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    SUBSTRATES WITH STABLE SURFACE CHEMISTRY FOR BIOLOGICAL MEMBRANE ARRAYS AND METHOD FOR FABRICATING THEREOF
    89.
    发明申请
    SUBSTRATES WITH STABLE SURFACE CHEMISTRY FOR BIOLOGICAL MEMBRANE ARRAYS AND METHOD FOR FABRICATING THEREOF 审中-公开
    用于生物膜阵列的稳定表面化学物质的基底及其制备方法

    公开(公告)号：US20090215650A1

    公开(公告)日：2009-08-27

    申请号：US12437893

    申请日：2009-05-08

    Applicant: Ye Fang ,  Anthony G. Frutos ,  Joydeep Lahiri

    Inventor： Ye Fang ,  Anthony G. Frutos ,  Joydeep Lahiri

    IPC: C40B40/10

    CPC classification number: G01N33/54393 ,  B01J19/0046 ,  B01J2219/00385 ,  B01J2219/00527 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00635 ,  B01J2219/00659 ,  B01J2219/0074 ,  C40B60/14 ,  G01N33/54366

    Abstract: The present invention provides a method for preparing a physically stable array of biological membranes, including membrane proteins, on a surface, and the resultant article of manufacture. The method comprises providing a substrate; creating either a polar surface or reactive surface by coating the substrate with a material that either: (1) enhances the stability of lipid spots during withdrawing through a water/air interface and washing and drying protocols; or (2) gives rise to minimal non-specific binding of a labeled target to a background surface, and high specific binding to a probe receptor in said membrane array, or (3) both; and depositing an array of biological-membrane microspots on the substrate. The method may further comprise applying a reagent that includes a soluable protein to stabilize the biological membranes on the surface. Also provided is an article having biological-membrane microspots that are associated in a stable fashion with a substrate surface embodying these properties.

    Abstract translation: 本发明提供了一种在表面上制备物理上稳定的生物膜阵列，包括膜蛋白的方法，以及所得制品。 该方法包括提供基底; 通过用以下材料涂覆基材来产生极性表面或反应性表面：（1）在通过水/空气界面排出并洗涤和干燥方案期间提高脂质斑点的稳定性; 或（2）导致标记的靶与背景表面的最小非特异性结合，以及所述膜阵列中与探针受体的高特异性结合，或（3）两者; 以及在衬底上沉积生物膜微点阵列。 该方法还可以包括施加包含可溶蛋白质的试剂以稳定表面上的生物膜。 还提供了具有生物膜微点的物品，其以稳定的方式与体现这些性质的基底表面相关联。

    Novel method for high-throughput integrated chemical and biochemical reactions
    90.
    发明申请
    Novel method for high-throughput integrated chemical and biochemical reactions 审中-公开
    高通量综合化学和生化反应的新方法

    公开(公告)号：US20090203009A1

    公开(公告)日：2009-08-13

    申请号：US12315385

    申请日：2008-12-03

    Applicant: Marinus Gerardus Johannus Van Beuningen ,  Pieter Jacob Boender

    Inventor： Marinus Gerardus Johannus Van Beuningen ,  Pieter Jacob Boender

    IPC: C12Q1/68 ,  G01N33/53

    CPC classification number: G01N33/551 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00317 ,  B01J2219/00351 ,  B01J2219/00353 ,  B01J2219/00416 ,  B01J2219/00421 ,  B01J2219/00533 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00628 ,  B01J2219/0063 ,  B01J2219/00635 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00659 ,  B01J2219/00707 ,  B01J2219/00722 ,  B01J2219/00725 ,  C12Q1/6837 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  Y10S436/805 ,  C12Q2565/629

    Abstract: The present invention relates to methods for monitoring in a high through-put fashion a multitude of molecular reaction processes, using a substrate, said substrate comprising a plurality of micro-channels, wherein each micro-channel has inlet and outlet open ends on opposing sides of said substrate, and wherein said micro-channels comprise at least one first reaction component, comprising the steps of: (a) contacting said micro-channels of said solid substrate with a sample, said contacting being via said inlet open ends, wherein said sample comprises an analyte, under conditions that allow said analyte to be specifically retained within said micro-channels by said first reaction component; (b) optionally removing excess of sample via said outlet open ends; (c) contacting the retained analyte with at least one second reaction component, said second reaction component being different from said first reaction component as defined in step (a), under conditions that allow a molecular reaction to take place, said reaction producing a signal; (d) detecting said signal and reading-out reaction results; (e) removing second reaction components via said outlet open ends; (f) repeating steps (c) to (e) at least once, wherein said at least one second reaction component as defined in (c) may be changed in composition; and, final detecting and reading-out of reaction results. The present invention also relates to the uses thereof as well as to microarrays and kits for performing said methods of the invention.

    Abstract translation: 本发明涉及以高通量方式监测多种分子反应过程的方法，使用基底，所述基底包括多个微通道，其中每个微通道在相对侧具有入口和出口开口端 ，并且其中所述微通道包括至少一个第一反应组分，包括以下步骤：（a）使所述固体基质的所述微通道与样品接触，所述接触通过所述入口开口端，其中所述 在允许所述分析物被所述第一反应组分特异性保留在所述微通道内的条件下，样品包含分析物; （b）任选地通过所述出口开口端去除过量的样品; （c）使保留的分析物与至少一个第二反应组分接触，所述第二反应组分与步骤（a）中所定义的所述第一反应组分不同，在允许发生分子反应的条件下，所述反应产生信号 ; （d）检测所述信号并读出反应结果; （e）经由所述出口开口端去除第二反应组分; （f）重复步骤（c）至（e）至少一次，其中如（c）中定义的所述至少一种第二反应组分可以在组成上改变; 并最终检测和阅读反应结果。 本发明还涉及其用途以及用于执行本发明的所述方法的微阵列和试剂盒。

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