公开(公告)号：US20180148766A1

公开(公告)日：2018-05-31

申请号：US15568471

申请日：2016-02-26

Applicant: AJ INNUSCREEN GMBH

Inventor： Timo Hillebrand ,  Thorsten Stroh

IPC: C12Q1/6806 ,  B01L3/02 ,  G01N1/40

CPC classification number: C12N15/1006 ,  B01L3/0275 ,  B01L2200/023 ,  B01L2200/0631 ,  B01L2200/0647 ,  B01L2300/0854 ,  B01L2300/16 ,  C12N15/1013 ,  C12Q1/6806 ,  G01N1/405 ,  G01N35/10

Abstract: Device and method for automated extraction of nucleic acids, comprising a body that can be immersed partly or completely in a reaction cavity, characterized in that at least the part immersed in the reaction cavity has a non-smooth surface. After lysis, an organic substance, preferably alcohols or ketones, is mixed with a biological sample. This mixture is now brought into contact with a material characterized by a non-smooth surface. Under these conditions, nucleic acids are adsorbed on the surface of the material being used. Thereupon washing steps with known alcoholic washing solutions may be carried out. After drying, the adsorbed nucleic acid is detached from the material by addition of water or a buffer of low salt concentration, whereupon it can be used for downstream applications.

公开(公告)号：US20180147573A1

公开(公告)日：2018-05-31

申请号：US15707908

申请日：2017-09-18

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Amy L. Hiddessen ,  Donald A. Masquelier ,  Kevin D. Ness ,  Benjamin J. Hindson ,  Anthony J. Makarewicz, Jr. ,  Erin R. Chia ,  Billy W. Colston, Jr. ,  Serge Saxonov ,  Svilen S. Tzonev ,  Michael Y. Lucero ,  Ryan T. Koehler

IPC: B01L3/00 ,  B01L9/00 ,  B01F13/00 ,  C12Q1/686 ,  B01F13/10 ,  B01F3/08 ,  B01F5/02 ,  B01F5/00 ,  G01N35/08

CPC classification number: B01L3/50273 ,  B01F3/0807 ,  B01F5/0085 ,  B01F5/0256 ,  B01F13/0062 ,  B01F13/0071 ,  B01F13/1022 ,  B01L3/502 ,  B01L3/502761 ,  B01L3/502784 ,  B01L3/52 ,  B01L9/527 ,  B01L2200/025 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2200/14 ,  B01L2200/16 ,  B01L2300/0609 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/0861 ,  B01L2400/049 ,  C12Q1/686 ,  G01N35/08

Abstract: Devices and methods for generating droplets. An exemplary device comprises a substantially planar base portion including a bottom surface having a plurality of microfluidic channels formed therein as recessed regions of the bottom surface. The device also comprises a plurality of protrusions projecting from a top surface of the base portion and each formed integrally with the base portion. The device further comprises a sample well, a carrier well, and a droplet well. Each well has an upper portion created by one of the protrusions. A cover layer is attached to the bottom surface of the base portion and seals a bottom side of each microfluidic channel.

公开(公告)号：US09977039B2

公开(公告)日：2018-05-22

申请号：US15644124

申请日：2017-07-07

Applicant: HemoSonics LLC ,  Karen Platt

Inventor： Francesco Viola ,  William F. Walker ,  Gregory V. Browne ,  Robert S. Magyar ,  Bjarne Hansen ,  Christopher G. Denny

IPC: G01N30/96 ,  G01N33/86 ,  B01L3/00 ,  G01N29/024 ,  G01N29/22 ,  G01N35/00

CPC classification number: G01N33/86 ,  B01L3/5027 ,  B01L3/502761 ,  B01L3/527 ,  B01L2200/04 ,  B01L2200/0647 ,  B01L2300/02 ,  B01L2300/024 ,  B01L2300/027 ,  B01L2300/0627 ,  B01L2300/0654 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/123 ,  B01L2300/14 ,  B01L2300/16 ,  B01L2300/1805 ,  B01L2400/0436 ,  B01L2400/0439 ,  B01L2400/0487 ,  B01L2400/049 ,  B01L2400/0666 ,  G01N29/024 ,  G01N29/222 ,  G01N35/00 ,  G01N2800/224

Abstract: Provided are devices, systems and methods for evaluation of hemostasis. Also provided are sound focusing assemblies.

