/6804 , C12Q1/6816 , C12Q1/6825 , C12Q1/6834 , C12Q1/686 , C12Q1/6869 , C12Q1/6874 , C12Q1/6876 , C12Q2525/161 , C12Q2561/119 , C12Q2563/103 , C12Q2563/107 , C12Q2563/159 , C12Q2565/537 , C12Q2565/629 , C12Q2600/16 , C40B30/04 , C40B40/04 , C40B50/08 , C40B60/08 , C40B60/12 , C40B70/00 , G01N21/6428 , G01N21/6445 , G01N21/6458 , G01N33/536 , G01N33/542 , G01N33/543 , G01N33/6845 , G01N33/6854 , G01N2500/10
摘要： The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. The invention provides for aqueous based emulsions containing uniquely labeled cells, enzymes, nucleic acids, etc., wherein the emulsions further comprise primers, labels, probes, and other reactants. An oil based carrier-fluid envelopes the emulsion library on a microfluidic device, such that a continuous channel provides for flow of the immiscible fluids, to accomplish pooling, coalescing, mixing, sorting, detection, etc., of the emulsion library.
摘要翻译： 本发明提供了可用于进行高通量筛选测定和组合化学的新型微流体装置和方法。 本发明提供了含有唯一标记的细胞，酶，核酸等的含水基乳液，其中乳液还包含引物，标记，探针和其它反应物。 油基载体流体在微流体装置上包封乳剂库，使得连续通道提供不混溶流体的流动，以实现乳液库的汇集，聚结，混合，分选，检测等。
-
公开(公告)号：US09441266B2
公开(公告)日：2016-09-13
申请号：US13773868
申请日：2013-02-22
CPC分类号： C12Q1/6816 , C12Q1/6827 , C12Q1/686 , C12Q1/6883 , C12Q1/6886 , C12Q2600/172 , C12Q2563/159 , C12Q2565/629 , C12Q2537/143 , C12Q2531/113 , C12Q2535/131
摘要： The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.
-
公开(公告)号：US09399797B2
公开(公告)日：2016-07-26
申请号：US13460762
申请日：2012-04-30
CPC分类号： C12Q1/686 , C12Q2565/629
摘要： The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, a method for determining the nucleic acid make-up of a sample is provided.
摘要翻译： 本发明一般涉及基于液滴的数字PCR和使用其进行目标核酸分析的方法。 在某些实施方案中，提供了用于测定样品的核酸组成的方法。
-
公开(公告)号：US09366632B2
公开(公告)日：2016-06-14
申请号：US13866126
申请日：2013-04-19
发明人： Darren R. Link , Qun Zhong , Andrew Watson
CPC分类号： G01N21/6486 , C12Q1/686 , C12Q2563/159 , C12Q2565/629
摘要： The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.
-
8.发明授权公开(公告)号：US09266104B2
公开(公告)日：2016-02-23
申请号：US13371217
申请日：2012-02-10
申请人： Darren Roy Link
发明人： Darren Roy Link
CPC分类号： B01L3/0265 , B01L3/502784 , B01L7/52 , B01L2200/0642 , B01L2200/0673 , B01L2300/0819 , B01L2300/1827 , B01L2300/185
摘要： The present invention provides thermocycling devices useful for amplification of nucleic acids in droplets. The thermocycling device utilizes the flow of one or more fluids through a main compartment at temperatures sufficient to conduct a polymerase chain reaction. Methods of amplifying nucleic acids in droplets are also provided.
摘要翻译： 本发明提供了可用于液滴中核酸扩增的热循环装置。 热循环装置利用在足以进行聚合酶链式反应的温度下通过主隔室的一种或多种流体的流动。 还提供了扩大液滴核酸的方法。
-
公开(公告)号：US09074242B2
公开(公告)日：2015-07-07
申请号：US13026120
申请日：2011-02-11
CPC分类号： C12Q1/6816 , C12Q1/6827 , C12Q1/686 , C12Q1/6883 , C12Q1/6886 , C12Q2600/172 , C12Q2563/159 , C12Q2565/629 , C12Q2537/143 , C12Q2531/113 , C12Q2535/131
摘要： The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.
-
公开(公告)号：US08658430B2
公开(公告)日：2014-02-25
申请号：US13554655
申请日：2012-07-20
申请人： Benjamin J. Miller , Qun Zhong , Darren Roy Link
发明人： Benjamin J. Miller , Qun Zhong , Darren Roy Link
IPC分类号： G01N1/10
CPC分类号： C12Q1/6806 , B01F3/0807 , B01F13/0062 , B01F15/00357 , B01L3/502761 , B01L7/525 , B01L2200/025 , B01L2200/0647 , B01L2200/141 , B01L2200/143 , B01L2200/148 , B01L2300/0867 , B01L2300/14 , B01L2400/0487 , B05B1/02 , B05B1/08 , B05B1/26 , B05B7/0012 , Y10T436/2575
摘要： The invention generally relates to methods and systems for manipulating droplet size. In certain aspects, the invention provides methods for manipulating droplet size that include forming droplets of aqueous fluid surrounded by an immiscible carrier fluid, and manipulating droplet size during the forming step by adjusting pressure exerted on the aqueous fluid or the carrier fluid.
摘要翻译： 本发明一般涉及用于操纵液滴尺寸的方法和系统。 在某些方面，本发明提供了用于操纵液滴尺寸的方法，其包括形成由不混溶的载体流体包围的水性流体的液滴，并且在成形步骤期间通过调节施加在含水流体或载体流体上的压力来操纵液滴尺寸。
-
-
-
-
-
-
-
-
-
搜索结果
58 条记录 (耗时 0.067 秒)
