Promoters and utilization thereof
    17.
    发明申请

    公开(公告)号:US20060277623A1

    公开(公告)日:2006-12-07

    申请号:US11500877

    申请日:2006-08-09

    CPC分类号: C12N15/67 C12N15/8216

    摘要: The present invention relates to improved promoters and utilization thereof, in particular to promoters which are improved so as not to undergo methylation in the course of constructing transformants, and utilization thereof. The improved promoters of the present invention are represented by the following (1) and (2): (1) a DNA comprising the nucleotide sequences shown in SEQ ID NOS: 1 to 4; (2) a DNA comprising a nucleotide sequence consisting of the nucleotide sequences shown in SEQ ID NOS 1 to 4, wherein one to several nucleotides are deleted, added or inserted and the deleted, added or inserted sequence is free from any consecutive sequences represented by CG, CAG, CTG, CCG or CGG; the DNA having promoter activity. According to the present invention, the expression efficiency of a structural gene can be enhanced even in a plant, e.g. chrysanthemum, which has weak expression of the structural gene by a cauliflower mosaic virus 35S promoter which has been considered a high expression promoter for plants.

    Promoters and utilization thereof
    18.
    发明授权
    Promoters and utilization thereof 失效
    促进剂及其利用

    公开(公告)号:US07115420B2

    公开(公告)日:2006-10-03

    申请号:US10220612

    申请日:2001-02-23

    IPC分类号: C12N15/82

    CPC分类号: C12N15/67 C12N15/8216

    摘要: The present invention relates to improved promoters and utilization thereof, in particular to promoters which are improved so as not to undergo methylation in the course of constructing transformants, and utilization thereof. According to the present invention, the expression efficiency of a structural gene can be enhanced even in a plant, e.g. chrysanthemum, which has weak expression of the structural gene by a cauliflower mosaic virus 35S promoter which as been considered a high expression promoter for plants.

    摘要翻译: 本发明涉及改进的启动子及其利用,特别是涉及在构建转化体的过程中不被甲基化改进的促进剂及其应用。 根据本发明,即使在植物中也能够提高结构基因的表达效率,例如, 菊花通过被认为是植物的高表达启动子的花椰菜花叶病毒35S启动子具有弱结构基因的表达。

    Method of growing epitaxial layers
    20.
    发明授权
    Method of growing epitaxial layers 失效
    生长外延层的方法

    公开(公告)号:US5476811A

    公开(公告)日:1995-12-19

    申请号:US409944

    申请日:1995-03-23

    摘要: A method of growing a plurality of epitaxial layers each having a property which is different from each other simultaneously on a common substrate comprises steps of forming at least a first crystal surface and a second crystal surface which are crystallographically non-equivalent to each other on the substrate, introducing particles comprising constituent elements of the epitaxial layers into a region in the vicinity of the substrate, the particles including at least metal-organic molecules containing one of the elements constituting the epitaxial layers, decomposing the metal-organic molecules such that the layer constituting element therein is released as a result of the decomposition, and depositing the aforesaid particles including the element released by the decomposition of the metal-organic molecules on the first and second crystal surfaces so that a first epitaxial layer and a second epitaxial layer, respectively differing in properties from each other, are grown on respective the first and second crystal surfaces, the step of deposition being performed such that the growth of the first and second epitaxial layers is controlled by the decomposition of the metal-organic molecule.

    摘要翻译: 在公共衬底上同时生长具有彼此不同的性质的多个外延层的方法包括以下步骤:至少形成在第一晶体表面和第二晶体表面上的晶体学上彼此不相同的 衬底,将包含外延层的构成元素的粒子引入到衬底附近的区域中,所述颗粒至少包含含有构成外延层的元素之一的金属有机分子,分解金属 - 有机分子,使得层 作为分解的结果释放其中的构成元素,并且将包含通过金属 - 有机分子分解释放的元素的上述颗粒沉积在第一和第二晶体表面上,使得分别具有第一外延层和第二外延层 不同的性质彼此分别生长 第一和第二晶体表面,进行沉积步骤使得通过金属 - 有机分子的分解来控制第一和第二外延层的生长。