公开(公告)号：US09968933B2

公开(公告)日：2018-05-15

申请号：US15673955

申请日：2017-08-10

Applicant: The University of Chicago

Inventor： Rustem F. Ismagilov ,  Joshua David Tice ,  Helen Song Baca ,  Lewis Spencer Roach

IPC: B01L3/00 ,  B01L7/00 ,  B01J19/00 ,  B01F3/08 ,  B01F5/06 ,  B01F13/00 ,  G01N15/10 ,  B01F5/04

CPC classification number: B01L3/502784 ,  B01F3/0861 ,  B01F5/0403 ,  B01F5/0646 ,  B01F5/0647 ,  B01F13/0071 ,  B01F2215/0037 ,  B01J19/0046 ,  B01J19/0093 ,  B01J2219/00286 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00599 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00736 ,  B01J2219/0074 ,  B01J2219/00756 ,  B01J2219/00783 ,  B01J2219/00837 ,  B01J2219/0086 ,  B01J2219/00867 ,  B01J2219/00869 ,  B01J2219/00889 ,  B01J2219/00891 ,  B01J2219/00975 ,  B01J2219/00977 ,  B01L3/502746 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2300/0867 ,  B01L2300/0883 ,  B01L2400/0424 ,  B01L2400/0448 ,  B01L2400/0454 ,  B01L2400/0487 ,  B82Y30/00 ,  C12P19/34 ,  C12Q1/44 ,  G01N15/1404 ,  G01N15/1484 ,  G01N35/08 ,  G01N35/085 ,  G01N2015/1409 ,  Y02A90/26 ,  Y10T436/118339 ,  Y10T436/12 ,  Y10T436/2575

Abstract: The present invention provides microfabricated substrates and methods of conducting reactions within these substrates. The reactions occur in plugs transported in the flow of a carrier-fluid.

公开(公告)号：US09964490B2

公开(公告)日：2018-05-08

申请号：US14851046

申请日：2015-09-11

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Matthew Beaudet ,  Jill Hendrickson ,  David Hagen ,  Rich Meyer

IPC: G01N21/76 ,  G01N21/64 ,  B01L7/00 ,  G01N15/14 ,  G01N35/00 ,  B01L3/00

CPC classification number: G01N21/6486 ,  B01L3/5082 ,  B01L7/52 ,  B01L2200/0647 ,  B01L2200/143 ,  G01N21/6428 ,  G01N21/6452 ,  G01N2015/1486 ,  G01N2021/6441 ,  G01N2035/00782 ,  Y10T436/143333

Abstract: The present invention relates to devices and methods for measuring the quantity of multiple analytes in a sample. The device is designed such that each of the analyte sensing elements is configured to measure the quantity of a predetermined analyte and where the machine executable instructions are configured to select the proper analyte sensing element corresponding to the analyte to be measured.

公开(公告)号：US09943846B2

公开(公告)日：2018-04-17

申请号：US15087721

申请日：2016-03-31

Applicant: The University of British Columbia

Inventor： Pieter R. Cullis ,  Igor V. Jigaltsev ,  James R. Taylor ,  Timothy Leaver ,  Andre Wild ,  Nathan Maurice Belliveau

IPC: A61F13/00 ,  B01L3/00 ,  A61K9/107 ,  A61K9/127 ,  A61K9/14 ,  A61K31/704 ,  A61K47/44 ,  A61K49/00 ,  B01F5/06 ,  B01F13/00 ,  B01F3/08 ,  A61J3/00

CPC classification number: B01L3/50273 ,  A61J3/00 ,  A61K9/1075 ,  A61K9/127 ,  A61K9/1271 ,  A61K9/1277 ,  A61K9/14 ,  A61K31/704 ,  A61K47/44 ,  A61K49/0002 ,  B01F3/0807 ,  B01F5/061 ,  B01F5/0647 ,  B01F13/0059 ,  B01F2005/0636 ,  B01L3/502715 ,  B01L2200/0605 ,  B01L2200/0647 ,  B01L2300/06 ,  B01L2300/0848 ,  B01L2300/0867 ,  B01L2300/18 ,  Y10T428/2982

Abstract: Limit size lipid nanoparticles, methods for using the lipid nanoparticles, and methods and systems for making limit size lipid nanoparticles.

公开(公告)号：US20180074060A1

公开(公告)日：2018-03-15

申请号：US15818333

申请日：2017-11-20

Applicant: CYTONOME/ST, LLC

Inventor： John R. Gilbert ,  Hugh Lewis ,  Derek Beaupre ,  Jaishree Trikha ,  Manish Deshpande

IPC: G01N33/577 ,  B01L3/00 ,  C12M1/00 ,  B01L9/00 ,  G01N15/10 ,  G01N15/14 ,  B01L5/00

CPC classification number: G01N33/577 ,  B01L3/502761 ,  B01L3/502776 ,  B01L5/00 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2200/082 ,  B01L2200/10 ,  B01L2300/0809 ,  B01L2300/0864 ,  B01L2400/0487 ,  C12M47/04 ,  G01N15/1056 ,  G01N15/1484 ,  G01N2015/1486 ,  G01N2015/149 ,  Y10T436/2575

Abstract: A single disposable cartridge for performing a process on a particle, such as particle sorting, encapsulates all fluid contact surfaces in the cartridge for use with microfluidic particle processing technology. The cartridge interfaces with an operating system for effecting particle processing. The encapsulation of the fluid contact surfaces insures, improves or promotes operator isolation and/or product isolation. The cartridge may employ any suitable technique for processing particles.

公开(公告)号：US20180074039A1

公开(公告)日：2018-03-15

申请号：US15560028

申请日：2016-03-23

Applicant: The Univeristy of North Carolina at Chapel Hill ,  Cornell University ,  Board of Supervisors of Louisiana State University and Agricultural and Mechanical College

Inventor： Steven A. Soper ,  Francis Barany ,  Sunggook Park ,  Michael Murphy ,  Collin J. Mckinney ,  John William Efcavitch ,  Mateusz Hupert

IPC: G01N33/487 ,  C12Q1/68 ,  B01L3/00

CPC classification number: G01N33/48721 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0645 ,  B01L2300/0861 ,  B01L2300/0896 ,  C12M1/40 ,  C12M23/16 ,  C12Q1/6806 ,  C12Q1/6837 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2561/125 ,  C12Q2565/629 ,  C12Q2565/631

Abstract: The present invention is directed to a device that comprises a biomolecular processor and one or more nanotubes. Each biomolecular processor comprises a bioreactor chamber defined by a solid substrate, a plurality of spaced support structures within said bioreactor chamber and attached to the solid substrate, and one or more capture molecules immobilized to some or all of said plurality of spaced support structures, said one or more capture molecules suitable to bind to a portion of a target nucleic acid molecule in a sample. The device also comprises one or more nanotubes defined by the solid substrate and fluidically coupled to the bioreactor chamber.

公开(公告)号：US09909102B2

公开(公告)日：2018-03-06

申请号：US15037191

申请日：2014-11-18

Applicant: PLATOD ,  ECOLE SUPÉRIEURE DE PHYSIQUE ET DE CHIMIE INDUSTRIELLES DE LA VILLE DE PARIS

Inventor： Dominique Baruch ,  Antoine Pierre Marin Blin ,  Aurelie Magniez ,  Sonia Chassac ,  Anne Le Goff ,  Mathilde Reyssat

IPC: C12M3/00 ,  C12N5/078 ,  B01L3/00 ,  G01N15/10 ,  A61K35/12 ,  G01N15/00

CPC classification number: C12N5/0644 ,  A61K2035/124 ,  B01L3/502715 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2200/0663 ,  B01L2300/0809 ,  B01L2300/0848 ,  B01L2300/0861 ,  B01L2300/16 ,  B01L2400/086 ,  C12N2501/125 ,  C12N2501/145 ,  C12N2521/00 ,  C12N2533/50 ,  G01N15/1056 ,  G01N2015/0084 ,  G01N2015/1081

Abstract: The invention relates to a fluidic device for producing platelets from a suspension of megakaryocytes or their fragments, comprising a production chamber comprising at least one channel in which a suspension of megakaryocytes is introduced to flow from its inlet to its outlet wherein said channel is textured with a plurality of obstacles on at least one portion of its inner surface. The invention is further directed to an ex vivo method for producing platelets from megakaryocytes using a fluidic device as defined above.

公开(公告)号：US09897532B2

公开(公告)日：2018-02-20

申请号：US14552256

申请日：2014-11-24

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Dino Di Carlo ,  Daniel R. Gossett ,  Henry T. K. Tse

IPC: G01N15/14 ,  G06F19/10 ,  G01N33/574 ,  C12Q1/02 ,  B01L3/00 ,  G01N33/487 ,  G01N15/00 ,  G01N15/10

CPC classification number: G01N15/1463 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502776 ,  B01L2200/0605 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2300/0654 ,  B01L2300/0864 ,  C12Q1/02 ,  G01N15/1404 ,  G01N15/1459 ,  G01N15/1484 ,  G01N33/487 ,  G01N33/574 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/1415 ,  G01N2015/149 ,  G01N2015/1495 ,  G01N2015/1497 ,  G01N2800/7028 ,  G06F19/10

Abstract: A system is disclosed that enables the automated measurement of cellular mechanical parameters at high throughputs. The microfluidic device uses intersecting flows to create an extensional flow region where the cells undergo controlled stretching. Cells are focused into streamlines prior to entering the extensional flow region. In the extensional region, each cell's deformation is measured with an imaging device. Automated image analysis extracts a range of independent biomechanical parameters from the images. These may include cell size, deformability, and circularity. The single cell data that is obtained may then be used to in a variety of ways. Scatter density plots of deformability and circularity may be developed and displayed for the user. Mechanical parameters such as deformability and circularity may be gated or thresholded to identify certain cells of interest or sub-populations of interest. Similarly, the mechanical data obtained using the device may be used as cell signatures.